The three-dimensional structure of canine parvovirus and its functional implications.

The three-dimensional atomic structure of a single-stranded DNA virus has been determined. Infectious virions of canine parvovirus contain 60 protein subunits that are predominantly VP-2. The central structural motif of VP-2 has the same topology (an eight-stranded antiparallel beta barrel) as has been found in many other icosahedral viruses but represents only about one-third of the capsid protein. There is a 22 angstrom (A) long protrusion on the threefold axes, a 15 A deep canyon circulating about each of the five cylindrical structures at the fivefold axes, and a 15 A deep depression at the twofold axes. By analogy with rhinoviruses, the canyon may be the site of receptor attachment. Residues related to the antigenic properties of the virus are found on the threefold protrusions. Some of the amino termini of VP-2 run to the exterior in full but not empty virions, which is consistent with the observation that some VP-2 polypeptides in full particles can be cleaved by trypsin. Eleven nucleotides are seen in each of 60 symmetry-related pockets on the interior surface of the capsid and together account for 13 percent of the genome.

[1]  Y. Li,et al.  Protein-RNA interactions in an icosahedral virus at 3.0 A resolution. , 1989, Science.

[2]  M G Rossmann,et al.  Comparison of super-secondary structures in proteins. , 1973, Journal of molecular biology.

[3]  P. G. Koch,et al.  The Molecular Biology of Poliovirus , 1985, Springer Vienna.

[4]  M. Rossmann,et al.  Preliminary X-ray crystallographic analysis of canine parvovirus crystals. , 1988, Journal of molecular biology.

[5]  W. Hol,et al.  3.2 Å structure of the copper-containing, oxygen-carrying protein Panulirus interruptus haemocyanin , 1984, Nature.

[6]  Structure of the capsid of Kilham rat virus from small-angle neutron scattering. , 1984, Biochemistry.

[7]  M. Rossmann,et al.  Conservation of the putative receptor attachment site in picornaviruses. , 1988, Virology.

[8]  T A Jones,et al.  Structure of satellite tobacco necrosis virus after crystallographic refinement at 2.5 A resolution. , 1984, Journal of molecular biology.

[9]  J. Casal,et al.  Porcine parvovirus: DNA sequence and genome organization. , 1989, The Journal of general virology.

[10]  M. Rossmann.  The evolution of RNA viruses , 1987, BioEssays : news and reviews in molecular, cellular and developmental biology.

[11]  C. Parrish,et al.  Natural variation of canine parvovirus. , 1985, Science.

[12]  M G Rossmann,et al.  The molecular replacement method. , 1990, Acta crystallographica. Section A, Foundations of crystallography.

[13]  M G Rossmann,et al.  Comparison of protein structures. , 1985, Methods in enzymology.

[14]  T. A. Jones,et al.  Structure of satellite tobacco necrosis virus at 3.0 A resolution. , 1982, Journal of molecular biology.

[15]  M. Murcko,et al.  Evidence for the direct involvement of the rhinovirus canyon in receptor binding. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[16]  D. Filman,et al.  Myristylation of picornavirus capsid protein VP4 and its structural significance , 1987, Nature.

[17]  John E. Johnson,et al.  Structure of southern bean mosaic virus at 2.8 Å resolution , 1980, Nature.

[18]  R. M. Burnett,et al.  Three-dimensional structure of the adenovirus major coat protein hexon. , 1986, Science.

[19]  S. Harrison,et al.  Tomato bushy stunt virus at 2.9 Å resolution , 1978, Nature.

[20]  C. Parrish,et al.  The global spread and replacement of canine parvovirus strains. , 1988, The Journal of general virology.

[21]  D W Hukins,et al.  Structures of synthetic polynucleotides in the A-RNA and A'-RNA conformations: x-ray diffraction analyses of the molecular conformations of polyadenylic acid--polyuridylic acid and polyinosinic acid--polycytidylic acid. , 1973, Journal of molecular biology.

[22]  John E. Johnson,et al.  Structure of a human common cold virus and functional relationship to other picornaviruses , 1985, Nature.

[23]  C. Aquadro,et al.  Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink, and raccoon parvoviruses. , 1988, Virology.

[24]  C. Jongeneel,et al.  A precise map of splice junctions in the mRNAs of minute virus of mice, an autonomous parvovirus , 1986, Journal of virology.

[25]  M. Hayashi,et al.  The parvovirus MVM: a comparison of heavy and light particle infectivity and their density conversion in vitro. , 1976, Virology.

[26]  L. Anderson,et al.  Human parvovirus B19. , 1989, Pediatric annals.

[27]  D. Blow,et al.  The detection of sub‐units within the crystallographic asymmetric unit , 1962 .

[28]  T. A. Jones,et al.  A graphics model building and refinement system for macromolecules , 1978 .

[29]  David Rowlands,et al.  The three-dimensional structure of foot-and-mouth disease virus at 2.9 Å resolution , 1989, Nature.

[30]  G Vriend,et al.  The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating. , 1986, Science.

[31]  M. Rossmann.  The canyon hypothesis. Hiding the host cell receptor attachment site on a viral surface from immune surveillance. , 1989, The Journal of biological chemistry.

[32]  Lars Liljas,et al.  The three-dimensional structure of the bacterial virus MS2 , 1990, Nature.

[33]  John E. Johnson,et al.  Structure of an insect virus at 3.0 Å resolution , 1987, Proteins.

[34]  M. Murthy,et al.  The structure of a T = 1 icosahedral empty particle from southern bean mosaic virus. , 1985, Science.

[35]  F. Eiserling,et al.  Studies on the structure, protein composition and aseembly of the neck of bacteriophage T4. , 1977, Journal of molecular biology.

[36]  P. Tattersall,et al.  A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles , 1989, Journal of virology.

[37]  C. Parrish,et al.  Characterization of a nonhemagglutinating mutant of canine parvovirus. , 1988, Virology.

[38]  E. Jones,et al.  Nucleotide sequence and genome organization of canine parvovirus , 1988, Journal of virology.

[39]  R. Compans,et al.  Polarized entry of canine parvovirus in an epithelial cell line , 1989, Journal of virology.

[40]  A. Klug,et al.  Physical principles in the construction of regular viruses. , 1962, Cold Spring Harbor symposia on quantitative biology.

[41]  Michael G. Rossmann,et al.  Processing oscillation diffraction data for very large unit cells with an automatic convolution technique and profile fitting , 1979 .

[42]  D. W. Jenkins,et al.  Pure red-cell aplasia of 10 years' duration due to persistent parvovirus B19 infection and its cure with immunoglobulin therapy. , 1989, The New England journal of medicine.

[43]  A. Leslie,et al.  Processing and post-refinement of oscillation camera data , 1979 .

[44]  S. Rhode.  Nucleotide sequence of the coat protein gene of canine parvovirus , 1985, Journal of virology.

[45]  D. Ward,et al.  Sequence homology between the structural polypeptides of minute virus of mice. , 1977, Journal of molecular biology.

[46]  John E. Johnson,et al.  Icosahedral RNA virus structure. , 1989, Annual review of biochemistry.

[47]  Jh Parish,et al.  Principles of nucleic acid structure: By W Saenger. pp 556. Springer-Verlag, New York. 1984. DM 79. ISBN 3-540-90761-0 , 1985 .