论文信息 - Changes in Amino Acid Content of Excised Leaves During Incubation. III. Role of Sugar in the Accumulation of Proline in Wilted Leaves.

Changes in Amino Acid Content of Excised Leaves During Incubation. III. Role of Sugar in the Accumulation of Proline in Wilted Leaves.

A previously reported accumulation of proline in wilted turnip leaves has been observed in 6 additional species representing 5 different families. The results of experiments on the conditions affecting the behavior of proline in wilted leaves were interpreted to mean that the supply and metabolism of available carbohydrate was essential for proline accumulation.The role of sugar in proline accumulation was determined by incubating wilted leaves with different levels of carbohydrate (produced either by variations in conditions of preillumination or by infiltration with sucrose). The results showed that proline accumulation was greater and most prolonged in wilted leaves with higher sugar and starch contents. No proline accumulation was observed in unwilted leaves. Also some inhibitors of glycolysis and of the tricarboxylic acid cycle prevented proline accumulation in wilted leaves.Based on these results, the interpretation of the role of sugar in proline accumulation is that the oxidation of sugars furnishes alpha-ketoglutarate and NAD(P)H for proline synthesis in wilted leaves.

C. J. Morris | John F. Thompson | Cecil R. Stewart

[1] J. F. Thompson,et al. Changes in amino Acid content of excised leaves during incubation I. The effect of water content of leaves and atmospheric oxygen level. , 1966, Plant physiology.

[2] A. W. Naylor,et al. Amino Acid and protein metabolism in bermuda grass during water stress. , 1966, Plant physiology.

[3] D. Routley. Proline Accumulation in Wilted Ladino Clover Leaves 1 , 1966 .

[4] Sofia Simmonds,et al. Biochemistry of the Amino Acids , 1966, Nature.

[5] R. Hochster,et al. Free proline in normal and in crown-gall tumor tissue of tomato and tobacco☆ , 1964 .

[6] P. Henckel. Physiology of Plants Under Drought , 1964 .

[7] B. Kessler,et al. Studies on Water Regime and Nitrogen Metabolism of Citrus Seedlings Grown under Water Stress. , 1964, Plant physiology.

[8] J. Greenstein,et al. Chemistry of the Amino Acids , 1961 .

[9] R. Gering,et al. The effect of mineral supply on the amino acid composition of plants. , 1960 .

[10] W S Iljin,et al. Drought Resistance in Plants and Physiological Processes , 1957 .

[11] A. Kemble,et al. Liberation of amino acids in perennial rye grass during wilting. , 1954, The Biochemical journal.

[12] F. Chinard. Photometric estimation of proline and ornithine. , 1952, The Journal of biological chemistry.

[13] H. Spoehr,et al. LEAF STARCH: ITS ISOLATION AND SOME OF ITS PROPERTIES , 1935 .

[14] C. B. Thorne. Metabolism of nitrogenous compounds. , 1956, Annual review of microbiology.