论文信息 - Continuous Flow PCR on A Chip

Continuous Flow PCR on A Chip

A micromachined continuous flow reactor has been successfully used to perform the polymerase chain reaction (PCR) at ultra high speed. The device is analogous to an electronic amplifier, and relies on the movement of sample through thermostated temperature zones on a glass microchip. Input and output of material (DNA) is continuous, and amplification independent of input concentration. A 20-cycle PCR amplification of a 176-base pair fragment from the Neisseria Gonorrhoeae DNA gyrase gene (gyrA) was performed at various flow rates, resulting in a total reaction time of between 90 seconds and 18.7 minutes.

[1] L J Kricka,et al. Chip PCR. II. Investigation of different PCR amplification systems in microbabricated silicon-glass chips. , 1996, Nucleic acids research.

[2] M. A. Northrup,et al. Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. , 1996, Analytical chemistry.

[3] H. Berg. Cold Spring Harbor Symposia on Quantitative Biology.: Vol. LII. Evolution of Catalytic Functions. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1987, ISBN 0-87969-054-2, xix + 955 pp., US $150.00. , 1989 .

[4] A Manz,et al. Chemical amplification: continuous-flow PCR on a chip. , 1998, Science.

[5] A. R. Kaiser,et al. Microfabricated structures for integrated DNA analysis. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[6] High - density PCR and beyond , 1996 .

[7] L J Kricka,et al. Analysis of ligase chain reaction products amplified in a silicon-glass chip using capillary electrophoresis. , 1996, Journal of chromatography. A.

[8] D. Garling,et al. Rapid cycle DNA amplification: time and temperature optimization. , 1991, BioTechniques.

[9] K. Mullis,et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. , 1986, Cold Spring Harbor symposia on quantitative biology.