Contributions of host and symbiont pigments to the coloration of reef corals

For a variety of coral species, we have studied the molecular origin of their coloration to assess the contributions of host and symbiont pigments. For the corals Catalaphyllia jardinei and an orange‐emitting color morph of Lobophyllia hemprichii, the pigments belong to a particular class of green fluorescent protein‐like proteins that change their color from green to red upon irradiation with ∼400 nm light. The optical absorption and emission properties of these proteins were characterized in detail. Their spectra were found to be similar to those of phycoerythrin from cyanobacterial symbionts. To unambiguously determine the molecular origin of the coloration, we performed immunochemical studies using double diffusion in gel analysis on tissue extracts, including also a third coral species, Montastrea cavernosa, which allowed us to attribute the red fluorescent coloration to green‐to‐red photoconvertible fluorescent proteins. The red fluorescent proteins are localized mainly in the ectodermal tissue and contribute up to 7.0% of the total soluble cellular proteins in these species. Distinct spatial distributions of green and cyan fluorescent proteins were observed for the tissues of M. cavernosa. This observation may suggest that differently colored green fluorescent protein‐like proteins have different, specific functions. In addition to green fluorescent protein‐like proteins, the pigments of zooxanthellae have a strong effect on the visual appearance of the latter species.

[1]  J. Wiedenmann,et al.  Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[2]  R. Rowan.  MOLECULAR SYSTEMATICS OF SYMBIOTIC ALGAE , 1991 .

[3]  A. Salih,et al.  Simultaneous Time Resolution of the Emission Spectra of Fluorescent Proteins and Zooxanthellar Chlorophyll in Reef-building Corals¶,† , 2003, Photochemistry and photobiology.

[4]  J. Wiedenmann,et al.  Molecular diversity of symbiotic algae at the latitudinal margins of their distribution: dinoflagellates of the genus Symbiodinium in corals and sea anemones , 2002 .

[5]  A. Miyawaki,et al.  An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[6]  Charles H. Mazel,et al.  Discovery of Symbiotic Nitrogen-Fixing Cyanobacteria in Corals , 2004, Science.

[7]  P. Falkowski,et al.  � 2003, by the American Society of Limnology and Oceanography, Inc. Green-fluorescent proteins in Caribbean corals , 2022 .

[8]  Franz Oswald,et al.  Identification of GFP-like Proteins in Nonbioluminescent, Azooxanthellate Anthozoa Opens New Perspectives for Bioprospecting , 2004, Marine Biotechnology.

[9]  S. Lukyanov,et al.  Fluorescent proteins from nonbioluminescent Anthozoa species , 1999, Nature Biotechnology.

[10]  川口 四郎.  On the physiology of reef corals 4 : study on the pigments , 1943 .

[11]  K. Spindler,et al.  Cracks in the beta-can: fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria). , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[12]  E. Gantt,et al.  PROBING PHYCOBILISOME STRUCTURE BY IMMUNO‐ELECTRON MICROSCOPY 1 , 1977 .

[13]  K. Lukyanov,et al.  Diversity and evolution of the green fluorescent protein family , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[14]  C. Mullineaux,et al.  Photophysical properties of Prochlorococcus marinus SS120 divinyl chlorophylls and phycoerythrin in vitro and in vivo , 2003, FEBS letters.

[15]  M. Matz,et al.  Adaptive Evolution of Multicolored Fluorescent Proteins in Reef-Building Corals , 2006, Journal of Molecular Evolution.

[16]  S. Lukyanov,et al.  GFP-like proteins as ubiquitous metazoan superfamily: evolution of functional features and structural complexity. , 2004, Molecular biology and evolution.

[17]  G Ulrich Nienhaus,et al.  A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria) , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M. Matz.  Amplification of representative cDNA pools from microscopic amounts of animal tissue. , 2003, Methods in molecular biology.

[19]  O. Hoegh‐Guldberg,et al.  Closely related Symbiodinium spp. differ in relative dominance in coral reef host communities across environmental, latitudinal and biogeographic gradients , 2004 .

[20]  Y Chen,et al.  Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far‐red fluorescence , 2000, FEBS letters.

[21]  P. Targowski,et al.  Fluorescence Polarization Studies of B‐Phycoerythrin Oriented in Polymer Film¶ , 2004, Photochemistry and photobiology.

[22]  O. Hoegh‐Guldberg.  Climate change, coral bleaching and the future of the world's coral reefs , 1999 .

[23]  G. Ulrich Nienhaus,et al.  Photoconvertible Fluorescent Protein EosFP: Biophysical Properties and Cell Biology Applications , 2006, Photochemistry and photobiology.

[24]  M. Matz,et al.  Molecular basis and evolutionary origins of color diversity in great star coral Montastraea cavernosa (Scleractinia: Faviida). , 2003, Molecular biology and evolution.

[25]  Guy Cox,et al.  Fluorescent pigments in corals are photoprotective , 2000, Nature.

[26]  M. Matz,et al.  Evolution of Coral Pigments Recreated , 2004, Science.

[27]  N. Marshall,et al.  Are Corals Colorful? , 2006, Photochemistry and photobiology.

[28]  J. Wiedenmann,et al.  The morphs of Anemonia aff. sulcata (Cnidaria, Anthozoa) in particular consideration of the ectodermal pigments , 1999 .

[29]  Franz Oswald,et al.  Targeted Green-Red Photoconversion of EosFP, a Fluorescent Marker Protein , 2005, Journal of biological physics.

[30]  J. Wiedenmann,et al.  Molecular diversity of symbiotic algae of the genus Symbiodinium (Zooxanthellae) in cnidarians of the Mediterranean Sea , 2006, Journal of the Marine Biological Association of the United Kingdom.

[31]  B. Brown,et al.  Coral bleaching--capacity for acclimatization and adaptation. , 2003, Advances in marine biology.

[32]  J. Wiedenmann,et al.  EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[33]  J. Wiedenmann,et al.  Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family , 2006, Expert review of proteomics.

[34]  O. Hoegh-Guldberg,et al.  Major colour patterns of reef-building corals are due to a family of GFP-like proteins , 2001, Coral Reefs.

[35]  G. F. Humphrey,et al.  New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton , 1975 .

[36]  B. Willis,et al.  Flexibility in Algal Endosymbioses Shapes Growth in Reef Corals , 2004, Science.

[37]  G. Ulrich Nienhaus,et al.  Photoactivation in green to red converting EosFP and other fluorescent proteins from the GFP family , 2006, SPIE BiOS.