Lycopene and β-carotene in non-blanched and blanched tomatoes

Tomatoes are an important agricultural commodity worldwide. Tomatoes and tomato-based food products, such as tomato paste, tomato sauce, tomato juice, and tomato soups, are considered as important sources of carotenoids in the human diet. There are many studies showing strong correlations between carotenoid consumption and a reduced risk of cancer and coronary and cardiovascular diseases. The beneficial effects of carotenoids are thought to be due to their role as antioxidants. Lycopene is the predominant carotenoid in tomatoes and exhibits the highest antioxidant activity of known carotenoids. β-carotene is also present in tomatoes, but in a much smaller amount. Lycopene is able to function as an antioxidant and has an activity that is twice as strong as β-carotene, but β-carotene is the major dietary precursor of vitamin A. The waste of tomato processing has been shown to be an excellent and inexpensive source of carotenoids because a large proportion of the carotenoids are discarded along with the peels during the processing of tomatoes into pastes or sauces. Furthermore, some studies indicate that tomato thermal processing (for example, the blanching process) increases carotenoid bioavailability. In the present study, it was established that the best source for purified lycopenes is tomato peels (a by-product of tomato juice production). Data from the present study suggest that the mean lycopene and β-carotene concentrations were higher in the blanched ‘Admiro’ F 1 tomatoes than in the non-blanched tomatoes. The β-carotene concentration in the blanched tomato peels was 24.7 mg/100 g, and there was over 2 times more β-carotene than that in the non-blanched tomato peels. The lycopene concentration in the non-blanched tomato peels was 62.92 mg/100 g, whereas it was 134.04 mg/100 g in the blanched tomato peels. Because a literature review has shown that hexane is the most suitable solvent for extracting carotenoids from the tomato matrix, the stability of lycopene and β-carotene in hexane extracts was evaluated. The samples were stored in the dark at +5°C (±1°C) for 23 days. The all-trans lycopene from the non-blanched tomato peels gradually degraded (by approximately 37% after 23 days), whereas the degradation of the all-trans lycopene from the blanched tomato peels was only approximately 25%. The β-carotene was stable during storage for 23 days. The color of the fresh tomato fruit and after blanching was measured with a portable MiniScan XE Plus spectrophotometer. The intensity of the color was expressed in CIE L*a*b* color coordinates: L* (brightness), a* (redness) and b* (yellowness).

[1]  J. Erdman,et al.  Cis-lycopene is more bioavailable than trans-lycopene in vitro and in vivo in lymph-cannulated ferrets. , 1999, The Journal of nutrition.

[2]  S. Clinton,et al.  The consumption of processed tomato products enhances plasma lycopene concentrations in association with a reduced lipoprotein sensitivity to oxidative damage. , 2003, The Journal of nutrition.

[3]  W. Stahl,et al.  Lycopene is more bioavailable from tomato paste than from fresh tomatoes. , 1997, The American journal of clinical nutrition.

[4]  K. Hsu Evaluation of processing qualities of tomato juice induced by thermal and pressure processing , 2008 .

[5]  G. Yen,et al.  Antioxidant and Pro-Oxidant Effects of Various Tea Extracts , 1997 .

[6]  J. Erdman,et al.  Bioavailability of all-trans and cis-Isomers of Lycopene , 2002 .

[7]  D. Lairon,et al.  Enrichment of tomato paste with 6% tomato peel increases lycopene and beta-carotene bioavailability in men. , 2005, The Journal of nutrition.

[8]  S. Uchiyama,et al.  Effect of carotenoid on calcium content and alkaline phosphatase activity in rat femoral tissues in vitro: the unique anabolic effect of beta-cryptoxanthin. , 2003, Biological & pharmaceutical bulletin.

[9]  J. Giese Color Measurement in Foods , 2003 .

[10]  R. G. Mcguire,et al.  Reporting of Objective Color Measurements , 1992 .

[11]  Lajos Helyes,et al.  Lycopene content and colour of ripening tomatoes as affected by environmental conditions , 2006 .

[12]  S. Agarwal,et al.  Tomato lycopene and its role in human health and chronic diseases. , 2000, CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne.

[13]  Monica Anese,et al.  Effect of peeling and heating on carotenoid content and antioxidant activity of tomato and tomato-virgin olive oil systems , 2003 .

[14]  John Shi,et al.  Lycopene in Tomatoes: Chemical and Physical Properties Affected by Food Processing , 2000, Critical reviews in biotechnology.

[15]  L. Metspalu,et al.  Tomato (Lycopersicon esculentum Mill.) fruit quality and physiological parameters at different ripening stages of Lithuanian cultivars. , 2009 .

[16]  Yeh,et al.  Antioxidant and pro-oxidant effects of lycopene in comparison with beta-carotene on oxidant-induced damage in Hs68 cells. , 2000, The Journal of nutritional biochemistry.

[17]  K. Scott,et al.  Detection and Measurement of Carotenoids by UV/VIS Spectrophotometry , 2001 .

[18]  R. Russell,et al.  Ingestion by Men of a Combined Dose of β-Carotene and Lycopene Does Not Affect the Absorption of β-Carotene but Improves That of Lycopene , 1997 .

[19]  H. Schuchmann,et al.  Thermal processing of carrots: Lycopene stability and isomerisation with regard to antioxidant potential , 2005 .

[20]  B. Chen,et al.  Stability of carotenoids in tomato juice during storage , 2005 .

[21]  J. Hautvast,et al.  Dietary factors that affect the bioavailability of carotenoids. , 2000, The Journal of nutrition.

[22]  Č. Bobinas,et al.  Nutrition quality of different tomato cultivars. , 2009 .

[23]  S. Schwartz,et al.  Lycopene : Chemical and Biological Properties , 1999 .

[24]  Content of carotenoids and physical properties of tomatoes harvested at different ripening stages. , 2008 .

[25]  M. Wahlqvist,et al.  Evaluation of extraction method for the analysis of carotenoids in fruits and vegetables , 1998 .

[26]  B. Shukitt-Hale,et al.  Neurobehavioral aspects of antioxidants in aging , 2000, International Journal of Developmental Neuroscience.

[27]  Y. Kakùda,et al.  Stability of lycopene during food processing and storage. , 2005, Journal of medicinal food.

[28]  Ann Van Loey,et al.  Combined thermal and high pressure colour degradation of tomato puree and strawberry juice , 2007 .

[29]  B. K. Ishida,et al.  A simple, rapid method for HPLC analysis of lycopene isomers. , 2001, Phytochemical analysis : PCA.

[30]  P. Lambelet,et al.  Improving the stability of lycopene Z-isomers in isomerised tomato extracts , 2009 .

[31]  M. Maguer,et al.  Lycopene in tomatoes and tomato pulp fractions , 1996 .