Shaperone-Dependent Optimization of Expression in E. coli and Purification of Soluble Recombinant Lipid A Phosphatase LpxE from Francisella tularensis

[1]  I. Kurnaz.  Protein Production and Purification , 2015 .

[2]  A. Duschl,et al.  Residual Endotoxin Contaminations in Recombinant Proteins Are Sufficient to Activate Human CD1c+ Dendritic Cells , 2014, PloS one.

[3]  J. Beckwith,et al.  Folding LacZ in the Periplasm of Escherichia coli , 2014, Journal of bacteriology.

[4]  Germán L. Rosano,et al.  Recombinant protein expression in Escherichia coli: advances and challenges , 2014, Front. Microbiol..

[5]  M. Pichichero.  Protein carriers of conjugate vaccines , 2013, Human vaccines & immunotherapeutics.

[6]  Rino Rappuoli,et al.  Vaccines, reverse vaccinology, and bacterial pathogenesis. , 2013, Cold Spring Harbor perspectives in medicine.

[7]  P. Oyston,et al.  Protecting against plague: towards a next‐generation vaccine , 2013, Clinical and experimental immunology.

[8]  R. Curtis,et al.  Production of recombinant peptides as fusions with SUMO. , 2011, Protein expression and purification.

[9]  M. Wolfert,et al.  Differential Induction of Innate Immune Responses by Synthetic Lipid A Derivatives* , 2010, The Journal of Biological Chemistry.

[10]  Hayyoung Lee,et al.  The structural basis of lipopolysaccharide recognition by the TLR4–MD-2 complex , 2009, Nature.

[11]  C. Raetz,et al.  Purification and Characterization of the Lipid A 1-Phosphatase LpxE of Rhizobium leguminosarum* , 2009, Journal of Biological Chemistry.

[12]  C. Raetz,et al.  Periplasmic orientation of nascent lipid A in the inner membrane of an Escherichia coli LptA mutant , 2008, Proceedings of the National Academy of Sciences.

[13]  W. Yeh,et al.  LPS/TLR4 signal transduction pathway. , 2008, Cytokine.

[14]  K. Gunsalus,et al.  Protein production and purification , 2008, Nature Methods.

[15]  Thomas C. Mitchell,et al.  The Vaccine Adjuvant Monophosphoryl Lipid A as a TRIF-Biased Agonist of TLR4 , 2007, Science.

[16]  M. Kundi.  New hepatitis B vaccine formulated with an improved adjuvant system , 2007, Expert review of vaccines.

[17]  A. Skerra,et al.  A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. , 2006, Protein engineering, design & selection : PEDS.

[18]  S. Howie,et al.  "Dirty little secrets"--endotoxin contamination of recombinant proteins. , 2006, Immunology letters.

[19]  H. P. Sørensen,et al.  Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli , 2005 .

[20]  Xiaoyuan Wang,et al.  Periplasmic Cleavage and Modification of the 1-Phosphate Group of Helicobacter pylori Lipid A* , 2004, Journal of Biological Chemistry.

[21]  Xiaoyuan Wang,et al.  MsbA Transporter-dependent Lipid A 1-Dephosphorylation on the Periplasmic Surface of the Inner Membrane , 2004, Journal of Biological Chemistry.

[22]  Suzanne R. Kalb,et al.  Expression Cloning and Biochemical Characterization of a Rhizobium leguminosarum Lipid A 1-Phosphatase* , 2003, Journal of Biological Chemistry.

[23]  W. McKinstry,et al.  Removal of endotoxin by reverse phase HPLC abolishes anti-endothelial cell activity of bacterially expressed plasminogen kringle 5. , 2003, BioTechniques.

[24]  C. Chou,et al.  Roles of DegP in Prevention of Protein Misfolding in the Periplasm upon Overexpression of Penicillin Acylase in Escherichia coli , 2003, Journal of bacteriology.

[25]  R. Coler,et al.  Taking toll: lipid A mimetics as adjuvants and immunomodulators. , 2002, Trends in microbiology.

[26]  M. Qiao,et al.  Efficient Insertion Mutagenesis Strategy for Bacterial Genomes Involving Electroporation of In Vitro-Assembled DNA Transposition Complexes of Bacteriophage Mu , 2002, Applied and Environmental Microbiology.

[27]  P. Moingeon,et al.  Towards the rational design of Th1 adjuvants. , 2001, Vaccine.

[28]  M. Wilson,et al.  Removal of tightly bound endotoxin from biological products. , 2001, Journal of biotechnology.

[29]  F. Baneyx.  Recombinant protein expression in Escherichia coli. , 1999, Current opinion in biotechnology.

[30]  R A Mathies,et al.  DNA sequencing using capillary array electrophoresis. , 1992, Analytical chemistry.

[31]  A. Hasegawa,et al.  Structural features that influence the ability of lipid A and its analogs to abolish expression of suppressor T cell activity , 1992, Infection and immunity.

[32]  F. Studier,et al.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. , 1986, Journal of molecular biology.

[33]  T. Itoh,et al.  The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmids , 1983, Cell.

[34]  N. Qureshi,et al.  Purification and structural determination of nontoxic lipid A obtained from the lipopolysaccharide of Salmonella typhimurium. , 1982, The Journal of biological chemistry.

[35]  Adalberto Pessoa,et al.  Methods of endotoxin removal from biological preparations: a review. , 2007, Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques.

[36]  H. Kolb,et al.  Lipopolysaccharide Heat Shock Protein 60 : Specific Binding of Volker , 2005 .

[37]  J. T. Ulrich,et al.  Monophosphoryl lipid A as an adjuvant. Past experiences and new directions. , 1995, Pharmaceutical biotechnology.

[38]  J. T. Ulrich,et al.  Monophosphoryl Lipid A as an Adjuvant , 1995 .