Principles and Applications of Fluorescence Spectroscopy

1. Absorption spectroscopy theory. 2. Determination of Calcofluor White Molar extinction coefficient value in absence and presence of alpha1-acid glycoprotein. 3. Determination of kinetic parameters of Lactate dehydrogenase (LDH). 4. Hydrolysis of p-nitropenyl-beta-D-galactoside with beta-galactosidase from E.coli. 5. Starch hydrolysis by amylase. 6. Detrermination of the pK of a dye. 7. Fluorescence spectroscopy principles. 8. Effect of the structure and the environment of a fluorophore on its absorption and fluorescence spectra. 9. Fluorophores characterization and importance in biology. 10. Fluorescence quenching. 11. Fluorescence polarization. 12. Interaction between ethidium bromide and DNA. 13. Lens culinaris agglutinin: dynamics and binding studies. 14. Forster energy transfer. 15. Binding of TNS on bovine serum albumin at pH 3 and pH 7. 16. Comet Test for environmental genotoxicity evaluation: a fluorescence microscopy application. 17. Questions and exercises

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