论文信息 - A DNA-BINDING PEPTIDE FROM A PHAGE DISPLAY LIBRARY

A DNA-BINDING PEPTIDE FROM A PHAGE DISPLAY LIBRARY

A DNA-binding peptide was selected from a random peptide phage display library. For competitive elution using the DNA methyltransferase M · TaqI in the selection step, a biotin-labeled duplex oligodeoxyribonucleotide containing the 5′-TCGA-3′ recognition sequence of M · TaqI was employed. Nine of ten phages selected were found to have the same deduced amino acid sequence SVSVGMKPSPRP. The selected phage binds to DNA, as demonstrated in an ELISA.

J. Wölcke | E. Weinhold | E Weinhold | J Wölcke

[1] S. Klimašauskas,et al. 2-Aminopurine as a fluorescent probe for DNA base flipping by methyltransferases. , 1998, Nucleic acids research.

[2] K. Mosbach,et al. Selection of peptides with affinity for single stranded DNA using a phage display library. , 1994, Biochemical and Biophysical Research Communications - BBRC.

[3] E. Weinhold,et al. Higher binding affinity of duplex oligodeoxynucleotides containing 1,2-dideoxy-D-ribose to the N6-adenine DNA methyltransferase M center dot TaqI supports a base flipping mechanism , 1999 .

[4] J. Scott,et al. Searching for peptide ligands with an epitope library. , 1990, Science.

[5] J. Devlin,et al. Random peptide libraries: a source of specific protein binding molecules. , 1990, Science.