In Vitro picosecond ultrasonics in a single cell

Ultrasonics signals at frequencies 5.7±0.1 and 6.8±0.1GHz are measured in two organelles of a single vegetal cell in vitro with a picosecond ultrasonic technique. Using standard values for cell optical index, ultrasound velocities of 1.6±0.1 and 2.0±0.1μm∕ns are measured from several signals recorded in the vacuole and in the nucleus of a single Allium cepa cell, respectively. A 1μm lateral and 0.25μm depth resolution is attained.

[1]  P K Hansma,et al.  Measuring the viscoelastic properties of human platelets with the atomic force microscope. , 1996, Biophysical journal.

[2]  P. Bongrand,et al.  Localization of calcium and microfilament changes in mechanically stressed cells , 1990, Cell Biophysics.

[3]  L. W. Kessler,et al.  Acoustic Microscope operating at 100 MHz , 1971, Nature.

[4]  Jürgen Bereiter-Hahn,et al.  Mechanical Principles of Architecture of Eukaryotic Cells , 1987 .

[5]  J Beuthan,et al.  The spatial variation of the refractive index in biological cells. , 1996, Physics in medicine and biology.

[6]  G. Fischer.  Numerical data and functional relationships in science and technology , 1987 .

[7]  H. Rack,et al.  Titanium alloys in total joint replacement--a materials science perspective. , 1998, Biomaterials.

[8]  R. Gazzinelli,et al.  Measuring optical and mechanical properties of a living cell with defocusing microscopy. , 2006, Biophysical journal.

[9]  Erich Sackmann,et al.  Dynamic reflection interference contrast (RIC-) microscopy : a new method to study surface excitations of cells and to measure membrane bending elastic moduli , 1987 .

[10]  P. H. Yap,et al.  Refractive index measurement of single living cells using on-chip Fabry-Pérot cavity , 2006 .

[11]  F. Guillemot,et al.  Design of new titanium alloys for orthopaedic applications , 2006, Medical and Biological Engineering and Computing.

[12]  E. Evans,et al.  Cortical shell-liquid core model for passive flow of liquid-like spherical cells into micropipets. , 1989, Biophysical journal.

[13]  Humphrey J. Maris,et al.  PICOSECOND ULTRASONIC STUDY OF PHONON REFLECTION FROM SOLID-LIQUID INTERFACES , 1997 .

[14]  Bernard Perrin,et al.  Nondestructive evaluation of micrometric diamond films with an interferometric picosecond ultrasonics technique , 2004 .

[15]  P Bongrand,et al.  Measuring the Lifetime of Bonds Made between Surface-linked Molecules (*) , 1995, The Journal of Biological Chemistry.

[16]  Torsten Feigl,et al.  Formation and direct writing of color centers in LiF using a laser-induced extreme ultraviolet plasma in combination with a Schwarzschild objective , 2005 .

[17]  Zeev Valy Vardeny,et al.  Coherent Phonon Generation and Detection by Picosecond Light Pulses , 1984 .

[18]  M. D. Ward,et al.  Real‐Time Measurement of Anchorage‐Dependent Cell Adhesion Using a Quartz Crystal Microbalance , 1993, Biotechnology progress.

[19]  B. Hadimioglu,et al.  Advances in superfluid helium acoustic microscopy , 1984 .

[20]  C F Quate,et al.  Acoustic microscopy of living cells. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[21]  W. Liang,et al.  Hyperbranched polyethyleneimine architecture onto poly(allylamine) by simple synthetic approach and the chelating characters , 2001 .

[22]  N O Petersen,et al.  Dependence of locally measured cellular deformability on position on the cell, temperature, and cytochalasin B. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[23]  P A Valberg,et al.  Magnetic particle motions within living cells. Measurement of cytoplasmic viscosity and motile activity. , 1987, Biophysical journal.

[24]  T. Kundu,et al.  Acoustic microscope lens modeling and its application in determining biological cell properties from single- and multi-layered cell models. , 2006, The Journal of the Acoustical Society of America.

[25]  Marie-Christine Durrieu,et al.  Strategies and results of atomic force microscopy in the study of cellular adhesion. , 2006, Micron.

[26]  A. Ashkin,et al.  Internal cell manipulation using infrared laser traps. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[27]  V. A. D. Grosso,et al.  Speed of Sound in Pure Water , 1972 .

[28]  A. Mastro,et al.  Study of Cellular Adhesion with Scanning Acoustic Microscopy , 2007, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[29]  W. Ford,et al.  Picosecond ultrasonic measurement of the velocity of phonons in water , 2005 .

[30]  Thomsen,et al.  Surface generation and detection of phonons by picosecond light pulses. , 1986, Physical review. B, Condensed matter.