Improvement of translation efficiency in an Escherichia coli cell-free protein system using cysteine.

[1]  Alexander S. Spirin,et al.  Cell-Free Protein Synthesis , 2007 .

[2]  J. Swartz,et al.  Total amino acid stabilization during cell-free protein synthesis reactions. , 2006, Journal of biotechnology.

[3]  Hiroshi Murakami,et al.  A highly flexible tRNA acylation method for non-natural polypeptide synthesis , 2006, Nature Methods.

[4]  M. Jewett,et al.  Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm , 2004, Biotechnology and bioengineering.

[5]  James Swartz,et al.  Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome. , 2004, Metabolic engineering.

[6]  Dominic Esposito,et al.  A novel cell-free protein synthesis system. , 2004, Journal of biotechnology.

[7]  Takuya Ueda,et al.  Cell-free translation reconstituted with purified components , 2001, Nature Biotechnology.

[8]  R G Kim,et al.  Expression-independent consumption of substrates in cell-free expression system from Escherichia coli. , 2000, Journal of biotechnology.

[9]  Dong-Myung Kim,et al.  Prolonging Cell‐Free Protein Synthesis by Selective Reagent Additions , 2000, Biotechnology progress.

[10]  Dong-Myung Kim,et al.  Prolonging cell-free protein synthesis with a novel ATP regeneration system. , 1999, Biotechnology and bioengineering.

[11]  T. Yamane,et al.  Phosphatase-immunodepleted cell-free protein synthesis system. , 1998, Journal of biotechnology.

[12]  V. Erdmann,et al.  The potentials of the in vitro protein biosynthesis system. , 1995, Journal of biotechnology.

[13]  J. Jonák,et al.  Site-directed mutagenesis of elongation factor Tu. The functional and structural role of residue Cys81. , 1992, European journal of biochemistry.

[14]  Walter E. Hill,et al.  The Ribosome : structure, function, and evolution , 1990 .

[15]  A. Spirin,et al.  A continuous cell-free translation system capable of producing polypeptides in high yield. , 1988, Science.

[16]  J. Bodley,et al.  Selective chemical modification of Escherichia coli elongation factor G: butanedione modification of an arginine essential for nucleotide binding. , 1977, Biochemistry.

[17]  J. Bodley,et al.  Selective chemical modification of Escherichia coli elongation factor G. N-Ethylmaleimide modification of a cysteine essential for nucleotide binding. , 1976, The Journal of biological chemistry.

[18]  K. Mann,et al.  Chemical and physical studies on the structure of Escherichia coli elongation factor G. , 1975, The Journal of biological chemistry.

[19]  B. Paterson,et al.  Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[20]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.