Techniques Used for Morphological and Ultrastructural Description from Teeth White-Tufted-Ear-Marmosets (Callithrix jacchus)

The scanning electron microscope (SEM) is a device capable of producing images of high magnification allowing a more detailed morphological analysis. Mineralized structures (hard), like teeth, can benefit from a differentiated protocol when submitted to the SEM technique, thus aiming at a considerable decrease of time, however when processed in routine histology techniques suffer from an increase in processing time due to decalcification. The family Callitrichidae is divides into six genera: Callithrix, Callimico, Cebuella, Saguinus, Leontopithecus and Mico. They have 32 teeth in the formula dental 2x: incisors 2/2; Canines 1/1, pre-molars 3/3 molar and 2/2. We used teeth of tufted marmosets white (Callithrix jacchus) for studies macroscopic, microscopic and ultrastructural. Considering the above and taking into account the importance of processing time and acceleration data capture, was adapted a protocol of the SEM to reduce processing time, and for histology was used descaling technique using formic acid, hydrochloric acid and distilled water. The results show the exact structures of the teeth and ensuring the method and analysis results.

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