Photooxidase system of Rhodospirillum rubrum III. The role of rhodoquinone and ubiquinone in the activity of preparations of chromatophores and photoreaction centers.

The role of rhodoquinone and ubiquinone in the oxygen photoreducing (photooxidase) activity of Rhodospirillum rubrum was investigated. The sole addition of purified rhodoquinone restored photooxidase activity in isolated chromatophores which had been extracted with organic solvents and which were apparently free of secondary acceptor ubiquinone. Rhodoquinone also enhanced photooxidase activity in photoreaction center preparations from which secondary ubiquinone seemed to have been removed. Those results suggest that rhodoquinone accepts electrons directly from primary ubiquinone during chromatophore photooxidase activity. In contrast, rhodoquinone does not participate in the basal activity of photoreaction center preparations, which seems to result from the autooxidation of both primary and secondary ubiquinone.

[1]  R. Picorel,et al.  The photoreaction center of Rhodospirillum rubrum mutant strain F24.1. , 1980, Biochimica et biophysica acta.

[2]  G. Giménez-Gallego,et al.  A specific role for rhodoquinone in the photosynthetic electron‐transfer system of Rhodospirillum rubrum , 1980 .

[3]  C. Wraight,et al.  THE ROLE OF QUINONES IN BACTERIAL PHOTOSYNTHESIS , 1979 .

[4]  L. Poirier,et al.  Photoreaction center of photosynthetic bacteria. 1. Further chemical characterization of the photoreaction center from Rhodospirillum rubrum. , 1979, Biochemistry.

[5]  W. W. Parson,et al.  The involvement of iron and ubiquinone in electron transfer reactions mediated by reaction centers from photosynthetic bacteria. , 1979, Biochimica et biophysica acta.

[6]  G. Giménez-Gallego,et al.  Photooxidase System of Rhodospirillum rubrum II. Its Role in the Regulation of Cyclic Photophosphorylation , 1978 .

[7]  G. Giménez-Gallego,et al.  Photooxidase system of Rhodospirillum rubrum. I. Photooxidations catalyzed by chromatophores isolated from a mutant deficient in photooxidase activity. , 1977, Biochimica et biophysica acta.

[8]  L. Morrison,et al.  UBIQUINONE AND PHOTOCHEMICAL ACTIVITY IN RHODOSPIRILLUM RUBRUM * , 1977, Photochemistry and photobiology.

[9]  G. Gingras,et al.  The photogeneration of superoxide by isolated photoreaction center from Rhodospirillum rubrum. , 1975, Biochemical and biophysical research communications.

[10]  G. Feher,et al.  Primary acceptor in bacterial photosynthesis: obligatory role of ubiquinone in photoactive reaction centers of Rhodopseudomonas spheroides. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Rockley,et al.  Picosecond detection of an intermediate in the photochemical reaction of bacterial photosynthesis. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[12]  Ramírez Jm,et al.  Origin of the ATP formed during the light-dependent oxygen uptake catalyzed by Rhodospirillum rubrum chromatophores. , 1975 .

[13]  M. E. van der Rest,et al.  The pigment complement of the photosynthetic reaction center isolated from Rhodospirillum rubrum. , 1974, The Journal of biological chemistry.

[14]  William R. Smith,et al.  INTERACTION OF VIOLOGEN DYES WITH CHROMATOPHORES AND REACTION‐CENTER PREPARATIONS FROM RHODOSPIRILLUM RUBRUM , 1974 .

[15]  M. E. van der Rest,et al.  Isolation and partial characterization of P870 reaction center complex from wild type Rhodospirillum rubrum. , 1972, Biochimica et biophysica acta.

[16]  H. Yau,et al.  Photochemical electron transport in photosynthetic reaction centers from Rhodopseudomonas spheroides. I. Kinetics of the oxidation and reduction of P-870 as affected by external factors. , 1972, Biophysical journal.

[17]  R. Clayton.  SPECTROSCOPIC ANALYSIS OF BACTERIOCHLOROPHYLLS IN VITRO AND IN VIVO * , 1966 .

[18]  W. W. Parson,et al.  THE BIOSYNTHESIS OF UBIQUINONE AND RHODOQUINONE FROM P-HYDROXYBENZOATE AND P-HYDROXYBENZALDEHYDE IN RHODOSPIRILLUM RUBRUM. , 1965, The Journal of biological chemistry.

[19]  F. L. Crane,et al.  DETERMINATION OF COENZYME Q (UBIQUINONE). , 1963, Methods of biochemical analysis.

[20]  A. Pumphrey,et al.  A method for determining the concentration of ubiquinone in mitochondrial preparations. , 1960, The Biochemical journal.

[21]  J. Lascelles.  The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. , 1956, The Biochemical journal.

[22]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.