Tentative identification of RNA‐dependent RNA polymerases of dsRNA viruses and their relationship to positive strand RNA viral polymerases

Amino acid sequence stretches similar to the four most conserved segments of positive strand RNA viral RNA‐dependent RNA polymerases have been identified in proteins of four dsRNA viruses belonging to three families, i.e. P2 protein of bacteriophage φ6 (Cystoviridae), RNA 2 product of infectious bursa disease virus (Birnaviridae), λ3 protein of reovirus, and VP1 of bluetongue virus (Reoviridae). High statistical significance of the observed similarity was demonstrated, allowing identification of these proteins as likely candidates for RNA‐dependent RNA polymerases. Based on these observations, and on the previously reported sequence similarity between the RNA polymerases of a yeast dsRNA virus and those of positive strand RNA viruses, a possible evolutionary relationship between the two virus classes is discussed.

[1]  P. Gottlieb,et al.  Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase , 1988, Journal of virology.

[2]  A. Azad,et al.  Genomic structure of tbe large RNA segment of infectious bursal disease virus , 1986 .

[3]  J. Wiener,et al.  The sequences of the reovirus serotype 1, 2, and 3 L1 genome segments and analysis of the mode of divergence of the reovirus serotypes. , 1989, Virology.

[4]  L. Mindich,et al.  Nucleotide sequence of the small double-stranded RNA segment of bacteriophage phi 6: novel mechanism of natural translational control , 1986, Journal of virology.

[5]  N. Tordo,et al.  Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses. , 1988, Virology.

[6]  H. Müller,et al.  The two segments of the infectious bursal disease virus genome are circularized by a 90,000-Da protein. , 1987, Virology.

[7]  L. Mindich,et al.  RNA synthesis during infection with bacteriophage phi6. , 1976, Virology.

[8]  Mathew E. Diamond,et al.  Identification of a putative RNA dependent RNA polymerase encoded by a yeast double stranded RNA virus , 1988, Nucleic Acids Res..

[9]  D. Drayna,et al.  Activation and characterization of the reovirus transcriptase: genetic analysis , 1982, Journal of virology.

[10]  E. Koonin,et al.  Birnavirus RNA polymerase is related to polymerases of positive strand RNA viruses. , 1988, Nucleic acids research.

[11]  P. Gottlieb,et al.  Nucleotide sequence of the middle dsRNA segment of bacteriophage phi 6: placement of the genes of membrane-associated proteins. , 1988, Virology.

[12]  F. Brown,et al.  Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. , 1984, Virology.

[13]  V. Pozdnyakov,et al.  Accelerated method for comparing amino acid sequences with allowance for possible gaps. Plotting optimum correspondence paths. , 2009, International journal of peptide and protein research.

[14]  J. Köck,et al.  Structure and sequence of the gene for the largest subunit of trypanosomal RNA polymerase III. , 1988, Nucleic acids research.

[15]  A. Ishihama,et al.  Viral RNA polymerases. , 1988, CRC critical reviews in biochemistry.

[16]  I. Macreadie,et al.  Sequence of the small double-stranded RNA genomic segment of infectious bursal disease virus and its deduced 90-kDa product. , 1988, Virology.

[17]  S. Cook,et al.  Measles virus L protein evidences elements of ancestral RNA polymerase. , 1988, Virology.

[18]  B. Moss,et al.  Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[19]  T. Shioda,et al.  Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. , 1986, Nucleic acids research.

[20]  P. Roy,et al.  Evidence for genetic relationship between RNA and DNA viruses from the sequence homology of a putative polymerase gene of bluetongue virus with that of vaccinia virus: conservation of RNA polymerase genes from diverse species. , 1988, Nucleic acids research.