Effect of L-arginine on Function of Mitochondria in Ischemia - Reperfusion Myocardial Cell in Rabbits

Objective: To investigate the effect of L-arginine (L-Arg) on the function of myocardial mitochondria during myocardia ischemia - reperfusion (IR). Methods: Dividing randomly the 30 rabbits into three groups (n=10): control group (C), myocardia ischemiareperfusion group (IR) and L-Arginine pretreatment group (L-Arg+IR). The relevant parameters, including myocardial mitochondria respiratory function, Ca2+ concentration ([Ca2+]), malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity, myocardial adenosine triphosphate (ATP), Adenosine diphosphate (ADP), adenosine monophosphate (AMP) content, the total amount of AMP (TAN, TAN=ATP+ADP+AMP) and energy charge (EC, EC=1/2ADP+ATP/TAN) were determined, respectively. Results: The mitochondria respiratory control rate (RCR), III state respiration rate (V3), and SOD in group L-Arg +IR were significantly higher than those in group IR, while IV state respiration rate (V4) , [Ca2+] m, and MDA were obviously lower than those of group IR, the levels of ATP, ADP, TNA and the EC of myocardium showed significantly higher than those in group IR. There were no significant differences in terms of V3, V4, SOD, MDA, AMP and TAN between group L-Arg+IR and group C. Conclusion: It indicated that L-arginine can reduce the level of the oxygen free radicals and partly attenuatecalcium overload to improve the function of myocardial mitochondria during myocardium ischemia reperfusion injury.

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