Molecular characterization of a rotaviruslike virus isolated from striped bass (Morone saxatilis)

The characteristics of a rotaviruslike (SBR) virus isolated from striped bass (Morone saxatilis) were examined following purification of viruses from infected cell cultures. Virions had a double-layered capsid of icosahedral symmetry and a diameter of 75 nm. Purified viruses contained five polypeptides ranging in molecular mass from 130 to 35 kDa. None of the structural proteins were glycosylated. Treatment with EDTA did not remove the outer capsid. By using enzymes and a chaotropic agent, it was shown that VP5 was the most external polypeptide. The genome of SBR virus was composed of 11 segments of double-stranded RNA (dsRNA). The electrophoretic pattern of the dsRNA of SBR virus was different from that of reovirus type 1 (Lang) and rotavirus (SA11) dsRNA. The SBR virus was compared with reovirus type 1 and SA11 virus by RNA-RNA blot hybridization. There was no cross-hybridization between any of the genome segments of the SBR, reovirus type 1, or SA11 viruses. Antigenic comparison of SBR virus and SA11 virus by cross-immunoprecipitation and cross-immunofluorescence tests did not show any relationship. These results suggest that SBR virus could represent a new genus within the family Reoviridae.

[1]  S. Samal,et al.  Isolation of a rotavirus from smelt, Osmerus mordax (Mitchell) , 1990 .

[2]  Jen-Leih Wu,et al.  Characteristics of A New Reo-like Virus Isolated from Landlocked Salmon (Oncorhynchus masou Brevoort) , 1989 .

[3]  C. Birch,et al.  Synthesis of human rotavirus polypeptides in cell culture , 1988, Journal of medical virology.

[4]  M. McCrae.  Nucleic acid-based analyses of non-group A rotaviruses. , 2007, Ciba Foundation symposium.

[5]  J. Bridger.  Novel rotaviruses in animals and man. , 2007, Ciba Foundation symposium.

[6]  W. Ahne,et al.  Occurrence of reoviruses in European cyprinid fishes (Tinea tinea Lin.; Leuciscus cephalus Lin.) , 1987 .

[7]  R. Hedrick,et al.  Morphological and biochemical properties of four members of a novel group of reoviruses isolated from aquatic animals. , 1987, The Journal of general virology.

[8]  D. Knudson,et al.  Sequence relatedness of Palyam virus genes to cognates of the Palyam serogroup viruses by RNA-RNA blot hybridization. , 1985, Virology.

[9]  D. Amend,et al.  Characteristics of a Previously Unidentified Virus from Channel Catfish (Ictalurus punctatus) , 1984 .

[10]  A. Bellamy,et al.  Sequence diversity of human rotavirus strains investigated by northern blot hybridization analysis , 1982, Journal of virology.

[11]  D. Graham,et al.  Identification, synthesis, and modifications of simian rotavirus SA11 polypeptides in infected cells , 1982, Journal of virology.

[12]  D. Graham,et al.  Proteolytic enhancement of rotavirus infectivity: molecular mechanisms , 1981, Journal of virology.

[13]  M. McCrae,et al.  Molecular biology of rotaviruses. I. Characterization of basic growth parameters and pattern of macromolecular synthesis , 1981, Journal of virology.

[14]  J. Fryer,et al.  Isolation of a New Reovirus from Chum Salmon in Japan (魚病に関する国際セミナ , 1981 .

[15]  O. Uhlenbeck,et al.  Specific labeling of 3' termini of RNA with T4 RNA ligase. , 1980, Methods in enzymology.

[16]  P. Bowser,et al.  Fish Viruses: A Double-Stranded RNA Icosahedral Virus from a North American Cyprinid , 1979 .

[17]  T. Meyers.  A Reo-like Virus Isolated from Juvenile American Oysters (Crassostrea virginica) , 1979 .

[18]  M. McCrae,et al.  The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. , 1978, Virology.

[19]  R. K. Cross,et al.  Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3 , 1977, Journal of virology.

[20]  S W Kessler,et al.  Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. , 1975, Journal of immunology.

[21]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.