Characterization of Cytolethal Distending Toxin Genes and Expression in Shiga Toxin-Producing Escherichia coli Strains of Non-O157 Serogroups

ABSTRACT We identified cytolethal distending toxin and its gene (cdt) in 17 of 340 non-O157 Shiga toxin-producing Escherichia coli (STEC) strains (serotypes O73:H18, O91:H21, O113:H21, and O153:H18), all of which were eae negative. cdt is either chromosomal and homologous to cdt-V (serotypes O73:H18, O91:H21, and O113:H21) or plasmidborne and identical to cdt-III (serotype O153:H18). Among eae-negative STEC, cdt was associated with disease (P = 0.003).

[1]  C. Pickett,et al.  Interactions of Campylobacter jejuni Cytolethal Distending Toxin Subunits CdtA and CdtC with HeLa Cells , 2003, Infection and Immunity.

[2]  L. Beutin,et al.  Production of Cytolethal Distending Toxins by Pathogenic Escherichia coli Strains Isolated from Human and Animal Sources: Establishment of the Existence of a New cdt Variant (Type IV) , 2003, Journal of Clinical Microbiology.

[3]  A. Friedrich,et al.  Shiga Toxin 1c-Producing Escherichia coli Strains: Phenotypic and Genetic Characterization and Association with Human Disease , 2003, Journal of Clinical Microbiology.

[4]  H. Karch,et al.  Cytolethal Distending Toxin Gene Cluster in Enterohemorrhagic Escherichiacoli O157:H− and O157:H7: Characterization and Evolutionary Considerations , 2003, Infection and Immunity.

[5]  A. Fruth,et al.  Subtyping of pathogenic Escherichia coli strains using flagellar (H)-antigens: serotyping versus fliC polymorphisms. , 2003, International journal of medical microbiology : IJMM.

[6]  C. Clark,et al.  PCR for Detection of cdt-III and the Relative Frequencies of Cytolethal Distending Toxin Variant-Producing Escherichia coli Isolates from Humans and Cattle , 2002, Journal of Clinical Microbiology.

[7]  M. Kuskowski,et al.  Phylogenetic distribution of virulence-associated genes among Escherichia coli isolates associated with neonatal bacterial meningitis in the Netherlands. , 2002, The Journal of infectious diseases.

[8]  A. Friedrich,et al.  Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. , 2002, The Journal of infectious diseases.

[9]  T. Frisan,et al.  The cytolethal distending toxins induce DNA damage and cell cycle arrest. , 2001, Toxicon : official journal of the International Society on Toxinology.

[10]  L. Trabulsi,et al.  Production of cytolethal distending toxin and other virulence characteristics of Escherichia coli strains of serogroup O86. , 2001, Memorias do Instituto Oswaldo Cruz.

[11]  J. Galán,et al.  CdtA, CdtB, and CdtC Form a Tripartite Complex That Is Required for Cytolethal Distending Toxin Activity , 2001, Infection and Immunity.

[12]  J. Galán,et al.  A bacterial toxin that controls cell cycle progression as a deoxyribonuclease I-like protein. , 2000, Science.

[13]  C. Elwell,et al.  DNase I homologous residues in CdtB are critical for cytolethal distending toxin‐mediated cell cycle arrest , 2000, Molecular microbiology.

[14]  M. Albert,et al.  Clonal groups of enteropathogenic Escherichia coli isolated in case-control studies of diarrhoea in Bangladesh. , 2000, Journal of medical microbiology.

[15]  J. Lanser,et al.  Molecular Characterization of a Shiga ToxigenicEscherichia coli O113:H21 Strain Lacking eaeResponsible for a Cluster of Cases of Hemolytic-Uremic Syndrome , 1999, Journal of Clinical Microbiology.

[16]  S. McEwen,et al.  Associations between Virulence Factors of Shiga Toxin-ProducingEscherichia coli and Disease in Humans , 1999, Journal of Clinical Microbiology.

[17]  J. Nougayrède,et al.  A new cytolethal distending toxin (CDT) from Escherichia coli producing CNF2 blocks HeLa cell division in G2/M phase , 1997, Molecular microbiology.

[18]  A. Faruque,et al.  Controlled study of cytolethal distending toxin-producing Escherichia coli infections in Bangladeshi children , 1996, Journal of clinical microbiology.

[19]  E. Pesci,et al.  Escherichia Coli Cytolethal Distending Toxin Cloning, Sequencing, and Expression of The , 2022 .

[20]  J. Kaper,et al.  Cloning and sequencing of the genes encoding Escherichia coli cytolethal distending toxin , 1994, Infection and immunity.

[21]  Y. Takeda,et al.  Cloning and nucleotide sequencing of Vero toxin 2 variant genes from Escherichia coli O91:H21 isolated from a patient with the hemolytic uremic syndrome. , 1990, Microbial pathogenesis.

[22]  H. Lior,et al.  A new heat-labile cytolethal distending toxin (CLDT) produced by Escherichia coli isolates from clinical material. , 1988, Microbial pathogenesis.

[23]  E. Tietze,et al.  Plasmid pattern analysis of natural bacterial isolates and its epidemiological implication , 1983, Journal of Hygiene.

[24]  D J Ayers,et al.  A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. , 1978, Plasmid.