Rapid identification of prompt lactose-fermenting genera within the familyh Enterobacteriaceae.

Two hundred and two strains of prompt lactose-fermenting enterobacteria were examined, using 12 biochemical tests. In addition the susceptibility to several antibiotics was recorded. Identification on the basis of H2S production, ornithine decarboxylase activity, motility and citrate utilization (HOMoC) was compared with the results obtained using the classical IMV iC tests: indol, methyl red, Voges-Proskauer and citrate. Only the former set of reactions seemed suitable for clear-cut identification of the 4 genera Escherichia, Citrobacter, Enterobacter and Klebsiella. In showing low sensitivity to cephalotin Citrobacter and Enterobacter seemed to differ from the other 2 genera, and this could be used as an additional criterion for their identification.

[1]  W. Traub,et al.  Identification of Enterobacteriaceae in the Clinical Microbiology Laboratory , 1970, Applied microbiology.

[2]  J. Matsen,et al.  Characterization of ornithine decarboxylase-positive, nonmotile strains of the Klebsiella-Enterobacter group. , 1969, Applied microbiology.

[3]  J. Hall,et al.  Klebsiella, Enterobacter, and Serratia: biochemical differentiation and susceptibility to ampicillin and three cephalosporin derivatives. , 1969, Applied microbiology.

[4]  D. Amsterdam,et al.  New diagnostic system for the identification of lactose-fermenting gram-negative rods. , 1968, Applied microbiology.

[5]  M. Ramírez.  Differentiation of Klebsiella-Enterobacter (Aerobacter)-Serratia by biochemical tests and antibiotic susceptibility. , 1968, Applied microbiology.

[6]  Barry Al,et al.  Rapid identification of Escherichia, Klebsiella, and Enterobacter by use of a new urease test. , 1968 .

[7]  W. Ewing,et al.  Biochemical differentiation of the Enterobacteriaceae with the aid of lysine-iron-agar. , 1966, Applied microbiology.

[8]  P. R. Edwards,et al.  A Proposed Genus Enterobacter , 1960 .

[9]  W. Ewing,et al.  The Principal Divisions and Groups of Enterobacteriaceae and Their Differentiation , 1960 .

[10]  Enterobacteriaceae Subcommittee.  Report of the Enterobacteriaceae Subcommittee of the nomenclature committee of the International Association of Microbiological Societies , 1958 .

[11]  W. B. Christensen,et al.  Urea Decomposition as a Means of Differentiating Proteus and Paracolon Cultures from Each Other and from Salmonella and Shigella Types , 1946, Journal of bacteriology.