Hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase onto a poly(aniline-co-N-methylthionine) film

Abstract In this present work, we developed a novel hydrogen peroxide (H 2 O 2 ) biosensor fabricated using electrochemical doping to immobilize hydrogen peroxidase (HRP) in a new conducting polymer, poly(aniline- co - N -methylthionine) (PAN-PNMThH). Amperometric detection of H 2 O 2 was evaluated by holding the PAN-PNMThH HRP electrode at −0.25 V (versus saturated calomel electrode (SCE)). PAN-PNMThH showed excellent redox activity and high porosity and acted as an electron transfer mediator. The biosensor had a wide linear response range from 5.0 μM to 60.0 mM H 2 O 2 with a sensitivity of 35 mA M −1  cm −2 , a detection limit of 3.2 μM (signal-to-noise ratio of 3) and an apparent Michaelis constant ( K M ) of 2.79 mM. The biosensor possessed good analytical performance and storage stability.

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