Staphylococcus epidermidis adhesion on surface-treated open-cell Ti6Al4V foams

Abstract The effect of alkali and nitric acid surface treatments on the adhesion of Staphylococcus epidermidis to the surface of 60% porous open-cell Ti6Al4V foam was investigated. The resultant surface roughness of foam particles was determined from the ground flat surfaces of thin foam specimens. Alkali treatment formed a porous, rough Na2Ti5O11 surface layer on Ti6Al4V particles, while nitric acid treatment increased the number of undulations on foam flat and particle surfaces, leading to the development of finer surface topographical features. Both surface treatments increased the nanometric-scale surface roughness of particles and the number of bacteria adhering to the surface, while the adhesion was found to be significantly higher in alkali-treated foam sample. The significant increase in the number of bacterial attachment on the alkali-treated sample was attributed to the formation of a highly porous and nanorough Na2Ti5O11 surface layer.

[1]  J. Evans,et al.  Optimising the Bioactivity of Alkaline-treated Titanium Alloy , 2002 .

[2]  R M Pilliar,et al.  The effect of sol-gel-formed calcium phosphate coatings on bone ingrowth and osteoconductivity of porous-surfaced Ti alloy implants. , 2004, Biomaterials.

[3]  M. Hamilton,et al.  Effects of Substratum Topography on Bacterial Adhesion. , 1998, Journal of colloid and interface science.

[4]  K. Dai,et al.  Berberine inhibits Staphylococcus Epidermidis adhesion and biofilm formation on the surface of titanium alloy , 2009, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[5]  Y. An,et al.  Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. , 1998, Journal of biomedical materials research.

[6]  Thomas J Webster,et al.  The relationship between the nanostructure of titanium surfaces and bacterial attachment. , 2010, Biomaterials.

[7]  M. Dunbar,et al.  Fibroblastic interactions with high-porosity Ti-6Al-4V metal foam. , 2007, Journal of Biomedical Materials Research. Part B - Applied biomaterials.

[8]  Y. Do Kim,et al.  Surface modification by alkali and heat treatments in titanium alloys. , 2002, Journal of biomedical materials research.

[9]  J. Planell,et al.  Growth of bioactive surfaces on titanium and its alloys for orthopaedic and dental implants , 2002 .

[10]  C. Ránninger,et al.  Influence of gas nitriding of Ti6Al4V alloy at high temperature on the adhesion of Staphylococcus aureus , 2006 .

[11]  R. Pilliar,et al.  Effect of surface chemistry on the rate of osseointegration of sintered porous-surfaced Ti-6Al-4V implants. , 2004, The International journal of oral & maxillofacial implants.

[12]  P. Cintas,et al.  Controlled silanization-amination reactions on the Ti6Al4V surface for biomedical applications. , 2013, Colloids and surfaces. B, Biointerfaces.

[13]  M. Neo,et al.  A porous bioactive titanium implant for spinal interbody fusion: an experimental study using a canine model. , 2007, Journal of neurosurgery. Spine.

[14]  Tadashi Kokubo,et al.  Mechanical properties and osteoconductivity of porous bioactive titanium. , 2005, Biomaterials.

[15]  Yu Zhang,et al.  Promoting Bone Mesenchymal Stem Cells and Inhibiting Bacterial Adhesion of Acid-Etched Nanostructured Titanium by Ultraviolet Functionalization , 2015 .

[16]  Tomiharu Matsushita,et al.  Osteoinductive porous titanium implants: effect of sodium removal by dilute HCl treatment. , 2006, Biomaterials.

[17]  L Ploux,et al.  The interaction of cells and bacteria with surfaces structured at the nanometre scale. , 2010, Acta biomaterialia.

[18]  Y. Leng,et al.  Spectroscopic analysis of titanium surface functional groups under various surface modification and their behaviors in vitro and in vivo. , 2008, Journal of biomedical materials research. Part A.

[19]  Y. Leng,et al.  A comparative study of electrochemical deposition and biomimetic deposition of calcium phosphate on porous titanium. , 2005, Biomaterials.

[20]  M. Textor,et al.  Surface characterization , 1999, Journal of materials science. Materials in medicine.

[21]  W. Zimmerli,et al.  Prosthetic-joint-associated infections , 2006 .

[22]  A. Chernikova,et al.  Correlation between bioactivity and structural properties of titanium dioxide coatings grown by atomic layer deposition , 2012 .

[23]  Yang Leng,et al.  Biomimetic calcium phosphate coatings on nitric-acid-treated titanium surfaces , 2007 .

[24]  T. Webster,et al.  Enhanced fibronectin adsorption on carbon nanotube/poly(carbonate) urethane: independent role of surface nano-roughness and associated surface energy. , 2007, Biomaterials.

[25]  Xiaojing Wang,et al.  Staphylococcus aureus adhesion to different implant surface coatings: An in vitro study , 2009 .

[26]  W. Zimmerli,et al.  Prosthetic-joint infections. , 2004, The New England journal of medicine.

[27]  H. Moriya,et al.  Enhanced fixation of implants by bone ingrowth to titanium fiber mesh: effect of incorporation of hydroxyapatite powder. , 2005, Journal of biomedical materials research. Part B, Applied biomaterials.

[28]  H. M. Kim,et al.  Preparation of bioactive Ti and its alloys via simple chemical surface treatment. , 1996, Journal of biomedical materials research.

[29]  Elena P Ivanova,et al.  The influence of nano-scale surface roughness on bacterial adhesion to ultrafine-grained titanium. , 2010, Biomaterials.

[30]  W. Teughels,et al.  Effect of material characteristics and/or surface topography on biofilm development. , 2006, Clinical oral implants research.

[31]  Stanley A. Brown,et al.  Implant site infection rates with porous and dense materials. , 1979, Journal of biomedical materials research.

[32]  T. Sculco.  The economic impact of infected joint arthroplasty. , 1995, Orthopedics.

[33]  B. Kasemo,et al.  Site-specific adhesion of Staphylococcus epidermidis (RP12) in Ti-Al-V metal systems. , 1994, Biomaterials.

[34]  Mamoru Mabuchi,et al.  Processing of biocompatible porous Ti and Mg , 2001 .

[35]  A. Taşdemirci,et al.  Processing and compression testing of Ti6Al4V foams for biomedical applications , 2009 .

[36]  Naoyuki Nomura,et al.  Mechanical properties of porous titanium compacts prepared by powder sintering , 2003 .

[37]  T. Kokubo,et al.  Development of bioactive materials based on surface chemistry , 2009 .

[38]  R M Pilliar,et al.  Porous-surfaced metallic implants for orthopedic applications. , 1987, Journal of biomedical materials research.

[39]  C. Wen,et al.  The importance of particle size in porous titanium and nonporous counterparts for surface energy and its impact on apatite formation. , 2009, Acta biomaterialia.

[40]  K. Neoh,et al.  Surface functionalization of titanium with hyaluronic acid/chitosan polyelectrolyte multilayers and RGD for promoting osteoblast functions and inhibiting bacterial adhesion. , 2008, Biomaterials.

[41]  R. Darouiche,et al.  Treatment of infections associated with surgical implants. , 2004, The New England journal of medicine.

[42]  M. Güden,et al.  The effect of surface treatment on CaP deposition of Ti6Al4V open cell foams in SBF solution , 2010 .

[43]  Elena P Ivanova,et al.  Do bacteria differentiate between degrees of nanoscale surface roughness? , 2011, Biotechnology journal.

[44]  Peter X Ma,et al.  Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment. , 2003, Journal of biomedical materials research. Part A.

[45]  S. Fujibayashi,et al.  Enhanced Osteoinductivity of Porous Titanium Implant by Sodium Removal , 2006 .

[46]  J. Jansen,et al.  Bone formation in calcium-phosphate-coated titanium mesh. , 2000, Biomaterials.

[47]  K. Kawanabe,et al.  Apatite layer-coated titanium for use as bone bonding implants. , 1997, Biomaterials.

[48]  Abhay Pandit,et al.  Fabrication methods of porous metals for use in orthopaedic applications. , 2006, Biomaterials.

[49]  H. Rack,et al.  Titanium alloys in total joint replacement--a materials science perspective. , 1998, Biomaterials.