Isolation and characterization of psychrophiles producing cold‐active ‐galactosidase

Aims: The present study was conducted to screen for psychrophilic micro‐organisms that are able to hydrolyse lactose at low temperature, and to examine the cold‐active β‐galactosidase produced by the isolated psychrophilic micro‐organisms.

[1]  F. Schinner,et al.  Properties of cold-adapted microorganisms and their potential role in biotechnology , 1994 .

[2]  N. Willassen,et al.  Cold adapted enzymes. , 2000, Biotechnology annual review.

[3]  J. Loveland,et al.  Characterization of psychrotrophic microorganisms producing beta-galactosidase activities , 1994, Applied and environmental microbiology.

[4]  R. Y. Morita,et al.  PSYCHROPHILIC BACTERIA , 1959, Bacteriological reviews.

[5]  G. Feller,et al.  Enzymes from psychrophilic organisms , 1996 .

[6]  P. Sheridan,et al.  Biochemical and phylogenetic analyses of psychrophilic isolates belonging to the Arthrobacter subgroup and description of Arthrobacter psychrolactophilus, sp. nov. , 1999, Archives of Microbiology.

[7]  N. Russell,et al.  Molecular adaptations in psychrophilic bacteria: potential for biotechnological applications. , 1998, Advances in biochemical engineering/biotechnology.

[8]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[9]  J. Brenchley,et al.  Characterization of a psychrotrophic Arthrobacter gene and its cold-active beta-galactosidase , 1994, Applied and environmental microbiology.

[10]  T. Nakagawa,et al.  Cold-active pectinolytic activity of psychrophilic-basidiomycetous yeast Cystofilobasidium capitatum strain PPY-1. , 2002, Journal of bioscience and bioengineering.

[11]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[12]  M. Bentahir,et al.  Cold-adapted enzymes: from fundamentals to biotechnology. , 2000, Trends in biotechnology.

[13]  Y. Sakai,et al.  Regulation and Physiological Role of theDAS1 Gene, Encoding Dihydroxyacetone Synthase, in the Methylotrophic Yeast Candida boidinii , 1998, Journal of bacteriology.

[14]  E. Stackebrandt,et al.  Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample. , 2002, International journal of systematic and evolutionary microbiology.

[15]  K. Komagata,et al.  Cystite formation of Arthrobacter ureafaciens NRIC 0157 , 2000 .