Effect of Lufenuron and Oriza sativa Bran Extract on Fraction Protein and Acid Phosphatase Pattern in Haemolymph of Schistocerca gregaria.

The extensive use of insecticides has significant negative aspects such as the development of insect resistance, great threat to environmental health, and increased costs. IGRs and compounds from natural origin considered environmentally safe and less hazardous to non-target biota. So, the current study aimed to evaluate the insecticidal activity of the match (lufenuron) and rice bran (Oriza sative) extract and their effect on fraction protein and acid phosphatase (ACP) pattern of haemolymph of treated Schistocerca gregaria. The present findings cleared that, LC50s estimate for one-day old of 5th nymphs of S. gregaria were 35.66 and 541.71 ppm for lufenuron and O. sativa bran extract, respectively. As to fraction protein pattern, a maximum number of 65 bands were detected, at molecular weight ranged from 6.73 to158.31and 18 unique bands were scored. The epigenetic distance accounted for 1 for lufenuron and 0.76 for Oriza sativa bran extract, 20 days post-treatment. In addition, seven acid phosphatase bands were recognized in treated and untreated 12 samples, with Rf values ranged between 0.02 and 0.86. Three unique bands were scored. In conclusion, the treated nymph with the lethal concentration LC50 of the tested compounds (lufenuron and O. sativa bran extract) showed a significant change in protein fraction and acid phosphatase patterns of haemolymph, which was clearly reflected in high epigenetic differences between control and treated S. gregaria samples.

[1]  R. Bakr,et al.  Effect of the Insect Growth Regulator “ Lufox” on Some Biological Aspects of the Soft Tick Argas persicus (Oken) , 2018 .

[2]  A. Aziz Insecticidal activity of three insect growth regulators towards the dengue and Zika virus vector Aedes aegypti in Saudi Arabia , 2017 .

[3]  M. Amer,et al.  Disturbed acid and alkaline phosphatase activities in desert locust Schistocerca gregaria (Forskal) (Orthoptera: Acrididae) by extracts from the khella plant Ammi visnaga L. (Apiaceae). , 2014 .

[4]  R. Bakr,et al.  The Activity of Some Detoxification Enzymes in Spodoptera Littoralis (Boisd.) Larvae (Lepidoptera – Noctuidae) Treated With Two Different Insect Growth Regulators , 2013 .

[5]  S. Arabia,et al.  Effect of a selected rice bran extract and a chitin synthesis inhibitor on viability of eggs of the house fly Musca domestica Linnaeus. , 2012 .

[6]  R. Bakr,et al.  Toxicological studies for some agricultural waste extracts on mosquito larvae and experimental animals. , 2012, Asian Pacific journal of tropical biomedicine.

[7]  Assar,et al.  Biochemical Effects of Cyromazine on Culex Pipiens Larvae ( Diptera : Culicidae ) , 2012 .

[8]  A. Zibaee,et al.  A juvenile hormone analog, pyriproxifen, affects some biochemical components in the hemolymph and fat bodies of Eurygaster integriceps Puton (Hemiptera: Scutelleridae) , 2011 .

[9]  Julio Caballero,et al.  Insights into the structure of urea-like compounds as inhibitors of the juvenile hormone epoxide hydrolase (JHEH) of the tobacco hornworm Manduca sexta: analysis of the binding modes and structure-activity relationships of the inhibitors by docking and CoMFA calculations. , 2011, Chemosphere.

[10]  W. Akram,et al.  Citrus waste-derived essential oils: alternative larvicides for dengue fever mosquito, Aedes albopictus (Skuse) (Culicidae: Diptera). , 2011 .

[11]  R. Bakr,et al.  Biochemical effects of some agricultural waste extracts against Culex pipiens (Diptera: Culicidae) , 2010 .

[12]  L. Basiouny,et al.  Alterations in the lactate dehydrogenase activity of the desert locust Schistocerca gregaria by the wild plant Fagonia bruguieri (Zygophyllaceae). , 2010 .

[13]  Nehad M. Elbarky,et al.  Effect of Chitin synthesis inhibitors (flufenoxuron) on some biological and biochemical aspects of the cotton leaf worm Spodoptera littoralis Bosid (Lepidoptera: Noctuidae) , 2010 .

[14]  N. Mahdy,et al.  Biochemical changes of the Chitin-synthesis inhibitor, Hexaflumuron (Consult) compound on the desert locust, Schistocerca gregaria (Forskal) , 2009 .

[15]  Abdalla M. Abdalla,et al.  Effect of some plant extracts on the migratory locust Locusta migratoria L. , 2009 .

[16]  .. Z.I.A.AL-Fifi Effect of different neem products on the mortality and fitness of adult Schistocerca gregaria (Forskal). , 2009 .

[17]  R. Bakr,et al.  Efficiency of the chitin synthesis inhibitor lufenuron (cga-184699) on growth, development and morphogenesis of Schistocerca gregaria (orthoptera: acrididae). , 2008 .

[18]  K. Etebari,et al.  Biochemical changes in haemolymph of silkworm larvae due to pyriproxyfen residue , 2007 .

[19]  Li Xiao Diet influences the detoxification enzyme activity of Bactrocera tau (Walker) (Diptera: Tephritidae) , 2007 .

[20]  S. Ghaout,et al.  Biological effects of alkaloïds extracted from three plants of Moroccan arid areas on the desert locust , 2003 .

[21]  P. Murray,et al.  Acid phosphatases , 2002, Molecular pathology : MP.

[22]  M. Lecoq Recent progress in Desert and Migratory Locust management in Africa. Are preventative actions possible ? , 2001 .

[23]  H. Oberlander,et al.  New Perspectives on the Mode of Action of Benzoylphenyl Urea Insecticides , 1998 .

[24]  A. Gregorc,et al.  PROGRAMMED CELL DEATH IN THE HONEY‐BEE (APIS MELLIFERA L.) LARVAE MIDGUT , 1997, Cell biology international.

[25]  P. Jepson,et al.  The effects of the duration of exposure on the toxicity of diflubenzuron, hexaflumuron and teflubenzuron to various stages of II instar Schistocerca gregaria , 1996 .

[26]  F. Fournet,et al.  Effects of the insect growth regulators OMS 2017 and diflubenzuron on the reproductive potential of Aedes aegypti. , 1993, Journal of the American Mosquito Control Association.

[27]  M. Baumgart,et al.  The effects of neem oil and other neem products on nymphs and resulting adults of Schistocerca gregaria, Nomadacris septemfasciata, Locusta migratoria migratorioides, and Zonocercus variegatus , 1993 .

[28]  Osman,et al.  The role of the anti-chitin synthesis, chlorfluazuron (IKI) on the main metabolites during metamorphosis of Schistocerca gregaria Forskal , 1993 .

[29]  H. Wilps,et al.  The effects of neem oil and azadirachtin on mortality, flight activity, and energy metabolism of Schistocerca gregaria forskal—A comparison between laboratory and field locusts , 1992 .

[30]  C. Nicol,et al.  Contact effects of seed oil from the neem tree, Azadirachta indica (A. Juss.), on nymphs of the gregarious phase of the desert locust, Schistocerca gregaria (Forsk.) , 1991 .

[31]  C. Kort,et al.  A juvenile hormone analogue affects the protein pattern of the haemolymph in last-instar larvae of Locusta migratoria , 1991 .

[32]  J. Wendel,et al.  Visualization and Interpretation of Plant Isozymes , 1989 .

[33]  W. S. Abbott,et al.  A method of computing the effectiveness of an insecticide. 1925. , 1925, Journal of the American Mosquito Control Association.