Synthetic antibodies designed on natural sequence landscapes.

[1]  A. Rajpal,et al.  Expanding the ProteOn XPR36 biosensor into a 36-ligand array expedites protein interaction analysis. , 2011, Analytical biochemistry.

[2]  L. Penland,et al.  Determinism and stochasticity during maturation of the zebrafish antibody repertoire , 2011, Proceedings of the National Academy of Sciences.

[3]  J. Reichert,et al.  Development trends for human monoclonal antibody therapeutics , 2010, Nature Reviews Drug Discovery.

[4]  K. Wittrup,et al.  Stability and CDR composition biases enrich binder functionality landscapes. , 2010, Journal of molecular biology.

[5]  A. Ellington,et al.  Rapid construction and characterization of synthetic antibody libraries without DNA amplification , 2010, Biotechnology and bioengineering.

[6]  G. Georgiou,et al.  Selection of full‐length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence‐activated cell sorting screening , 2010, The FEBS journal.

[7]  W. Bialek,et al.  Maximum entropy models for antibody diversity , 2009, Proceedings of the National Academy of Sciences.

[8]  Jan Berka,et al.  Precise determination of the diversity of a combinatorial antibody library gives insight into the human immunoglobulin repertoire , 2009, Proceedings of the National Academy of Sciences.

[9]  J. E. Chung,et al.  Construction of a large synthetic human scFv library with six diversified CDRs and high functional diversity , 2009, Molecules and cells.

[10]  C. Hardman,et al.  Modelling the human immune response: performance of a 1011 human antibody repertoire against a broad panel of therapeutically relevant antigens. , 2008, Protein engineering, design & selection : PEDS.

[11]  Thomas Waldmann,et al.  A novel solid phase technology for high-throughput gene synthesis. , 2008, BioTechniques.

[12]  Andrew C. R. Martin,et al.  Analysis and improvements to Kabat and structurally correct numbering of antibody variable domains. , 2008, Molecular immunology.

[13]  L. Hood.  Wu and Kabat 1970: A Transforming View of Antibody Diversity , 2008, The Journal of Immunology.

[14]  Sachdev S Sidhu,et al.  The intrinsic contributions of tyrosine, serine, glycine and arginine to the affinity and specificity of antibodies. , 2008, Journal of molecular biology.

[15]  R. Scheller,et al.  Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs. , 2008, Journal of immunological methods.

[16]  A. Honegger,et al.  The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies. , 2008, Journal of molecular biology.

[17]  Frederic A. Fellouse,et al.  High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries. , 2007, Journal of molecular biology.

[18]  Susan M. Huse,et al.  Accuracy and quality of massively parallel DNA pyrosequencing , 2007, Genome Biology.

[19]  Sachdev S Sidhu,et al.  Synthetic therapeutic antibodies , 2006, Nature chemical biology.

[20]  K. Rajewsky,et al.  Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production , 2006, The Journal of experimental medicine.

[21]  P. Carter.  Potent antibody therapeutics by design , 2006, Nature Reviews Immunology.

[22]  Sachdev S Sidhu,et al.  Tyrosine plays a dominant functional role in the paratope of a synthetic antibody derived from a four amino acid code. , 2006, Journal of molecular biology.

[23]  P. Katsamba,et al.  Analyzing a kinetic titration series using affinity biosensors. , 2006, Analytical biochemistry.

[24]  A. Merlo,et al.  Design, construction, and characterization of a large synthetic human antibody phage display library , 2005, Proteomics.

[25]  E. H. Cohen,et al.  Generation of high-affinity human antibodies by combining donor-derived and synthetic complementarity-determining-region diversity , 2005, Nature Biotechnology.

[26]  Frederic A. Fellouse,et al.  Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions. , 2004, Journal of molecular biology.

[27]  J. Engler,et al.  Expressed murine and human CDR-H3 intervals of equal length exhibit distinct repertoires that differ in their amino acid composition and predicted range of structures. , 2003, Journal of molecular biology.

[28]  A. Plückthun,et al.  Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides. , 2000, Journal of molecular biology.

[29]  D. Myszka,et al.  Improving biosensor analysis , 1999, Journal of molecular recognition : JMR.

[30]  J. Gerhart,et al.  Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[31]  S. Steinbacher,et al.  Sequence statistics reliably predict stabilizing mutations in a protein domain. , 1994, Journal of molecular biology.

[32]  G. Winter,et al.  In vitro assembly of repertoires of antibody chains on the surface of phage by renaturation. , 1994, Journal of molecular biology.

[33]  C. Barbas,et al.  Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[34]  G. P. Smith,et al.  Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. , 1985, Science.

[35]  T. T. Wu,et al.  AN ANALYSIS OF THE SEQUENCES OF THE VARIABLE REGIONS OF BENCE JONES PROTEINS AND MYELOMA LIGHT CHAINS AND THEIR IMPLICATIONS FOR ANTIBODY COMPLEMENTARITY , 1970, The Journal of experimental medicine.

[36]  J. Marks,et al.  Selection of human antibodies from phage display libraries. , 2004, Methods in molecular biology.

[37]  George Johnson,et al.  Kabat Database and its applications: 30 years after the first variability plot , 2000, Nucleic Acids Res..

[38]  Marie-Paule Lefranc,et al.  IMGT, the international ImMunoGeneTics database , 2001, Nucleic Acids Res..

[39]  M. Geiser,et al.  Selection procedures for nonmatured phage antibodies: a quantitative comparison and optimization strategies. , 1995, Analytical biochemistry.

[40]  A. Plückthun,et al.  Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. , 1994, Nucleic acids research.