Coherence effects in digital in-line holographic microscopy.

We analyze the effects of partial coherence in the image formation of a digital in-line holographic microscope (DIHM). The impulse response is described as a function of cross-spectral density of the light used in the space-frequency domain. Numerical simulation based on the applied model shows that a reduction in coherence of light leads to broadening of the impulse response. This is also validated by results from experiments wherein a DIHM is used to image latex beads using light with different spatial and temporal coherence.

[1]  Unnikrishnan Gopinathan,et al.  Generalized in-line digital holographic technique based on intensity measurements at two different planes. , 2008, Applied optics.

[2]  U. Schnars,et al.  Digital recording and numerical reconstruction of holograms: a new method for displaying light in flight. , 1995, Applied optics.

[3]  E. Piano,et al.  Infrared lensless holographic microscope with a vidicon camera for inspection of metallic evaporations on silicon wafers , 2005 .

[4]  Shensheng Han,et al.  On X-ray in-line Gabor holography with a partially coherent source , 1999 .

[5]  M H Jericho,et al.  Tracking particles in four dimensions with in-line holographic microscopy. , 2003, Optics letters.

[6]  Barton,et al.  Removing multiple scattering and twin images from holographic images. , 1991, Physical review letters.

[7]  Wolfgang Osten,et al.  Digital holographic microscopy in the deep (193 nm) ultraviolet. , 2007, Applied optics.

[8]  Bahram Javidi,et al.  Three-dimensional imaging and recognition of microorganism using single-exposure on-line (SEOL) digital holography. , 2005, Optics express.

[9]  Giancarlo Pedrini,et al.  Short-coherence digital microscopy by use of a lensless holographic imaging system. , 2002, Applied optics.

[10]  Wolfgang Osten,et al.  Applications of short-coherence digital holography in microscopy. , 2005, Applied optics.

[11]  E. Wolf.  New theory of partial coherence in the space–frequency domain. Part I: spectra and cross spectra of steady-state sources , 1982 .

[12]  M H Jericho,et al.  Digital in-line holography of microspheres. , 2002, Applied optics.

[13]  Tomasz Kozacki,et al.  Near field hologram registration with partially coherent illumination , 2004 .

[14]  Fink,et al.  Holography with low-energy electrons. , 1990, Physical review letters.

[15]  S. Wilkins,et al.  X-ray phase-contrast microscopy and microtomography. , 2003, Optics express.

[16]  T. Latychevskaia,et al.  Solution to the twin image problem in holography. , 2006, Physical review letters.

[17]  Tomasz Kozacki,et al.  Digital reconstruction of a hologram recorded using partially coherent illumination , 2005 .

[18]  D. Gabor.  A New Microscopic Principle , 1948, Nature.

[19]  L. Repetto,et al.  Lensless digital holographic microscope with light-emitting diode illumination. , 2004, Optics letters.

[20]  Peter Klages,et al.  Digital in-line holographic microscopy. , 2006 .

[21]  E. Wolf,et al.  Coherent-mode representation of Gaussian Schell-model sources and of their radiation fields , 1982 .