Engineering strain-sensitive yellow fluorescent protein.

Various biological events including muscle contraction and vesicle transport can be described as a mechanical process. Many of the corresponding proteins are thus required to generate or sense a force. Here we describe a strain-sensitive yellow fluorescent protein (YFP) recombinant that can detect the intramolecular strains these proteins experience.

[1]  S. Iwai,et al.  Visualizing myosin–actin interaction with a genetically-encoded fluorescent strain sensor , 2008, Proceedings of the National Academy of Sciences.

[2]  Kevin Truong,et al.  Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics , 2006, Biotechnology Letters.

[3]  S J Remington,et al.  Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. , 1998, Structure.

[4]  Shimon Weiss,et al.  Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy , 2000, Nature Structural Biology.

[5]  H. Goodson,et al.  Motors and membrane traffic. , 1997, Current opinion in cell biology.

[6]  R. Vale,et al.  Kinesin Walks Hand-Over-Hand , 2004, Science.

[7]  Sol M Gruner,et al.  Alteration of citrine structure by hydrostatic pressure explains the accompanying spectral shift , 2008, Proceedings of the National Academy of Sciences.

[8]  B. Zemelman,et al.  PRIM: proximity imaging of green fluorescent protein-tagged polypeptides. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[9]  V. Muñoz,et al.  Folding dynamics and mechanism of β-hairpin formation , 1997, Nature.

[10]  Jane Clarke,et al.  Mechanical unfolding of proteins: insights into biology, structure and folding. , 2007, Current opinion in structural biology.

[11]  Michael P. Sheetz,et al.  Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility , 1985, Cell.

[12]  R. Tsien,et al.  green fluorescent protein , 2020, Catalysis from A to Z.

[13]  P. Alexander,et al.  Gene for an immunoglobulin-binding protein from a group G streptococcus , 1986, Journal of bacteriology.

[14]  Roger Y. Tsien,et al.  Crystal Structure of the Aequorea victoria Green Fluorescent Protein , 1996, Science.

[15]  W. Greenleaf,et al.  High-resolution, single-molecule measurements of biomolecular motion. , 2007, Annual review of biophysics and biomolecular structure.

[16]  R. Tsien,et al.  Reducing the Environmental Sensitivity of Yellow Fluorescent Protein , 2001, The Journal of Biological Chemistry.

[17]  Brian P Helmke,et al.  Mechanisms of mechanotransduction. , 2006, Developmental cell.

[18]  Chae Un Kim,et al.  Coupling of pressure-induced structural shifts to spectral changes in a yellow fluorescent protein. , 2009, Biophysical journal.

[19]  T. Yanagida,et al.  Motility of single one-headed kinesin molecules along microtubules. , 2001, Biophysical journal.

[20]  O. Shimomura,et al.  Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. , 1962, Journal of cellular and comparative physiology.

[21]  S. Iwai,et al.  Myosin‐actin interaction in Dictyostelium cells revealed by GFP‐based strain sensor and validated linear spectral unmixing , 2010, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[22]  D. Toomre,et al.  A new wave of cellular imaging. , 2010, Annual review of cell and developmental biology.

[23]  Taekjip Ha,et al.  Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics , 2010, Nature.

[24]  K. Schulten,et al.  Fluorescence-Force Spectroscopy Maps Two-Dimensional Reaction Landscape of the Holliday Junction , 2007, Science.

[25]  R Y Tsien,et al.  Understanding, improving and using green fluorescent proteins. , 1995, Trends in biochemical sciences.

[26]  William W. Ward,et al.  SPECTRAL PERTURBATIONS OF THE AEQUOREA GREEN‐FLUORESCENT PROTEIN , 1982 .