Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
暂无分享,去创建一个
[1] T. Eggert,et al. Enantioselective biocatalysis optimized by directed evolution. , 2004, Current opinion in biotechnology.
[2] Martina Pohl,et al. A high-throughput screening assay for hydroxynitrile lyase activity. , 2006, Chemical communications.
[3] M. Reetz,et al. Circular dichroism as a detection method in the screening of enantioselective catalysts. , 2000, Chirality.
[4] Robert Kourist,et al. Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase. , 2008, Angewandte Chemie.
[5] U. Bornscheuer,et al. A high-throughput-screening method for determining the synthetic activity of hydrolases. , 2003, Angewandte Chemie.
[6] J. Aikens,et al. Visualization of enzyme-catalyzed reactions using pH indicators: rapid screening of hydrolase libraries and estimation of the enantioselectivity. , 1999, Bioorganic & medicinal chemistry.
[7] U. Bornscheuer,et al. Directed evolution of a Baeyer–Villiger monooxygenase to enhance enantioselectivity , 2008, Applied Microbiology and Biotechnology.
[8] T. S. Wong,et al. The diversity challenge in directed protein evolution. , 2006, Combinatorial chemistry & high throughput screening.
[9] J. Kingma,et al. Improved enantioselective conversion of styrene epoxides and meso-epoxides through epoxide hydrolases with a mutated nucleophile-flanking residue , 2009 .
[10] Uwe T Bornscheuer,et al. Finding better protein engineering strategies. , 2009, Nature chemical biology.
[11] B. Tidor,et al. Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. , 2007, Chemistry & biology.
[12] D. Weitz,et al. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. , 2009, Lab on a chip.
[13] A. Klibanov,et al. Highly l and d enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method , 2008, Proceedings of the National Academy of Sciences.
[14] Matthias Boese,et al. A practical high-throughput screening system for enantioselectivity by using FTIR spectroscopy. , 2003, Chemistry.
[15] Rohit Sharma,et al. Directed Evolution: An Approach to Engineer Enzymes , 2006, Critical reviews in biotechnology.
[16] John C Whitman,et al. Improving catalytic function by ProSAR-driven enzyme evolution , 2007, Nature Biotechnology.
[17] Manfred T Reetz,et al. A genetic selection system for evolving enantioselectivity of enzymes. , 2008, Chemical communications.
[18] Jon E. Ness,et al. Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently , 2002, Nature Biotechnology.
[19] Manfred T Reetz,et al. Time-Resolved IR-Thermographic Detection and Screening of Enantioselectivity in Catalytic Reactions. , 1998, Angewandte Chemie.
[20] Anna V Hine,et al. Removing the redundancy from randomised gene libraries. , 2003, Journal of molecular biology.
[21] Manfred T. Reetz,et al. A GC-based method for high-throughput screening of enantioselective catalysts , 2001 .
[22] U. Bornscheuer,et al. Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate. , 2003, Chemistry.
[23] Jan Marienhagen,et al. Advances in generating functional diversity for directed protein evolution. , 2009, Current opinion in chemical biology.
[24] Andreas Vogel,et al. Expanding the substrate scope of enzymes: combining mutations obtained by CASTing. , 2006, Chemistry.
[25] Andreas Vogel,et al. Single-cell high-throughput screening to identify enantioselective hydrolytic enzymes. , 2008, Angewandte Chemie.
[26] Alain Wagner,et al. High-throughput screening of enantioselective catalysts by immunoassay. , 2002, Angewandte Chemie.
[27] Manfred T. Reetz,et al. A Practical NMR‐Based High‐Throughput Assay for Screening Enantioselective Catalysts and Biocatalysts , 2002 .
[28] Duncan Graham,et al. Rapid and ultra-sensitive determination of enzyme activities using surface-enhanced resonance Raman scattering , 2004, Nature Biotechnology.
[29] M. Jiménez,et al. Determination of enantiomeric compositions by transient absorption spectroscopy using proteins as chiral selectors. , 2008, Chemistry.
[30] Christopher A. Voigt,et al. Protein building blocks preserved by recombination , 2002, Nature Structural Biology.
[31] Yuguo Zheng,et al. A screening system for active and enantioselective amidase based on its acyl transfer activity , 2007, Applied Microbiology and Biotechnology.
[32] D. Berkowitz,et al. Double-cuvette ISES: in situ estimation of enantioselectivity and relative rate for catalyst screening. , 2005, Journal of the American Chemical Society.
[33] C. Hu,et al. "Cassette" in situ enzymatic screening identifies complementary chiral scaffolds for hydrolytic kinetic resolution across a range of epoxides. , 2007, Angewandte Chemie.
[34] Jean-Louis Reymond,et al. Enzyme assays for high-throughput screening. , 2004, Current opinion in biotechnology.
[35] I. Alfonso,et al. Asymmetric organic synthesis with enzymes , 2008 .
[36] P E Brodelius. Enzyme assays. , 1991, Current opinion in biotechnology.
[37] Lei Zhu,et al. Two methods for the determination of enantiomeric excess and concentration of a chiral sample with a single spectroscopic measurement. , 2007, Chemistry.
[38] K. Hult,et al. High-throughput synthesis and analysis of acylated cyanohydrins. , 2007, Chemistry.
[39] Samrat Mukhopadhyay,et al. Single-molecule biophysics: at the interface of biology, physics and chemistry , 2008, Journal of The Royal Society Interface.
[40] Manfred T Reetz,et al. Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications. , 2004, Proceedings of the National Academy of Sciences of the United States of America.
[41] B. Witholt,et al. High-throughput measurement of the enantiomeric excess of chiral alcohols by using two enzymes. , 2004, Angewandte Chemie.
[42] T. S. Wong,et al. Challenges of the genetic code for exploring sequence space in directed protein evolution , 2007 .
[43] W. Stemmer. DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution. , 1994, Proceedings of the National Academy of Sciences of the United States of America.
[44] Romas J. Kazlauskas,et al. Quick E. A Fast Spectrophotometric Method To Measure the Enantioselectivity of Hydrolases , 1997 .
[45] U. Bornscheuer,et al. High-throughput screening of activity and enantioselectivity of esterases , 2006, Nature Protocols.
[46] Kim D Janda,et al. Chiral sensing using a blue fluorescent antibody. , 2005, Molecular bioSystems.
[47] Manfred T. Reetz,et al. Creation of Enantioselective Biocatalysts for Organic Chemistry by In Vitro Evolution , 1997 .
[48] Xavier Soberón,et al. Combinatorial codon-based amino acid substitutions. , 2004, Nucleic acids research.
[49] M. Truppo,et al. Rapid determination of both the activity and enantioselectivity of ketoreductases. , 2008, Angewandte Chemie.
[50] R. Kazlauskas,et al. Mapping the substrate selectivity and enantioselectivity of esterases from thermophiles , 2004 .
[51] Christophe Ampe,et al. Reducing mutational bias in random protein libraries. , 2005, Analytical biochemistry.
[52] P. Babbitt,et al. Enzyme (re)design: lessons from natural evolution and computation. , 2009, Current opinion in chemical biology.
[53] Wayne M Patrick,et al. Novel methods for directed evolution of enzymes: quality, not quantity. , 2004, Current opinion in biotechnology.
[54] Walter Thiel,et al. Learning from Directed Evolution: Further Lessons from Theoretical Investigations into Cooperative Mutations in Lipase Enantioselectivity , 2007, Chembiochem : a European journal of chemical biology.
[55] Frances H Arnold,et al. Directed enzyme evolution: climbing fitness peaks one amino acid at a time. , 2009, Current opinion in chemical biology.
[56] T. Sonke,et al. A colorimetric method for determination of amino amidase activity. , 2004, Analytical biochemistry.
[57] D. Hilvert,et al. Protein design by directed evolution. , 2008, Annual review of biophysics.
[58] Gjalt W Huisman,et al. Enzyme optimization: moving from blind evolution to statistical exploration of sequence-function space. , 2008, Trends in biotechnology.
[59] U. Bornscheuer,et al. A High-Throughput-Screening Method for the Identification of Active and Enantioselective Hydrolases. , 2001, Angewandte Chemie.
[60] 大植 慎也. Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues , 1999 .
[61] David F Ackerley,et al. Enzyme improvement in the absence of structural knowledge: a novel statistical approach , 2008, The ISME Journal.
[62] M. Reetz,et al. Second-generation MS-based high-throughput screening system for enantioselective catalysts and biocatalysts , 2002 .
[63] U. Bornscheuer,et al. Directed Evolution of an Esterase from Pseudomonas fluorescens Yields a Mutant with Excellent Enantioselectivity and Activity for the Kinetic Resolution of a Chiral Building Block , 2006, Chembiochem : a European journal of chemical biology.
[64] Eric A. Althoff,et al. Kemp elimination catalysts by computational enzyme design , 2008, Nature.
[65] Detlev Belder,et al. Super-High-Throughput Screening of Enantioselective Catalysts by Using Capillary Array Electrophoresis. , 2000, Angewandte Chemie.
[66] Andreas Vogel,et al. Expanding the range of substrate acceptance of enzymes: combinatorial active-site saturation test. , 2005, Angewandte Chemie.
[67] Viktor Stein,et al. New genotype-phenotype linkages for directed evolution of functional proteins. , 2005, Current opinion in structural biology.
[68] J. Bertrán,et al. Computational design of biological catalysts. , 2008, Chemical Society reviews.
[69] R. Kazlauskas,et al. Improving enzyme properties: when are closer mutations better? , 2005, Trends in biotechnology.
[70] Jean-Louis Reymond,et al. The adrenaline test for enzymes. , 2002, Angewandte Chemie.
[71] K. Hult,et al. High-throughput enzymatic method for enantiomeric excess determination of O-acetylated cyanohydrins. , 2006, Journal of the American Chemical Society.
[72] Roberto A Chica,et al. Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design. , 2005, Current opinion in biotechnology.
[73] Eric V Anslyn,et al. A general protocol for creating high-throughput screening assays for reaction yield and enantiomeric excess applied to hydrobenzoin , 2009, Proceedings of the National Academy of Sciences.
[74] B. Hwang,et al. High-throughput screening method for the identification of active and enantioselective ω-transaminases , 2004 .
[75] Manfred T Reetz,et al. Directed evolution of enantioselective enzymes: iterative cycles of CASTing for probing protein-sequence space. , 2006, Angewandte Chemie.
[76] Eric A. Althoff,et al. De Novo Computational Design of Retro-Aldol Enzymes , 2008, Science.
[77] L. Otten,et al. Directed evolution: selecting today's biocatalysts. , 2005, Biomolecular engineering.
[78] U. Bornscheuer,et al. Fluorophoric assay for the high-throughput determination of amidase activity. , 2003, Analytical chemistry.
[79] R. Lerner,et al. High-throughput screening by using a blue-fluorescent antibody sensor. , 2003, Angewandte Chemie.
[80] Jean-Louis Reymond,et al. Recent advances in enzyme assays. , 2004, Trends in biotechnology.
[81] Patrik Johansson,et al. X-ray structure of Candida antarctica lipase A shows a novel lid structure and a likely mode of interfacial activation. , 2008, Journal of molecular biology.
[82] Tjaard Pijning,et al. A Novel Genetic Selection System for Improved Enantioselectivity of Bacillus subtilis Lipase A , 2008, Chembiochem : a European journal of chemical biology.