Rearrangements within human spliceosomes captured after exon ligation.
暂无分享,去创建一个
[1] P. Coltri,et al. Cwc24p Is a General Saccharomyces cerevisiae Splicing Factor Required for the Stable U2 snRNP Binding to Primary Transcripts , 2012, PloS one.
[2] J. Enderlein,et al. Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS. , 2012, RNA.
[3] Ulrich Stelzl,et al. Dynamic protein-protein interaction wiring of the human spliceosome. , 2012, Molecular cell.
[4] Debjani Saha,et al. Saccharomyces cerevisiae NineTeen Complex (NTC)-associated Factor Bud31/Ycr063w Assembles on Precatalytic Spliceosomes and Improves First and Second Step Pre-mRNA Splicing Efficiency* , 2012, The Journal of Biological Chemistry.
[5] C. Will,et al. Spliceosome structure and function. , 2011, Cold Spring Harbor perspectives in biology.
[6] Henning Urlaub,et al. Semiquantitative Proteomic Analysis of the Human Spliceosome via a Novel Two-Dimensional Gel Electrophoresis Method , 2011, Molecular and Cellular Biology.
[7] Robert J. Chalkley,et al. Breaking Up the C Complex Spliceosome Shows Stable Association of Proteins with the Lariat Intron Intermediate , 2011, PloS one.
[8] R. Reed,et al. The anti-tumor drug E7107 reveals an essential role for SF3b in remodeling U2 snRNP to expose the branch point-binding region. , 2011, Genes & development.
[9] J. Valcárcel,et al. Reduced fidelity of branch point recognition and alternative splicing induced by the anti-tumor drug spliceostatin A. , 2011, Genes & development.
[10] H. Stark,et al. 3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core. , 2010, Molecular cell.
[11] Henning Urlaub,et al. Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis. , 2010, RNA.
[12] P. Moynagh,et al. Cactin Targets the MHC Class III Protein IκB-like (IκBL) and Inhibits NF-κB and Interferon-regulatory Factor Signaling Pathways* , 2010, The Journal of Biological Chemistry.
[13] M. Jurica,et al. Spliceostatin A inhibits spliceosome assembly subsequent to prespliceosome formation , 2010, Nucleic acids research.
[14] Brendan MacLean,et al. Skyline: an open source document editor for creating and analyzing targeted proteomics experiments , 2010, Bioinform..
[15] John R Yates,et al. Release of SF3 from the intron branchpoint activates the first step of pre-mRNA splicing. , 2010, RNA.
[16] Robert J. Chalkley,et al. The role of exon sequences in C complex spliceosome structure. , 2009, Journal of molecular biology.
[17] Henning Urlaub,et al. The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome. , 2009, Molecular cell.
[18] H. Stark,et al. Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components , 2009, Nature Structural &Molecular Biology.
[19] H. Stark,et al. Exon, intron and splice site locations in the spliceosomal B complex , 2009, The EMBO journal.
[20] Peter R Baker,et al. In-depth Analysis of Tandem Mass Spectrometry Data from Disparate Instrument Types*S , 2008, Molecular & Cellular Proteomics.
[21] Henning Urlaub,et al. Conservation of the Protein Composition and Electron Microscopy Structure of Drosophila melanogaster and Human Spliceosomal Complexes , 2008, Molecular and Cellular Biology.
[22] B. Schwer. A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release. , 2008, Molecular cell.
[23] H. Urlaub,et al. Isolation of an active step I spliceosome and composition of its RNP core , 2008, Nature.
[24] M. Frilander,et al. RNA Ligation using T4 DNA Ligase , 2008 .
[25] M. Jurica,et al. A protein-based EM label for RNA identifies the location of exons in spliceosomes , 2008, Nature Structural &Molecular Biology.
[26] R. Sperling,et al. Exploring the architecture of the intact supraspliceosome using electron microscopy. , 2007, Journal of molecular biology.
[27] Melissa A. Mefford,et al. U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing. , 2007, Genes & development.
[28] M. Ares,et al. Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing. , 2007, Genes & development.
[29] Henning Urlaub,et al. Composition and three‐dimensional EM structure of double affinity‐purified, human prespliceosomal A complexes , 2007, The EMBO journal.
[30] Steven P Gygi,et al. Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry , 2007, Nature Methods.
[31] K. Gould,et al. Structural characterization of the fission yeast U5.U2/U6 spliceosome complex , 2007, Proceedings of the National Academy of Sciences.
[32] Henning Urlaub,et al. Protein composition of human mRNPs spliced in vitro and differential requirements for mRNP protein recruitment. , 2006, RNA.
[33] Eric W. Deutsch,et al. The PeptideAtlas project , 2005, Nucleic Acids Res..
[34] F. Yeh,et al. Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. , 2005, Genes & development.
[35] M. Moore,et al. Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core. , 2005, RNA.
[36] R. Lin,et al. Interaction between a G-Patch Protein and a Spliceosomal DEXD/H-Box ATPase That Is Critical for Splicing , 2004, Molecular and Cellular Biology.
[37] Nikolaus Grigorieff,et al. Three-dimensional structure of C complex spliceosomes by electron microscopy , 2004, Nature Structural &Molecular Biology.
[38] D. S. McPheeters,et al. Spatial Organization of Protein-RNA Interactions in the Branch Site-3′ Splice Site Region during pre-mRNA Splicing in Yeast , 2003, Molecular and Cellular Biology.
[39] Nikolaus Grigorieff,et al. Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. , 2002, RNA.
[40] K. Gould,et al. Proteomics Analysis Reveals Stable Multiprotein Complexes in Both Fission and Budding Yeasts Containing Myb-Related Cdc5p/Cef1p, Novel Pre-mRNA Splicing Factors, and snRNAs , 2002, Molecular and Cellular Biology.
[41] H. Le Hir,et al. The spliceosome deposits multiple proteins 20–24 nucleotides upstream of mRNA exon–exon junctions , 2000, The EMBO journal.
[42] J D Beggs,et al. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. , 2000, Genetics.
[43] R. Steward,et al. Cactin, a conserved protein that interacts with the Drosophila IκB protein Cactus and modulates its function , 2000, Mechanisms of Development.
[44] D. S. McPheeters,et al. Interaction of the yeast DExH-box RNA helicase prp22p with the 3' splice site during the second step of nuclear pre-mRNA splicing. , 2000, Nucleic acids research.
[45] W Chiu,et al. EMAN: semiautomated software for high-resolution single-particle reconstructions. , 1999, Journal of structural biology.
[46] R. Reed,et al. The RNA splicing factor hSlu7 is required for correct 3′ splice-site choice , 1999, Nature.
[47] B. Kastner,et al. Combined Biochemical and Electron Microscopic Analyses Reveal the Architecture of the Mammalian U2 snRNP , 1999, The Journal of cell biology.
[48] M. Moore,et al. The C-terminal region of hPrp8 interacts with the conserved GU dinucleotide at the 5' splice site. , 1999, RNA.
[49] J. Xie,et al. Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation , 1998, The EMBO journal.
[50] C. H. Gross,et al. Prp22, a DExH‐box RNA helicase, plays two distinct roles in yeast pre‐mRNA splicing , 1998, The EMBO journal.
[51] R. Lin,et al. Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing , 1996, Molecular and cellular biology.
[52] R. Reed,et al. Identification of proteins that interact with exon sequences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly , 1996, Molecular and cellular biology.
[53] M. Ares,et al. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits , 1996, Molecular and cellular biology.
[54] J. Reyes,et al. The canonical GU dinucleotide at the 5' splice site is recognized by p220 of the U5 snRNP within the spliceosome. , 1996, RNA.
[55] M. Ares,et al. CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145. , 1996, Genes & development.
[56] J. Beggs,et al. snRNA interactions at 5' and 3' splice sites monitored by photoactivated crosslinking in yeast spliceosomes. , 1995, RNA.
[57] C. Guthrie,et al. Prp16p, Slu7p, and Prp8p interact with the 3' splice site in two distinct stages during the second catalytic step of pre-mRNA splicing. , 1995, RNA.
[58] J. Beggs,et al. Extensive interactions of PRP8 protein with the 5′ and 3′ splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA. , 1995, The EMBO journal.
[59] J. Manley,et al. A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing. , 1995, Genes & development.
[60] J. Beggs,et al. Interaction of the yeast splicing factor PRP8 with substrate RNA during both steps of splicing. , 1995, Nucleic acids research.
[61] J. Steitz,et al. A base-pairing interaction between U2 and U6 small nuclear RNAs occurs in > 150S complexes in HeLa cell extracts: implications for the spliceosome assembly pathway. , 1993, Proceedings of the National Academy of Sciences of the United States of America.
[62] J. Steitz,et al. Site-specific cross-linking of mammalian U5 snRNP to the 5' splice site before the first step of pre-mRNA splicing. , 1992, Genes & development.
[63] C. Guthrie,et al. A conformational rearrangement in the spliceosome is dependent on PRP16 and ATP hydrolysis. , 1992, The EMBO journal.
[64] H. Sawa,et al. Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast. , 1992, Proceedings of the National Academy of Sciences of the United States of America.
[65] Hiten D. Madhani,et al. A novel base-pairing interaction between U2 and U6 snRNAs suggests a mechanism for the catalytic activation of the spliceosome , 1992, Cell.
[66] J. Steitz,et al. Interactions of small nuclear RNA's with precursor messenger RNA during in vitro splicing. , 1992, Science.
[67] M. Ares,et al. Efficient association of U2 snRNPs with pre-mRNA requires an essential U2 RNA structural element. , 1991, Genes & development.
[68] J. Manley,et al. Base pairing between U2 and U6 snRNAs is necessary for splicing of a mammalian pre-mRNA , 1991, Nature.
[69] A. Weiner,et al. Genetic evidence for base pairing between U2 and U6 snRNA in mammalian mRNA splicing , 1991, Nature.
[70] C. Guthrie,et al. PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome , 1991, Nature.
[71] J. Manley,et al. Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing. , 1989, Genes & development.
[72] Christine Guthrie,et al. Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA , 1987, Cell.
[73] A Leith,et al. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. , 1996, Journal of structural biology.
[74] M van Heel,et al. A new generation of the IMAGIC image processing system. , 1996, Journal of structural biology.