Highly sensitive protein detection based on aptamer probe and isothermal nicking enzyme assisted fluorescence signal amplification.

An isothermal and sensitive fluorescence assay for protein detection using aptamer-protein-aptamer conjugates based on nicking enzyme amplification has been developed, which was combined with magnetic microparticles separation, making this assay suitable for protein detection in biological samples.

[1]  M F Kubik,et al.  Oligonucleotide inhibitors of human thrombin that bind distinct epitopes. , 1997, Journal of molecular biology.

[2]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[3]  H. Kong,et al.  Engineering a nicking endonuclease N.AlwI by domain swapping , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[4]  Itamar Willner,et al.  Spotlighting of cocaine by an autonomous aptamer-based machine. , 2007, Journal of the American Chemical Society.

[5]  G. Wilson,et al.  Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI. , 2005, Journal of molecular biology.

[6]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[7]  Ciara K O'Sullivan,et al.  Displacement enzyme linked aptamer assay. , 2005, Analytical chemistry.

[8]  Yi Xiao,et al.  Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. , 2004, Journal of the American Chemical Society.

[9]  E. Wang,et al.  Simple and sensitive aptamer-based colorimetric sensing of protein using unmodified gold nanoparticle probes. , 2007, Chemical communications.

[10]  Y. Shao,et al.  A chronocoulometric aptamer sensor for adenosine monophosphate. , 2007, Chemical communications.

[11]  Itamar Willner,et al.  Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA. , 2006, Angewandte Chemie.

[12]  Guo-Li Shen,et al.  Reusable electrochemical sensing platform for highly sensitive detection of small molecules based on structure-switching signaling aptamers. , 2007, Analytical chemistry.

[13]  E. Wang,et al.  4-(Dimethylamino)butyric acid labeling for electrochemiluminescence detection of biological substances by increasing sensitivity with gold nanoparticle amplification. , 2005, Analytical chemistry.

[14]  Jun-Jie Zhu,et al.  DNA aptamer-based QDs electrochemiluminescence biosensor for the detection of thrombin. , 2009, Biosensors & bioelectronics.

[15]  Milan N Stojanovic,et al.  Aptamer-based colorimetric probe for cocaine. , 2002, Journal of the American Chemical Society.

[16]  X Chris Le,et al.  Aptamer-linked assay for thrombin using gold nanoparticle amplification and inductively coupled plasma-mass spectrometry detection. , 2009, Analytical chemistry.

[17]  C R Cantor,et al.  Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. , 1992, Science.

[18]  E. Vermaas,et al.  Selection of single-stranded DNA molecules that bind and inhibit human thrombin , 1992, Nature.

[19]  Jeong-O Lee,et al.  Aptamers as molecular recognition elements for electrical nanobiosensors , 2007, Analytical and bioanalytical chemistry.

[20]  Kazufumi Hosoda,et al.  A novel sequence-specific RNA quantification method using nicking endonuclease, dual-labeled fluorescent DNA probe, and conformation-interchangeable oligo-DNA. , 2008, RNA.

[21]  I. Willner,et al.  Supramolecular aptamer-thrombin linear and branched nanostructures. , 2008, Chemical communications.

[22]  A. Ellington,et al.  Aptamer beacons for the direct detection of proteins. , 2001, Analytical biochemistry.

[23]  Robert M. Corn,et al.  Fabrication and characterization of RNA aptamer microarrays for the study of protein–aptamer interactions with SPR imaging , 2006, Nucleic acids research.

[24]  H. Schluesener,et al.  Ultrasensitive detection of protein using an aptamer-based exonuclease protection assay. , 2004, Analytical chemistry.

[25]  M. Mascini,et al.  Aptamer-based detection of plasma proteins by an electrochemical assay coupled to magnetic beads. , 2007, Analytical chemistry.

[26]  P. He,et al.  A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles. , 2007, Biosensors & bioelectronics.

[27]  Da Xing,et al.  Magnetic bead and nanoparticle based electrochemiluminescence amplification assay for direct and sensitive measuring of telomerase activity. , 2009, Analytical chemistry.

[28]  Eun Jeong Cho,et al.  Real-time rolling circle amplification for protein detection. , 2007, Analytical chemistry.

[29]  Guo-Li Shen,et al.  Universal aptameric system for highly sensitive detection of protein based on structure-switching-triggered rolling circle amplification. , 2010, Analytical chemistry.

[30]  Henk Fidder,et al.  Intramolecular dimers: a new strategy to fluorescence quenching in dual-labeled oligonucleotide probes. , 2002, Journal of the American Chemical Society.