Investigation of quercetin binding sites on chloroplast coupling factor 1.

The quercetin binding sites on spinach chloroplast coupling factor 1 (CF1) have been investigated using direct and competitive binding, stopped-flow, temperature-jump, and fluorescence resonance energy transfer measurements. It was found that 8-anilino-1-naphthalensulfonic acid (ANS) competes with quercetin binding at two sites on the solubilized enzyme which are distinct from the two tight nucleotide binding sites and the 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) reactive site. The bimolecular association of quercetin with CF1 is too fast to measure directly and is followed by two slower conformational changes. The distances from the tight nucleotide sites to the quercetin-ANS sites were estimated as 40-48 A by fluorescence resonance energy transfer using 1,N6-ethenoadenosine diphosphate and 1,N6-ethenoadenylyl imidodiphosphate as donors and quercetin as the acceptor. The distance from the quercetin-ANS site to the NBD-C1 reactive site was found to be about 30 A using ANS as a donor and NBD-C1 reacted with a tyrosine group on CF1 as the energy acceptor. A model is proposed for the relative location of these sites on CF1.

[1]  G G Hammes,et al.  Characterization of nucleotide binding sites on chloroplast coupling factor 1. , 1975, Biochemistry.

[2]  L. Cantley,et al.  Fluorescence energy transfer between ligand binding sites on chloroplast coupling factor 1. , 1975, Biochemistry.

[3]  N. Nelson,et al.  Partial resolution of the enzymes catalyzing photophosphorylation. XV. Approaches to the active site of coupling factor I. , 1975, The Journal of biological chemistry.

[4]  G. Hammes,et al.  Fluorescence energy transfer between ligand binding sites on aspartate transcarbamylase. , 1975, Biochemistry.

[5]  Robert E. Dale,et al.  Intramolecular distances determined by energy transfer. Dependence on orientational freedom of donor and acceptor , 1974 .

[6]  E. Racker,et al.  Effects of quercetin and F1 inhibitor on mitochondrial ATPase and energy-linked reactions in submitochondrial particles. , 1974, Biochimica et biophysica acta.

[7]  L. Cantley,et al.  Activation of beef heart mitochondrial adenosine triphosphatase by 2,4-dinitrophenol. , 1973, Biochemistry.

[8]  D A Hilborn,et al.  An on-line computer system for the acquisition and analysis of temperature jump data. , 1973, Computers and biomedical research, an international journal.

[9]  E. Racker,et al.  Partial resolution of the enzymes catalyzing photophosphorylation. IX. Studies on the subunit structure of coupling factor 1 from chloroplasts. , 1972, The Journal of biological chemistry.

[10]  Efraim Racker,et al.  [49] Preparation and assay of chloroplast coupling factor CF1 , 1971 .

[11]  F. Farron.  Isolation and properties of a chloroplast coupling factor and heat-activated adenosine triphosphatase. , 1970, Biochemistry.

[12]  T. G. Scott,et al.  Synthetic spectroscopic models related to coenzymes and base pairs. V. Emission properties of NADH. Studies of fluorescence lifetimes and quantum efficiencies of NADH, AcPyADH, [reduced acetylpyridineadenine dinucleotide] and simplified synthetic models , 1970 .

[13]  A. Bruni,et al.  Effect of quercetin on membrane-linked activities. , 1969, Biochemical pharmacology.

[14]  G. Hammes,et al.  Binding of phosphate ligands to ribonuclease A. , 1968, Biochemistry.

[15]  E. Moudrianakis,et al.  Function of the "quantasome" in photosynthesis: structure and properties of membrane-bound particle active in the dark reactions of photophosphorylation. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[16]  L. Stryer,et al.  The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites. , 1965, Journal of molecular biology.

[17]  H. Lardy,et al.  ANTIBIOTIC STUDIES. II. INHIBITION OF PHOSPHORYL TRANSFER IN MITOCHONDRIA BY OLIGOMYCIN AND AUROVERTIN. , 1964, Biochemistry.

[18]  S. McGlynn,et al.  Polarization of the Luminescence of Phenanthrene , 1962 .

[19]  C. A. Parker,et al.  Correction of fluorescence spectra and measurement of fluorescence quantum efficiency , 1960 .

[20]  T. Főrster,et al.  10th Spiers Memorial Lecture. Transfer mechanisms of electronic excitation , 1959 .

[21]  G. Rodney,et al.  The effect of a series of fiavonoids on hyaluronidase and some other related enzymes. , 1950 .