Electric birefringence and dichroism of acridine orange and methylene blue complexes with polynucleotides

Electro‐optic measurements are reported for the polynucleotides poly A, poly U, poly G, and NaDNA, and their complexes with acridine orange (AO). Measurements were also made on the methylene blue (MB) complex with NaDNA. Poly U, poly G, and NaDNA complexes with AO as well as the NaDNA–MB complex were found to exhibit positive birefringence and perpendicular dichroism indicating the dye molecules are oriented with their long axes perpendicular to the applied electric field. The opposite was found for the AO complex with poly A, which showed positive birefringence and parallel dichroism, indicating that in this case the dye molecules are oriented with their long axes parallel to the field.

[1]  C. T. O'konski,et al.  Theory of the Kerr constant of rigid conducting dipolar macromolecules , 1970 .

[2]  C. T. O'konski,et al.  A SPECTROSCOPIC STUDY OF METHYLENE BLUE MONOMER, DIMER, AND COMPLEXES WITH MONTMORILLONITE , 1963 .

[3]  J. R. Fresco,et al.  Polynucleotides. VI. Molecular properties and conformation of polyribouridylic acid , 1963 .

[4]  I. Tinoco,et al.  The stability of helical polynucleotides: base contributions. , 1962, Journal of molecular biology.

[5]  D. F. Bradley,et al.  Aggregation of Acridine Orange Bound to Polyanions: The Stacking Tendency of Deoxyribonucleic Acids , 1961 .

[6]  Chester T. O'Konski,et al.  ELECTRIC PROPERTIES OF MACROMOLECULES. V. THEORY OF IONIC POLARIZATION IN POLYELECTROLYTES , 1960 .

[7]  M. Lipsett.  EVIDENCE FOR HELICAL STRUCTURE IN POLYURIDYLIC ACID. , 1960, Proceedings of the National Academy of Sciences of the United States of America.

[8]  C. T. O'konski,et al.  Characterization of Helix Pomatia hemocyanin by transient electric birefringence. , 1959, Biochimica et biophysica acta.

[9]  C. T. O'konski,et al.  Electric Properties of Macromolecules. III. Kerr Constants and Rotational Diffusion of Bovine Serum Albumin in Aqueous Solutions1,2 , 1959 .

[10]  C. T. O'konski,et al.  Electric Properties of Macromolecules. IV. Determination of Electric and Optical Parameters from Saturation of Electric Birefringence in Solutions , 1959 .

[11]  D. F. Bradley,et al.  AGGREGATION OF DYES BOUND TO POLYANIONS. , 1959, Proceedings of the National Academy of Sciences of the United States of America.

[12]  R. Warner.  Studies on polynucleotides synthesized by polynucleotide phosphorylase. III. Interaction and ultraviolet absorption. , 1957, The Journal of biological chemistry.

[13]  C. T. O'konski,et al.  Electric Properties of Macromolecules. I. A Study of Electric Polarization in Polyelectrolyte Solutions by Means of Electric Birefringence1 , 1957 .

[14]  C. T. O'konski,et al.  Characterization of the Monomer and Dimer of Tobacco Mosaic Virus by Transient Electric Birefringence1 , 1956 .

[15]  C. T. O'konski,et al.  The Distortion of Aerosol Droplets by an Electric Field , 1953 .