3D Printing and Biofabrication for Load Bearing Tissue Engineering.

Cell-based direct biofabrication and 3D bioprinting is becoming a dominant technological platform and is suggested as a new paradigm for twenty-first century tissue engineering. These techniques may be our next step in surpassing the hurdles and limitations of conventional scaffold-based tissue engineering, and may offer the industrial potential of tissue engineered products especially for load bearing tissues. Here we present a topically focused review regarding the fundamental concepts, state of the art, and perspectives of this new technology and field of biofabrication and 3D bioprinting, specifically focused on tissue engineering of load bearing tissues such as bone, cartilage, osteochondral and dental tissue engineering.

[1]  T. Boland,et al.  Inkjet printing of viable mammalian cells. , 2005, Biomaterials.

[2]  Michael D Maloney,et al.  Articular Cartilage Biology , 2003, The Journal of the American Academy of Orthopaedic Surgeons.

[3]  Pierre Weiss,et al.  Ectopic bone formation using an injectable biphasic calcium phosphate/Si-HPMC hydrogel composite loaded with undifferentiated bone marrow stromal cells. , 2006, Biomaterials.

[4]  D Basu,et al.  Orthopaedic applications of bone graft & graft substitutes: a review. , 2010, The Indian journal of medical research.

[5]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part II. Rapid prototyping techniques. , 2002, Tissue engineering.

[6]  W. Hennink,et al.  Hydrogels as extracellular matrices for skeletal tissue engineering: state-of-the-art and novel application in organ printing. , 2007, Tissue engineering.

[7]  Eric D. Miller,et al.  Inkjet-based biopatterning of bone morphogenetic protein-2 to spatially control calvarial bone formation. , 2010, Tissue engineering. Part A.

[8]  GeunHyung Kim,et al.  Rapid-prototyped collagen scaffolds reinforced with PCL/β-TCP nanofibres to obtain high cell seeding efficiency and enhanced mechanical properties for bone tissue regeneration , 2012 .

[9]  Ming-Yih Lee,et al.  EFFECTS OF GELATIN MODIFICATION ON RAPID PROTOTYPING PCL SCAFFOLDS FOR CARTILAGE ENGINEERING , 2011 .

[10]  W. Hennink,et al.  Organ printing: the future of bone regeneration? , 2011, Trends in biotechnology.

[11]  Umut A. Gurkan,et al.  Engineering Anisotropic Biomimetic Fibrocartilage Microenvironment by Bioprinting Mesenchymal Stem Cells in Nanoliter Gel Droplets , 2014, Molecular pharmaceutics.

[12]  Lorenza Jaramillo,et al.  Odontogenic cell culture in PEGDA hydrogel scaffolds for use in tooth regeneration protocols. , 2012, Acta odontologica latinoamericana : AOL.

[13]  Sabine Kuchler-Bopp,et al.  Tooth Engineering: Searching for Dental Mesenchymal Cells Sources , 2011, Front. Physio..

[14]  Eric D. Miller,et al.  Microenvironments Engineered by Inkjet Bioprinting Spatially Direct Adult Stem Cells Toward Muscle‐ and Bone‐Like Subpopulations , 2008, Stem cells.

[15]  James J. Yoo,et al.  Hybrid printing of mechanically and biologically improved constructs for cartilage tissue engineering applications , 2012, Biofabrication.

[16]  A. Mikos,et al.  Review: tissue engineering for regeneration of articular cartilage. , 2000, Biomaterials.

[17]  B L Currier,et al.  Biodegradable Polymer Scaffolds for Cartilage Tissue Engineering , 2001, Clinical orthopaedics and related research.

[18]  Anthony Atala,et al.  Isolation of amniotic stem cell lines with potential for therapy , 2007, Nature Biotechnology.

[19]  Samantha J. Polak,et al.  The effect of BMP-2 on micro- and macroscale osteointegration of biphasic calcium phosphate scaffolds with multiscale porosity. , 2010, Acta biomaterialia.

[20]  Guangdong Zhou,et al.  In vitro engineering of human ear-shaped cartilage assisted with CAD/CAM technology. , 2010, Biomaterials.

[21]  T. Boland,et al.  Cell and organ printing 2: fusion of cell aggregates in three-dimensional gels. , 2003, The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology.

[22]  Xiaofeng Cui,et al.  Application of inkjet printing to tissue engineering , 2006, Biotechnology journal.

[23]  C H Lee,et al.  Anatomically Shaped Tooth and Periodontal Regeneration by Cell Homing , 2010, Journal of dental research.

[24]  Scott J. Hollister,et al.  Tailoring the mechanical properties of 3D-designed poly(glycerol sebacate) scaffolds for cartilage applications. , 2010, Journal of biomedical materials research. Part A.

[25]  P. Yelick,et al.  Tissue Engineering of Complex Tooth Structures on Biodegradable Polymer Scaffolds , 2002, Journal of dental research.

[26]  Dong-Woo Cho,et al.  Surface modification with fibrin/hyaluronic acid hydrogel on solid-free form-based scaffolds followed by BMP-2 loading to enhance bone regeneration. , 2011, Bone.

[27]  K W Dalgarno,et al.  Indirect selective laser sintering of an apatite-mullite glass-ceramic for potential use in bone replacement applications , 2004, Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine.

[28]  Jonathan Stringer,et al.  Formation and stability of lines produced by inkjet printing. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[29]  D. Kaplan,et al.  Porosity of 3D biomaterial scaffolds and osteogenesis. , 2005, Biomaterials.

[30]  M Nakamura,et al.  Biomatrices and biomaterials for future developments of bioprinting and biofabrication , 2010, Biofabrication.

[31]  Tarek El-Bialy.  Editorial: a review of tooth tissue engineering studies. , 2012, The open dentistry journal.

[32]  Jos Malda,et al.  Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds. , 2012, Tissue engineering. Part C, Methods.

[33]  Ljubomir Todorovic,et al.  Oral tissue engineering of complex tooth structures on biodegradable DLPLG/beta-TCP scaffolds. , 2004, Advances in experimental medicine and biology.

[34]  Hector F Rios,et al.  Biomimetic hybrid scaffolds for engineering human tooth-ligament interfaces. , 2010, Biomaterials.

[35]  Lilian Costa Anami,et al.  Removable partial dentures: use of rapid prototyping. , 2014, Journal of prosthodontics : official journal of the American College of Prosthodontists.

[36]  Wouter J A Dhert,et al.  Distinct tissue formation by heterogeneous printing of osteo- and endothelial progenitor cells. , 2011, Tissue engineering. Part A.

[37]  Vamsi Krishna Balla,et al.  Microwave‐sintered 3D printed tricalcium phosphate scaffolds for bone tissue engineering , 2013, Journal of tissue engineering and regenerative medicine.

[38]  Arun K Gosain,et al.  Testing the Critical Size in Calvarial Bone Defects: Revisiting the Concept of a Critical-Size Defect , 2010, Plastic and reconstructive surgery.

[39]  Takayuki Ohara,et al.  Evaluation of scaffold materials for tooth tissue engineering. , 2010, Journal of biomedical materials research. Part A.

[40]  Peter F. M. Choong,et al.  Chondrogenesis of Infrapatellar Fat Pad Derived Adipose Stem Cells in 3D Printed Chitosan Scaffold , 2014, PloS one.

[41]  Scott J Hollister,et al.  Scaffold translation: barriers between concept and clinic. , 2011, Tissue engineering. Part B, Reviews.

[42]  F. Melchels,et al.  A review on stereolithography and its applications in biomedical engineering. , 2010, Biomaterials.

[43]  Peter Dubruel,et al.  A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering. , 2012, Biomaterials.

[44]  B. Eppley,et al.  Allograft and Alloplastic Bone Substitutes: A Review of Science and Technology For the Craniomaxillofacial Surgeon , 2005, The Journal of craniofacial surgery.

[45]  Dong-Woo Cho,et al.  Application of microstereolithography in the development of three-dimensional cartilage regeneration scaffolds , 2008, Biomedical microdevices.

[46]  Jan Feijen,et al.  Flexible and elastic scaffolds for cartilage tissue engineering prepared by stereolithography using poly(trimethylene carbonate)-based resins. , 2013, Macromolecular bioscience.

[47]  Colleen L Flanagan,et al.  Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering. , 2005, Biomaterials.

[48]  S. Hollister.  Scaffold Design and Manufacturing: From Concept to Clinic , 2009, Advanced materials.

[49]  Shintaroh Iwanaga,et al.  Three-dimensional inkjet biofabrication based on designed images , 2011, Biofabrication.

[50]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[51]  Hod Lipson,et al.  Additive manufacturing for in situ repair of osteochondral defects , 2010, Biofabrication.

[52]  Paul T. Sharpe,et al.  Stem cells and tooth tissue engineering , 2007, Cell and Tissue Research.

[53]  Maurilio Marcacci,et al.  Scaffold-based repair for cartilage healing: a systematic review and technical note. , 2013, Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association.

[54]  Ralph Holmes,et al.  Review of Bone Substitutes , 2009, Craniomaxillofacial trauma & reconstruction.

[55]  Eric D. Miller,et al.  Engineered spatial patterns of FGF-2 immobilized on fibrin direct cell organization. , 2005, Biomaterials.

[56]  Dong-Woo Cho,et al.  A comparative study on collagen type I and hyaluronic acid dependent cell behavior for osteochondral tissue bioprinting , 2014, Biofabrication.

[57]  K. Nguyen,et al.  A review of materials, fabrication methods, and strategies used to enhance bone regeneration in engineered bone tissues. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.

[58]  Karoly Jakab,et al.  Tissue engineering by self-assembly and bio-printing of living cells , 2010, Biofabrication.

[59]  Jan Feijen,et al.  Designed biodegradable hydrogel structures prepared by stereolithography using poly(ethylene glycol)/poly(D,L-lactide)-based resins. , 2010, Journal of controlled release : official journal of the Controlled Release Society.

[60]  Anthony Atala,et al.  Evaluation of hydrogels for bio-printing applications. , 2013, Journal of biomedical materials research. Part A.

[61]  Xiaoyu Tian,et al.  A brief review of dispensing-based rapid prototyping techniques in tissue scaffold fabrication: role of modeling on scaffold properties prediction , 2009, Biofabrication.

[62]  Arun R. Shrivats,et al.  Bone tissue engineering: state of the union. , 2014, Drug discovery today.

[63]  Scott Hollister,et al.  Tissue-engineered cartilage constructs using composite hyaluronic acid/collagen I hydrogels and designed poly(propylene fumarate) scaffolds. , 2007, Tissue engineering.

[64]  Vladimir Mironov,et al.  Organ printing: promises and challenges. , 2008, Regenerative medicine.

[65]  Makoto Nakamura,et al.  Development of a three-dimensional bioprinter: construction of cell supporting structures using hydrogel and state-of-the-art inkjet technology. , 2009, Journal of biomechanical engineering.

[66]  K. Leong,et al.  The design of scaffolds for use in tissue engineering. Part I. Traditional factors. , 2001, Tissue engineering.

[67]  Christian Krettek,et al.  Enhanced migration of human bone marrow stromal cells in modified collagen hydrogels , 2013, International Orthopaedics.

[68]  R. Banerjee,et al.  Biopolymer-based hydrogels for cartilage tissue engineering. , 2011, Chemical reviews.

[69]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[70]  Yan Xia,et al.  Selective laser sintering fabrication of nano-hydroxyapatite/poly-ε-caprolactone scaffolds for bone tissue engineering applications , 2013, International journal of nanomedicine.

[71]  W Cris Wilson,et al.  Cell and organ printing 1: protein and cell printers. , 2003, The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology.

[72]  Adrian Neagu,et al.  Organ printing: fiction or science. , 2004, Biorheology.

[73]  Vladimir Mironov,et al.  Towards organ printing: engineering an intra-organ branched vascular tree , 2010, Expert opinion on biological therapy.

[74]  Paulo Vinícius Soares,et al.  Rapid prototyping and 3D-virtual models for operative dentistry education in Brazil. , 2013, Journal of dental education.

[75]  Dong-Woo Cho,et al.  An additive manufacturing‐based PCL–alginate–chondrocyte bioprinted scaffold for cartilage tissue engineering , 2015, Journal of tissue engineering and regenerative medicine.

[76]  Hossein Fakhrzadeh,et al.  Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing. , 2012, Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al.

[77]  Minoru Ueda.  [Regeneration of tooth and periodontal tissue using tissue engineering concepts]. , 2003, Nihon rinsho. Japanese journal of clinical medicine.

[78]  Fei Yang,et al.  The effect of composition of calcium phosphate composite scaffolds on the formation of tooth tissue from human dental pulp stem cells. , 2011, Biomaterials.

[79]  Jason A Burdick,et al.  Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering. , 2002, Biomaterials.

[80]  Bin Duan,et al.  Three-dimensional nanocomposite scaffolds fabricated via selective laser sintering for bone tissue engineering. , 2010, Acta biomaterialia.

[81]  Lian Qin,et al.  The Research of Technique on Fabricating Hydrogel Scaffolds for Cartilage Tissue Engineering Based on Stereo-lithography , 2010, 2010 International Conference on Digital Manufacturing & Automation.

[82]  Taka Nakahara,et al.  Potential feasibility of dental stem cells for regenerative therapies: stem cell transplantation and whole-tooth engineering , 2011, Odontology.

[83]  B. Mandelbaum,et al.  Overview of cartilage biology and new trends in cartilage stimulation. , 2013, Foot and ankle clinics.

[84]  D. Cho,et al.  Bioprinting of a mechanically enhanced three-dimensional dual cell-laden construct for osteochondral tissue engineering using a multi-head tissue/organ building system , 2012 .

[85]  Malcolm N. Cooke,et al.  Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth. , 2003, Journal of biomedical materials research. Part B, Applied biomaterials.

[86]  Michael J Yaszemski,et al.  Poly(propylene fumarate) bone tissue engineering scaffold fabrication using stereolithography: effects of resin formulations and laser parameters. , 2007, Biomacromolecules.

[87]  Scott J Hollister,et al.  A comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes. , 2010, Biomaterials.

[88]  Robert C. Breithaupt,et al.  The influence of stereolithographic scaffold architecture and composition on osteogenic signal expression with rat bone marrow stromal cells. , 2011, Biomaterials.

[89]  Frank Beier,et al.  Emerging Frontiers in cartilage and chondrocyte biology. , 2011, Best practice & research. Clinical rheumatology.

[90]  Rocky S Tuan,et al.  Application of visible light-based projection stereolithography for live cell-scaffold fabrication with designed architecture. , 2013, Biomaterials.

[91]  Marc Long,et al.  Bone Graft Substitutes , 2003 .

[92]  P. Yelick,et al.  Accurately shaped tooth bud cell-derived mineralized tissue formation on silk scaffolds. , 2008, Tissue engineering. Part A.

[93]  Avinash S. Bidra,et al.  Computer-aided technology for fabricating complete dentures: systematic review of historical background, current status, and future perspectives. , 2013, The Journal of prosthetic dentistry.

[94]  Daniel C N Chan,et al.  Application of rapid prototyping to operative dentistry curriculum. , 2004, Journal of dental education.

[95]  Thomas Pap,et al.  Cartilage biology, pathology, and repair , 2010, Cellular and Molecular Life Sciences.

[96]  V Mironov,et al.  Biofabrication: a 21st century manufacturing paradigm , 2009, Biofabrication.

[97]  Jeroen Rouwkema,et al.  Vascularization in tissue engineering. , 2008, Trends in biotechnology.

[98]  Antonios G Mikos,et al.  Osteogenic differentiation of rat bone marrow stromal cells cultured on Arg-Gly-Asp modified hydrogels without dexamethasone and beta-glycerol phosphate. , 2005, Biomaterials.

[99]  Amit Bandyopadhyay,et al.  Recent advances in bone tissue engineering scaffolds. , 2012, Trends in biotechnology.

[100]  P. Bártolo,et al.  Additive manufacturing of tissues and organs , 2012 .

[101]  S. Nikzad,et al.  The evolution of rapid prototyping in dentistry: a review , 2009 .

[102]  Cynthia M Smith,et al.  Characterizing environmental factors that impact the viability of tissue-engineered constructs fabricated by a direct-write bioassembly tool. , 2007, Tissue engineering.

[103]  Xiaofeng Jia,et al.  Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds. , 2014, Journal of biomedical materials research. Part A.

[104]  Vladimir Mironov,et al.  Organ printing: tissue spheroids as building blocks. , 2009, Biomaterials.

[105]  Ling Ye,et al.  Mesenchymal Stem Cells and Tooth Engineering , 2009, International Journal of Oral Science.

[106]  Thomas J Webster,et al.  Arginine-glycine-aspartic acid modified rosette nanotube-hydrogel composites for bone tissue engineering. , 2009, Biomaterials.

[107]  Amy J Wagoner Johnson,et al.  A review of the mechanical behavior of CaP and CaP/polymer composites for applications in bone replacement and repair. , 2011, Acta biomaterialia.

[108]  Harri Korhonen,et al.  Preparation of poly(ε-caprolactone)-based tissue engineering scaffolds by stereolithography. , 2011, Acta biomaterialia.

[109]  Donggang Yao,et al.  Chondrogenic derivatives of embryonic stem cells seeded into 3D polycaprolactone scaffolds generated cartilage tissue in vivo. , 2008 .

[110]  Rashid Bashir,et al.  3-D biofabrication using stereolithography for biology and medicine , 2012, 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society.

[111]  Jeremy Mao,et al.  Bone tissue engineering and regeneration: from discovery to the clinic--an overview. , 2011, Tissue engineering. Part B, Reviews.

[112]  A. Bandyopadhyay,et al.  Bone tissue engineering using 3D printing , 2013 .

[113]  S. Hollister.  Porous scaffold design for tissue engineering , 2005, Nature materials.

[114]  H. Luder,et al.  Stem cells for tooth engineering. , 2008, European cells & materials.

[115]  Seeram Ramakrishna,et al.  Nano-featured scaffolds for tissue engineering: a review of spinning methodologies. , 2006, Tissue engineering.

[116]  Hinrich Wiese,et al.  In vitro and in vivo cartilage engineering using a combination of chondrocyte-seeded long-term stable fibrin gels and polycaprolactone-based polyurethane scaffolds. , 2007, Tissue engineering.

[117]  Brian Derby,et al.  Printing and Prototyping of Tissues and Scaffolds , 2012, Science.

[118]  Vladimir Mironov,et al.  Review: bioprinting: a beginning. , 2006, Tissue engineering.

[119]  Ali Khademhosseini,et al.  3D biofabrication strategies for tissue engineering and regenerative medicine. , 2014, Annual review of biomedical engineering.

[120]  Sabine Kuchler-Bopp,et al.  Tissue engineering of tooth crown, root, and periodontium. , 2006, Tissue engineering.

[121]  B. Derby,et al.  Manufacture of biomaterials by a novel printing process , 2002, Journal of materials science. Materials in medicine.

[122]  Sarang Sharma,et al.  Biomaterials in tooth tissue engineering: a review. , 2014, Journal of clinical and diagnostic research : JCDR.

[123]  Brian Mellor,et al.  Multiple material additive manufacturing – Part 1: a review , 2013 .

[124]  Xiaofeng Cui,et al.  Thermal inkjet printing in tissue engineering and regenerative medicine. , 2012, Recent patents on drug delivery & formulation.

[125]  S. Hsu,et al.  Synthesis and 3D Printing of Biodegradable Polyurethane Elastomer by a Water‐Based Process for Cartilage Tissue Engineering Applications , 2014, Advanced healthcare materials.

[126]  Jos Malda,et al.  Extracellular matrix scaffolds for cartilage and bone regeneration. , 2013, Trends in biotechnology.

[127]  Michael J Miller,et al.  The Accuracy of Stereolithography in Planning Craniofacial Bone Replacement , 2003, The Journal of craniofacial surgery.

[128]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[129]  D. Cho,et al.  3D printing of composite tissue with complex shape applied to ear regeneration , 2014, Biofabrication.

[130]  Shuping Peng,et al.  Fabrication of porous polyvinyl alcohol scaffold for bone tissue engineering via selective laser sintering , 2013, Biofabrication.

[131]  Robert L Sah,et al.  Probing the role of multicellular organization in three-dimensional microenvironments , 2006, Nature Methods.

[132]  Eric D. Miller,et al.  Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin. , 2006, Biomaterials.

[133]  Minna Kellomäki,et al.  A review of rapid prototyping techniques for tissue engineering purposes , 2008, Annals of medicine.

[134]  J. L. Gomez Ribelles,et al.  In Vivo Evaluation of 3-Dimensional Polycaprolactone Scaffolds for Cartilage Repair in Rabbits , 2010, The American journal of sports medicine.

[135]  David Dean,et al.  Stereolithographic bone scaffold design parameters: osteogenic differentiation and signal expression. , 2010, Tissue engineering. Part B, Reviews.