Comparison Effects of Platelet-Rich Fibrin on Macrofat and Shuffling Fat Grafting.

Fat acquisition, processing, and grafting techniques are considered to be the main factors affecting the volume retention rate of fat grafting. Shuffling fat (SF) is obtained by refining macrofat (MF), both of which have been applied in autologous fat grafting. However, few studies have focused on comparison effects of platelet-rich fibrin (PRF) on MF and SF grafting. Grafts were prepared by mixing MF and SF with PRF or normal saline at a ratio of 5:1, and grafted into symmetric recipient sites of a mouse's buttock. Grafts were harvested and analyzed at 4, 8, and 12 weeks. Groups containing PRF showed high volume retention rates and microcosmic evaluation scores at each time point compared with the control groups. Although without improved volume retention rate, group of SF containing PRF showed superior microcosmic evaluations compared with group of MF containing PRF. The results demonstrated that both the efficacy of MF and SF grafting can be enhanced by PRF, with even better quality of grafts for the SF grafting.

[1]  M. Quirynen,et al.  The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane , 2018, Platelets.

[2]  Zuoliang Qi,et al.  Clinical Application of Platelet-Rich Fibrin in Plastic and Reconstructive Surgery: A Systematic Review , 2018, Aesthetic Plastic Surgery.

[3]  F. Lu,et al.  Improved Long-Term Volume Retention of Stromal Vascular Fraction Gel Grafting with Enhanced Angiogenesis and Adipogenesis , 2018, Plastic and reconstructive surgery.

[4]  Yunjun Liao,et al.  Adipose Extracellular Matrix/Stromal Vascular Fraction Gel: A Novel Adipose Tissue–Derived Injectable for Stem Cell Therapy , 2017, Plastic and reconstructive surgery.

[5]  M. Bircoll.  Improvement of Fat Graft Survival with Autologous Bone Marrow Aspirate and Bone Marrow Concentrate: A One-Step Method. , 2017, Plastic and reconstructive surgery.

[6]  U. Kandalam,et al.  Optimized Platelet‐Rich Fibrin With the Low‐Speed Concept: Growth Factor Release, Biocompatibility, and Cellular Response , 2017, Journal of periodontology.

[7]  Q. Wang,et al.  Improvement of Fat Graft Survival with Autologous Bone Marrow Aspirate and Bone Marrow Concentrate: A One-Step Method , 2016, Plastic and reconstructive surgery.

[8]  R. Miron,et al.  Comparative release of growth factors from PRP, PRF, and advanced-PRF , 2016, Clinical Oral Investigations.

[9]  I. Martin,et al.  Effects of Intersyringe Processing on Adipose Tissue and Its Cellular Components: Implications in Autologous Fat Grafting , 2015, Plastic and reconstructive surgery.

[10]  J. Rubin,et al.  Application of platelet-rich plasma and platelet-rich fibrin in fat grafting: basic science and literature review. , 2014, Tissue engineering. Part B, Reviews.

[11]  M. Friji.  Nanofat grafting: basic research and clinical applications. , 2014, Plastic and reconstructive surgery.

[12]  H. Declercq,et al.  Nanofat Grafting: Basic Research and Clinical Applications , 2013, Plastic and Reconstructive Surgery.

[13]  Jeffrey H. Lee,et al.  The Effect of Pressure and Shear on Autologous Fat Grafting , 2013, Plastic and reconstructive surgery.

[14]  S. O. Keyhan,et al.  Use of platelet-rich fibrin and platelet-rich plasma in combination with fat graft: which is more effective during facial lipostructure? , 2013, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[15]  B. Liu,et al.  The adjuvant use of stromal vascular fraction and platelet-rich fibrin for autologous adipose tissue transplantation. , 2013, Tissue engineering. Part C, Methods.

[16]  Jing Li,et al.  [The comparison of platelet-rich fibrin and platelet-rich plasma in releasing of growth factors and their effects on the proliferation and differentiation of adipose tissue-derived stem cells in vitro]. , 2012, Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology.

[17]  Y. Ullmann,et al.  Treating Fat Grafts with Human Endothelial Progenitor Cells Promotes Their Vascularization and Improves Their Survival in Diabetes Mellitus , 2012, Plastic and reconstructive surgery.

[18]  P. Tonnard,et al.  Sharp-needle intradermal fat grafting (SNIF). , 2012, Aesthetic surgery journal.

[19]  C. Malheiros,et al.  Increased survival of free fat grafts with platelet-rich plasma in rabbits. , 2010, Journal of plastic, reconstructive & aesthetic surgery : JPRAS.

[20]  D. M. Dohan Ehrenfest,et al.  Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies , 2009, Growth factors.

[21]  W. Xia,et al.  VEGF gene therapy for the survival of transplanted fat tissue in nude mice. , 2007, Journal of plastic, reconstructive & aesthetic surgery : JPRAS.

[22]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[23]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[24]  A. Dohan,et al.  Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. , 2006, Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics.

[25]  J. May,et al.  Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery. , 1989, Plastic and reconstructive surgery.