Purpose: [F]FE-PE2I is a novel radioligand for dopamine transporter (DAT) PET imaging. As comparedwith [C]PE2I it shows faster kinetics and more favorable metabolism, with less production of a metabolite with intermediate lipophilicity, which in the case of [C]PE2I has been shown to enter the rat brain. In this studywe aimed at comparing DAT quantificationwith [C]PE2I and [F]FE-PE2I in non-human primates, using kinetic and graphical analyses with the input function of both the parent and the metabolite, to assess the potential contribution of the metabolite.