Selection Strategies for the Development of Maize Introgression Populations

Introgression libraries are valuable resources for QTL detection and breeding, but their development is costly and time-consuming. Selection strategies for the development of introgression populations with a limited number of individuals and high-throughput (HT) marker assays are required. The objectives of our simulation study were to design and compare selection strategies for the development of maize introgression populations of 100 lines with population sizes of 360–720 individuals per generation for different DH and crossing schemes. Pre-selection for complete donor chromosomes or donor chromosome halves reduced the number of simultaneous backcross programs. The investigated crossing and selection schemes differed considerably with respect to their suitability to create introgression populations with clearly separated, evenly distributed target donor chromosome segments. DH crossing schemes were superior to crossing schemes, mainly due to complete homozygosity, which greatly reduced the total number of disjunct genome segments in the introgression populations. The crossing schemes were more flexible with respect to selection and provided economic alternatives to DH crossing schemes. For the DH crossing schemes, increasing population sizes gradually over backcross generations was advantageous as it reduced the total number of required HT assays compared to constant population sizes. For the crossing schemes, large population sizes in the final backcross generation facilitated selection for the target segments in the final backcross generation and reduced fixation of large donor chromosome segments. The suggested crossing and selection schemes can help to make the genetic diversity of exotic germplasm available for enhancing the genetic variation of narrow-based breeding populations of crops.

[1]  Y. Mano,et al.  Flooding tolerance in interspecific introgression lines containing chromosome segments from teosinte (Zea nicaraguensis) in maize (Zea mays subsp. mays). , 2013, Annals of botany.

[2]  M. Frisch,et al.  A Comparison of Tests for QTL Mapping with Introgression Libraries Containing Overlapping and Nonoverlapping Donor Segments , 2012 .

[3]  T. Lübberstedt,et al.  Application of doubled haploids for target gene fixation in backcross programmes of maize , 2012 .

[4]  R. Nelson,et al.  Analysis of quantitative disease resistance to southern leaf blight and of multiple disease resistance in maize, using near-isogenic lines , 2012, Theoretical and Applied Genetics.

[5]  F. Tardieu,et al.  A Common Genetic Determinism for Sensitivities to Soil Water Deficit and Evaporative Demand: Meta-Analysis of Quantitative Trait Loci and Introgression Lines of Maize1[W][OA] , 2011, Plant Physiology.

[6]  M. Frisch,et al.  Power and false-positive rate in QTL detection with near-isogenic line libraries , 2011, Heredity.

[7]  M. Frisch,et al.  Selection strategies for the development of rye introgression libraries , 2009, Theoretical and Applied Genetics.

[8]  K. Pillen,et al.  Selecting a set of wild barley introgression lines and verification of QTL effects for resistance to powdery mildew and leaf rust , 2008, Theoretical and Applied Genetics.

[9]  R. Meyer,et al.  Construction and analysis of 2 reciprocal Arabidopsis introgression line populations. , 2008, The Journal of heredity.

[10]  V. Korzun,et al.  Establishment of introgression libraries in hybrid rye (Secale cereale L.) from an Iranian primitive accession as a new tool for rye breeding and genomics , 2008, Theoretical and Applied Genetics.

[11]  G. S. Mangat,et al.  Development of high yielding IR64 × Oryza rufipogon (Griff.) introgression lines and identification of introgressed alien chromosome segments using SSR markers , 2008, Euphytica.

[12]  J. Ledeaux,et al.  QTL mapping with near-isogenic lines in maize , 2007, Theoretical and Applied Genetics.

[13]  J. Keurentjes,et al.  Development of a Near-Isogenic Line Population of Arabidopsis thaliana and Comparison of Mapping Power With a Recombinant Inbred Line Population , 2007, Genetics.

[14]  Jean-Marcel Ribaut,et al.  Marker-assisted selection to improve drought adaptation in maize: the backcross approach, perspectives, limitations, and alternatives. , 2006, Journal of experimental botany.

[15]  J. Jia,et al.  Development, utilization of introgression lines using a synthetic wheat as donor , 2006, Theoretical and Applied Genetics.

[16]  Susan McCouch,et al.  Diversifying Selection in Plant Breeding , 2004, PLoS biology.

[17]  W. Powell,et al.  Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. , 2003, Genome.

[18]  D. Zamir.  Improving plant breeding with exotic genetic libraries , 2001, Nature Reviews Genetics.

[19]  S. Lin,et al.  Mapping quantitative trait loci controlling seed dormancy and heading date in rice, Oryza sativa L., using backcross inbred lines , 1998, Theoretical and Applied Genetics.

[20]  P. Stam,et al.  Interference in Genetic Crossing over and Chromosome Mapping. , 1979, Genetics.

[21]  R Core Team,et al.  R: A language and environment for statistical computing. , 2014 .

[22]  Michael O. Pumphrey,et al.  Validating the Fhb1 QTL for fusarium head blight resistance in near-isogenic wheat lines developed from breeding populations , 2007 .

[23]  Yuval Eshed,et al.  A genomic library of Lycopersicon pennellii in L. esculentum: A tool for fine mapping of genes , 2004, Euphytica.

[24]  Z. Chen,et al.  Optimising the construction of a substitution library in Arabidopsis thaliana using computer simulations , 2004, Molecular Breeding.