Changes in α-amylase activity in honey during the freeze-drying process

. Honey is a natural product, which is appreciated for its sweetness, high nutritional value and health benefits all over the world. Despite all benefits, the usage of honey in food industry is limited due to its high viscosity. The use of dried honey could be an alternative to liquid honey, and would allow to use it as an additive in a range of many different food products such as sauces, beverages, yogurts etc. There are many parameters, which are used to determinate the quality of honey. a -amylase (diastase) activity is one of the most important criteria to determine the quality and freshness of honey. The aim of the present study was to investigate and compare a -amylase activity in liquid honey samples and freeze-dried honey samples. Overall, 18 honey samples were dehydrated using a freeze-drying method. Freeze-drying of the samples was carried out at – 50.6 °C and the pressure was 0.036 mbar for 72 hours. a -amylase activity in the honey samples was tested using Amylazyme test tablets. The obtained results showed variability in a -amylase activity after the freeze-drying process. As hydroxymethylfurfural (HMF) is another important quality parameter of honey, the content of HMF was determined in the samples by high performance liquid chromatography. In some samples the concentration of HMF after freeze-drying increased and was higher than it is allowed according to the International Honey Commission (for example, 55.75 mg kg -1 ).

[1]  E. Geană,et al.  Establishing authenticity of honey via comprehensive Romanian honey analysis. , 2020, Food chemistry.

[2]  Joscha Kleber,et al.  Determination of trace amounts with ATR FTIR spectroscopy and chemometrics: 5-(hydroxymethyl)furfural in honey. , 2019, Talanta.

[3]  Yeliz Kanar,et al.  HMF formation, diastase activity and proline content changes in bee pollen dried by different drying methods , 2019, LWT.

[4]  L. Ragni,et al.  Investigation of water state during induced crystallization of honey. , 2019, Food chemistry.

[5]  A. Wiktor,et al.  Development and characterization of physical properties of honey-rich powder , 2019, Food and Bioproducts Processing.

[6]  M. Baliga,et al.  Honey in oral health and care: A mini review. , 2019, Journal of oral biosciences.

[7]  I. Norton,et al.  Simultaneous application of ultrasounds and firming agents to improve the quality properties of osmotic + freeze-dried foods , 2018, LWT.

[8]  P. Rocculi,et al.  Kinetic of induced honey crystallization and related evolution of structural and physical properties , 2018, LWT.

[9]  W. Spinosa,et al.  Influence of freezing, pasteurization and maturation on Tiúba honey quality , 2017 .

[10]  Charalampos Proestos,et al.  HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration. , 2017, Food chemistry.

[11]  M. Kilinc,et al.  THE FACILITIES OF SPRAY DRIED HONEY POWDER USE AS A SUBSTITUTE FOR SUGAR IN COOKIE PRODUCTION , 2017 .

[12]  J. Weiss,et al.  Inactivation kinetics of invertase in honey and honey-glucose syrup formulations: effects of temperature and water activity. , 2017, Journal of the science of food and agriculture.

[13]  E. Jakubczyk,et al.  Diastase Activity Retention and Physical Properties of Honey/Arabic Gum Mixtures After Spray Drying and Storage , 2017 .

[14]  Y. Roos,et al.  Dynamic water sorption for the study of amorphous content of vacuum-dried honey powder , 2016 .

[15]  J. Weiss,et al.  Preparation of High‐Grade Powders from Honey–Glucose Syrup Formulations by Vacuum Foam‐Drying Method , 2016 .

[16]  L. V. Gonzaga,et al.  Honey: Chemical composition, stability and authenticity. , 2016, Food chemistry.

[17]  K. Samborska,et al.  Spray Drying of Honey: The Effect of Drying Agents on Powder Properties , 2015 .

[18]  B. Bhandari,et al.  Effect of Addition of Whey Protein Isolate on Spray-Drying Behavior of Honey with Maltodextrin as a Carrier Material , 2013 .

[19]  Jing Zhang,et al.  Effect of honey powder on dough rheology and bread quality. , 2010 .

[20]  N. Rastogi,et al.  Properties of Dried and Intermediate Moisture Honey Products: A Review , 2008 .

[21]  H. Lucero,et al.  Honey diastase activity modified by heating , 2008 .

[22]  Wei Chen,et al.  Preparation of dry honey by microwave–vacuum drying , 2008 .

[23]  N. Rastogi,et al.  Processing of Honey: A Review , 2007 .

[24]  H. Lucero,et al.  Effect of honey high-temperature short-time heating on parameters related to quality, crystallisation phenomena and fungal inhibition , 2004 .

[25]  L.da C Azeredo,et al.  Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins , 2003 .

[26]  B. Adhikari,et al.  STICKINESS IN FOODS: A REVIEW OF MECHANISMS AND TEST METHODS , 2001 .

[27]  S. H. Hasan.  Effect of Storage and Processing Temperatures on Honey Quality , 2013 .

[28]  M. Piazza,et al.  Invertase activity in honey , 1999 .

[29]  A. Sabatini,et al.  Honey quality, methods of analysis and international regulatory standards: Review of the work of the International Honey Commission , 1999 .

[30]  Tony Howes,et al.  Problems associated with spray drying of sugar-rich foods , 1997 .