Detection of Zika Virus Infection in Myanmar.

Zika virus (ZIKV), an emerging mosquito-borne flavivirus, causes a dengue-like infection that has recently caught global attention. The infection, which also includes some birth defects, has been documented in the Americas, Pacific Islands, and some parts of Africa and Asia. There are no published reports on local ZIKV transmission in Myanmar. In this study, a total of 462 serum samples from patients and asymptomatic persons were collected in Myanmar from 2004 to 2017. They were analyzed for ZIKV infection by immunoglobulin M capture enzyme-linked immunosorbent assay (ELISA), immunoglobulin G indirect ELISA, neutralization test, real-time polymerase chain reaction (PCR), and conventional PCR. Our study confirmed ZIKV infection in 4.9% of patients with clinical dengue symptoms and in 8.6% of persons who were asymptomatic. This is the first report on ZIKV infection in Myanmar and it suggests the occurrence of ZIKV infection in two geographically distinct sites in this country since at least 2006.

[1]  Thi Bich Hau Vu,et al.  Zika virus infection and microcephaly in Vietnam. , 2017, The Lancet. Infectious diseases.

[2]  K. Morita,et al.  Dengue Associated Acute Encephalitis Syndrome Cases in Son La Province, Vietnam in 2014. , 2017, Japanese journal of infectious diseases.

[3]  G. Ebel,et al.  Impact of simultaneous exposure to arboviruses on infection and transmission by Aedes aegypti mosquitoes , 2017, Nature Communications.

[4]  L. Rodrigues,et al.  High levels of exposure of Zika and Dengue infections detected using plaque reduction neutralization assay in Brazil , 2016 .

[5]  L. Rodrigues,et al.  Association between Zika virus infection and microcephaly in Brazil, January to May, 2016: preliminary report of a case-control study. , 2016, The Lancet. Infectious diseases.

[6]  Marko Kolenc,et al.  Zika Virus Associated with Microcephaly. , 2016, The New England journal of medicine.

[7]  W. Callaghan,et al.  Interim Guidelines for Pregnant Women During a Zika Virus Outbreak--United States, 2016. , 2016, MMWR. Morbidity and mortality weekly report.

[8]  O. O'Connor,et al.  Co-infection with Zika and Dengue Viruses in 2 Patients, New Caledonia, 2014 , 2015, Emerging infectious diseases.

[9]  G. Grard,et al.  Zika Virus in Gabon (Central Africa) – 2007: A New Threat from Aedes albopictus? , 2014, PLoS neglected tropical diseases.

[10]  S. Inoue,et al.  Evaluation of a dengue IgG indirect enzyme-linked immunosorbent assay and a Japanese encephalitis IgG indirect enzyme-linked immunosorbent assay for diagnosis of secondary dengue virus infection. , 2010, Vector borne and zoonotic diseases.

[11]  R. Lanciotti,et al.  Zika virus outbreak on Yap Island, Federated States of Micronesia. , 2009, The New England journal of medicine.

[12]  R. Lanciotti,et al.  Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007 , 2008, Emerging infectious diseases.

[13]  A. Han,et al.  Myanmar Dengue Outbreak Associated with Displacement of Serotypes 2, 3, and 4 by Dengue 1 , 2004, Emerging infectious diseases.

[14]  T. Monath,et al.  Immunoglobulin M antibody capture enzyme-linked immunosorbent assay for diagnosis of St. Louis encephalitis , 1984, Journal of clinical microbiology.