Isolation, Identification and Characterization of Inhibin b Protein of Rattus norvegicus Sertoli Cell Culture as Male Hormonal Contraception Candidate

The purpose of this study is to isolate, identify, and characterize the inhibin b protein of Rattus norvegicusSertoli cell culture. In this research, culture of Sertoli cell using by DMEM medium + FCS 15%. Confirmation ofinhibin b protein from Sertoli cell culture by dot blot technique used inhibin b polyclonal antibody. Identification andcharacterization of inhibin b protein from Sertoli cell culture by SDS PAGE. The result of this studies showed thatSertoli cell culture is the source of inhibin b protein with molecular weight of 175, 163, 153, 81, 78, 66, dan 29 kDa.

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