Overcoming "speed limits" in high throughput chromatographic analysis.

The combination of high speed autosampler technology and ultrafast chromatographic separations enables faster high throughput analysis. With an injection cycle time of 10.6 s, MISER (Multiple Injection in a Single Experimental Run) HPLC-MS analysis of a 96 well microplate can be completed in only 17min. As chromatographic separations in the sub 5s range become increasingly common, even faster autosamplers will be needed to realize further speed improvements in high throughput LC-MS analysis. Indeed with proper hardware sampling approaches, chromatographic analysis of microplates could approach speeds of spectrophotometric plate readers while maintaining the advantage of multicomponent detection and monitoring.

[1]  Christopher J. Welch,et al.  Ultrafast chiral separations for high throughput enantiopurity analysis. , 2017, Chemical communications.

[2]  F. Gasparrini,et al.  Pirkle-type chiral stationary phase on core-shell and fully porous particles: Are superficially porous particles always the better choice toward ultrafast high-performance enantioseparations? , 2016, Journal of chromatography. A.

[3]  C. Welch,et al.  Estimating optimal time for fast chromatographic separations. , 2014, Journal of separation science.

[4]  D. Armstrong,et al.  Advances in high-throughput and high-efficiency chiral liquid chromatographic separations. , 2016, Journal of chromatography. A.

[5]  John M. Woodley,et al.  Engineering of Biocatalysts and Biocatalytic Processes , 2014, Topics in Catalysis.

[6]  T. Berger,et al.  Instrumental Idiosyncrasies Affecting the Performance of Ultrafast Chiral and Achiral Sub/Supercritical Fluid Chromatography. , 2016, Analytical chemistry.

[7]  D. Armstrong,et al.  Ultrafast separation of fluorinated and desfluorinated pharmaceuticals using highly efficient and selective chiral selectors bonded to superficially porous particles. , 2015, Journal of chromatography. A.

[8]  Kevin Bateman,et al.  Nanomole-scale high-throughput chemistry for the synthesis of complex molecules , 2015, Science.

[9]  D. Armstrong,et al.  Gone in seconds: praxis, performance, and peculiarities of ultrafast chiral liquid chromatography with superficially porous particles. , 2015, Analytical chemistry.

[10]  M Farooq Wahab,et al.  Sampling frequency, response times and embedded signal filtration in fast, high efficiency liquid chromatography: A tutorial. , 2016, Analytica chimica acta.

[11]  G. Huisman,et al.  Engineering the third wave of biocatalysis , 2012, Nature.

[12]  Christopher J. Welch,et al.  Comparison of Multiparallel Microfluidic HPLC Instruments for High Throughput Analyses in Support of Pharmaceutical Process Research , 2006 .

[13]  M Farooq Wahab,et al.  Salient Sub-Second Separations. , 2016, Analytical chemistry.

[14]  Gheorghe-Doru Roiban,et al.  Induced axial chirality in biocatalytic asymmetric ketone reduction. , 2013, Journal of the American Chemical Society.

[15]  F. Gasparrini,et al.  Ultra-fast high-efficiency enantioseparations by means of a teicoplanin-based chiral stationary phase made on sub-2 μm totally porous silica particles of narrow size distribution. , 2016, Journal of chromatography. A.

[16]  Mirlinda Biba,et al.  MISER chiral supercritical fluid chromatography for high throughput analysis of enantiopurity. , 2016, Journal of chromatography. A.

[17]  R. King,et al.  Description and validation of a staggered parallel high performance liquid chromatography system for good laboratory practice level quantitative analysis by liquid chromatography/tandem mass spectrometry. , 2002, Rapid communications in mass spectrometry : RCM.

[18]  F. Gasparrini,et al.  Introducing enantioselective ultrahigh-pressure liquid chromatography (eUHPLC): theoretical inspections and ultrafast separations on a new sub-2-μm Whelk-O1 stationary phase. , 2012, Analytical chemistry.

[19]  Christopher J. Welch,et al.  MISER chromatography (multiple injections in a single experimental run): the chromatogram is the graph , 2010 .

[20]  Paul N Devine,et al.  Advances in the enzymatic reduction of ketones. , 2007, Accounts of chemical research.

[21]  C. Welch,et al.  Pushing the speed limit in enantioselective supercritical fluid chromatography. , 2015, Journal of separation science.

[22]  Kendall N Houk,et al.  Rapid catalyst identification for the synthesis of the pyrimidinone core of HIV integrase inhibitors. , 2012, Angewandte Chemie.

[23]  C. Welch,et al.  Evaluation of capsaicin in chili peppers and hot sauces by MISER HPLC-ESIMS , 2014 .

[24]  D. Armstrong,et al.  High efficiency, narrow particle size distribution, sub-2 μm based macrocyclic glycopeptide chiral stationary phases in HPLC and SFC. , 2015, Analytica chimica acta.

[25]  A. C. Costa,et al.  Increasing the instrumental throughput of gas chromatography method using multiple injections in a single experimental run: application in determination of friedelan-3-ol and friedelin in Maytenus ilicifolia. , 2013, Journal of chromatography. A.

[26]  R. Papp,et al.  Evaluating MISER chromatography for a rapid formulation screen. , 2013, Journal of pharmaceutical and biomedical analysis.

[27]  Ying-wu Wang,et al.  Autosampler programming for improved sample throughput in liquid chromatography/mass spectrometry. , 2005, Clinical chemistry.