Doppler optical microangiography improves the quantification of local fluid flow and shear stress within 3-D porous constructs.

Traditional phase-resolved Doppler optical coherence tomography (DOCT) has been reported to have potential for characterizing local fluid flow within a microporous scaffold. In this work, we apply Doppler optical microangiography (DOMAG), a new imaging technique developed by combining optical microangiography (OMAG) with a phase-resolved method, for improved assessment of local fluid flow and its derived parameters, shear stress, and interconnectivity, within highly scattering porous constructs. Compared with DOCT, we demonstrate a dramatic improvement of DOMAG in quantifying flow-related properties within scaffolds in situ for functional tissue engineering.

[1]  Ruikang K. Wang,et al.  Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds. , 2009, Journal of biomedical optics.

[2]  Ruikang K. Wang,et al.  Three dimensional optical angiography. , 2007, Optics express.

[3]  Ruikang K. Wang,et al.  Real-time flow imaging by removing texture pattern artifacts in spectral-domain optical Doppler tomography. , 2006, Optics letters.

[4]  J. Vacanti,et al.  Tissue engineering : Frontiers in biotechnology , 1993 .

[5]  M. Heidaran,et al.  Molecular Signaling in Bioengineered Tissue Microenvironments , 2002, Annals of the New York Academy of Sciences.

[6]  Antonios G. Mikos,et al.  Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[7]  A. Mikos,et al.  Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds. , 1999, Biomaterials.

[8]  B. Sumpio,et al.  Gene regulation by mechanical forces. , 1997, Endothelium : journal of endothelial cell research.

[9]  Robert E Guldberg,et al.  Effects of medium perfusion rate on cell-seeded three-dimensional bone constructs in vitro. , 2003, Tissue engineering.

[10]  Robert L Sah,et al.  Perfusion increases cell content and matrix synthesis in chondrocyte three-dimensional cultures. , 2002, Tissue engineering.

[11]  Ruikang K. Wang,et al.  Doppler optical micro-angiography for volumetric imaging of vascular perfusion in vivo. , 2009, Optics express.

[12]  M. V. van Gemert,et al.  Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography. , 1997, Optics letters.

[13]  M. Sefton,et al.  Tissue engineering. , 1998, Journal of cutaneous medicine and surgery.