Cloning of leukemia associated gene LRP16 in acute myeloid leukemia

Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia (AML).Methods:The Restriction landmark genomic scanning (RLGS),Southern blot, Northern blot and rapid amplification of cDNA end (RACE) technique were performed on genomic DNA or RNA from AML and normal bone marrow (BM) samples.Results:An abnormal 2948bp fragment due to a distinct DNA methylation on NotI site in relapse sample from AML was cloned. The Sequence represents part of a new gene LRP16 which is located on chromosome 11q11. The full length cDNA sequence 980bp was cloned by RACE technique. Conclusion:The results indicate that RLGS and RACE technique are effective methods to find leukemia associated gene in AML. The finding of LRP16 gene is important to investigate mechanism of AML.