DNA-templated Au nanoclusters coupled with proximity-dependent hybridization and guanine-rich DNA induced quenching: a sensitive fluorescent biosensing platform for DNA detection

In this paper, the fluorescence signal of poly(A) DNA-templated Au nanoclusters (AuNCs) is found to be greatly quenched by photoinduced electron transfer (PET) when they are close to guanine (G)-rich DNA. Based on the findings, we have designed a low-cost fluorescence biosensing strategy for the sensitive detection of DNA. Highly luminescent and photo-stable poly(A) DNA-AuNCs were utilized as the fluorescent indicator and G-rich DNA was utilized as the fluorescent quencher. In the absence of target DNA, DNA-AuNCs failed to hybridize with the G-rich DNA and did not form the duplex DNA structure. Strong fluorescence intensity at 475 nm was observed due to the DNA-AuNCs being far away from the G-rich DNA. However, in the presence of target DNA, the DNA-AuNCs together with G-rich DNA could hybridize with the target DNA, leading to the 5′ terminus of the DNA-AuNCs and the 3′ terminus of G-rich DNA being in close proximity and promoting the cooperative hybridization. Therefore, a “Y” junction structure was formed and the G-rich sequences were brought close to the AuNCs. Therefore, the fluorescence intensity of the sensing system decreased significantly. Taking advantage of the poly(A) DNA-templated Au nanoclusters and G-rich DNA proximity-induced quenching, the strategy could be extended to determine other biomolecules by designing appropriate sequences of DNA probes.

[1]  Jian-hui Jiang,et al.  Light-up RNA aptamer enabled label-free protein detection via a proximity induced transcription assay. , 2018, Chemical communications.

[2]  J. Xie,et al.  Toward Total Synthesis of Thiolate-Protected Metal Nanoclusters. , 2018, Accounts of chemical research.

[3]  Yanming Liu,et al.  Development of Rapid and Label-Free Fluorescence Sensing of Tetracyclines in Milk Based on Poly(Adenine) DNA-Templated Au Nanoclusters , 2018, Food Analytical Methods.

[4]  Yanming Liu,et al.  DNA-templated Au nanoclusters and MnO2 sheets: a label-free and universal fluorescence biosensing platform , 2018 .

[5]  Zhenfeng Chen,et al.  A gold nanoparticle-based four-color proximity immunoassay for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification. , 2018, Chemical communications.

[6]  Yan Jin,et al.  Histone-DNA interaction: an effective approach to improve the fluorescence intensity and stability of DNA-templated Cu nanoclusters. , 2017, Chemical communications.

[7]  Wei Liu,et al.  A sensitive and real-time assay of restriction endonuclease activity and inhibition based on photo-induced electron transfer , 2017 .

[8]  Minghui Yang,et al.  Fluorescence assay of Fe (III) in human serum samples based on pH dependent silver nanoclusters , 2017 .

[9]  Yongming Guo,et al.  Fluorescent copper nanoparticles: recent advances in synthesis and applications for sensing metal ions. , 2016, Nanoscale.

[10]  Hai-Bo Wang,et al.  A fluorescent biosensor for protein detection based on poly(thymine)-templated copper nanoparticles and terminal protection of small molecule-linked DNA. , 2015, Biosensors & bioelectronics.

[11]  Xiaogang Qu,et al.  Metal nanoclusters: novel probes for diagnostic and therapeutic applications. , 2015, Chemical Society reviews.

[12]  Yunchao Wu,et al.  UV-Light-Induced Improvement of Fluorescence Quantum Yield of DNA-Templated Gold Nanoclusters: Application to Ratiometric Fluorescent Sensing of Nucleic Acids. , 2015, ACS applied materials & interfaces.

[13]  Qiang Zhao,et al.  Nanoparticles-free fluorescence anisotropy amplification assay for detection of RNA nucleotide-cleaving DNAzyme activity. , 2015, Analytical chemistry.

[14]  X. Chu,et al.  A new label-free and turn-on strategy for endonuclease detection using a DNA-silver nanocluster probe. , 2015, Talanta.

[15]  Zhihong Liu,et al.  Hairpin DNA probes based on target-induced in situ generation of luminescent silver nanoclusters. , 2014, Chemical communications.

[16]  Kemin Wang,et al.  Poly(thymine)-templated selective formation of fluorescent copper nanoparticles. , 2013, Angewandte Chemie.

[17]  Dong-shan Xiang,et al.  Multiplexed DNA detection with a composite molecular beacon based on guanine-quenching. , 2013, The Analyst.

[18]  Jian-hui Jiang,et al.  Double strand DNA-templated copper nanoparticle as a novel fluorescence indicator for label-free detection of polynucleotide kinase activity. , 2013, Biosensors & bioelectronics.

[19]  E. Wang,et al.  Photoinduced electron transfer of DNA/Ag nanoclusters modulated by G-quadruplex/hemin complex for the construction of versatile biosensors. , 2013, Journal of the American Chemical Society.

[20]  S. Yao,et al.  A simple adenosine fluorescent aptasensor based on the quenching ability of guanine , 2012 .

[21]  Hailun He,et al.  Fluorescence detection of adenosine triphosphate using smart probe. , 2012, Analytical biochemistry.

[22]  G. Shen,et al.  A proximity-dependent surface hybridization strategy for constructing an efficient signal-on electrochemical DNAzyme sensing system. , 2012, Chemical communications.

[23]  Xiaocui Zhu,et al.  In situ, real-time monitoring of the 3' to 5' exonucleases secreted by living cells. , 2012, Analytical chemistry.

[24]  Samir Kumar Pal,et al.  Copper Quantum Clusters in Protein Matrix: Potential Sensor of Pb 2+ Ion , 2022 .

[25]  Chengzhou Zhu,et al.  A simple and sensitive fluorescent sensing platform for Hg²+ ions assay based on G-quenching. , 2011, Talanta.

[26]  Lei Wang,et al.  A novel single-labeled fluorescent oligonucleotide probe for silver(I) ion detection based on the inherent quenching ability of deoxyguanosines. , 2011, The Analyst.

[27]  Andriy Mokhir,et al.  Selective dsDNA-templated formation of copper nanoparticles in solution. , 2010, Angewandte Chemie.

[28]  Jason J. Han,et al.  A DNA--silver nanocluster probe that fluoresces upon hybridization. , 2010, Nano letters.

[29]  Jian-hui Jiang,et al.  Electrochemical Aptasensor Based on Proximity-Dependent Surface Hybridization Assay for Protein Detection , 2009 .

[30]  Jian-hui Jiang,et al.  Electrochemical DNA biosensor based on the proximity-dependent surface hybridization assay. , 2009, Analytical chemistry.

[31]  Xinsheng Zhao,et al.  Aptamer biosensor for protein detection based on guanine-quenching , 2008 .

[32]  Guo-Li Shen,et al.  Electrochemical aptasensor based on proximity-dependent surface hybridization assay for single-step, reusable, sensitive protein detection. , 2007, Journal of the American Chemical Society.

[33]  Yoshio Saito,et al.  Design of a novel G-quenched molecular beacon: a simple and efficient strategy for DNA sequence analysis. , 2007, Chemical communications.

[34]  Michael Zuker,et al.  DINAMelt web server for nucleic acid melting prediction , 2005, Nucleic Acids Res..

[35]  Robert M Dickson,et al.  DNA-templated Ag nanocluster formation. , 2004, Journal of the American Chemical Society.

[36]  Y. Kamagata,et al.  Fluorescence-Quenching Phenomenon by Photoinduced Electron Transfer between a Fluorescent Dye and a Nucleotide Base , 2001, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.