Application of Flow Cytometry for Rapid Determination of Cell Number of Viable Bacteria.

The application of the Flow cytometry (FCM) technique for rapid determination of cell number of viable bacteria suspended in seawateris described. A fluorescein diacetate was used to distinguish be tween viable cells and dead cells for various bacteria. The FCM technique was also used to discriminate betweenGram-positive and Gram-negative bacteriausing the LIVE BacLightTM Bacterial gram stain kit. A difference in the fluorescence scattergram between the two bacterial groups was observed. The FCM method provided rapid determination of cell number of viable bacteria in seawater. A good corre lationwas observed between the values determined by the FCM method and the colony counting method in the range of 105-109 cells/ml. One FCM assay could be completed within 60 s and the total assay time including the preparation of bacteria was within 35 min.

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