Malondialdehid (MDA) pada Induksi Cisplatin Tikus Putih Wistar Study of Docking Molecule Flavonoid Coleus Amboinicus and Lowering MDA Concentration on Cisplatin-Induce Wistar Rats

The aims of this study were to evaluate molecular docking of flavonoid Coleus amboinicus (CA) extracts in transforming growth factor­1β and lowering MDA concentration on cisplatin­induced in Wistar rat. Eighteen male Wistar rats (Rattus norvegicus), 3 months of age with a body weight (BW) of 150-200 g, were allocated into three groups, with six animals per group. The control group received aquadest (P0), the treatment group was treated with single doses of cisplatin (5 mg/kg BW., ip) (P1) and received 100 mg/kg BW of the CA extracts (P2) respectively for 7 days. Blood collected for analysis of serum alkaline phospatase (AP), Blood Nitrogen Urea (BUN) and Malondialdehid (MDA) concentrations. The levels of Malondialdehid (MDA) concentrations were analyzed by Avidin­Horseradish Peroxidase (HRP) Sandwich­ELISA. All groups were sacrificed for histopathology. Coleus amboinicus extract significantly decreased the level of AP, BUN and MDA concentrations compared to the control group (p<0.05). The level of MDA could be detected by its level significantly decreased in CA treatment group (p<0.05). Coleus amboinicus (CA) extract has a flavonoid as a marker compound of CA extract has stronger bind to the TGF­β1receptor than its of 3WA_601 ligand in silico analyzed. In histopathological examination showed that cisplatin-induced could alter severe multifocal hemorrhage, interstitial congestion, cell inflammatory, acute glomerular and tubular injury with necrotic cells. Immunohistochemical staining labeled with TGF­1β monoclonal antibodies (Mab) showed marked expression of brownish color aggregates on the surface of tubular epithelial cells and around glomerular mesangial cells in the CA treatment group. This study was concluded that CA extract is inhibited renal tissue injuries by lowering MDA and increasing TGF­1β expression on cisplatin­induced rats. Flavonoid as marker of CA extract has stronger bind to TGF­1β receptor by in silico.

[1]  S. Yıldırım,et al.  Levosimendan ameliorates cisplatin-induced ototoxicity: Rat model. , 2019, International journal of pediatric otorhinolaryngology.

[2]  Changhui Wang,et al.  Effects of Jiangya Xiaoke prescription on TGF-β1 in diabetic nephropathy rats with hypertension and its mechanisms. , 2015, International Journal of Clinical and Experimental Medicine.

[3]  Tao Yang,et al.  EGF Receptor Inhibition Alleviates Hyperuricemic Nephropathy. , 2015, Journal of the American Society of Nephrology : JASN.

[4]  D. Kang,et al.  Uric Acid Puzzle: Dual Role as Anti-oxidantand Pro-oxidant , 2014, Electrolyte & blood pressure : E & BP.

[5]  N. Elsherbiny,et al.  Evaluation of renal protective effects of inhibiting TGF-β type I receptor in a cisplatin-induced nephrotoxicity model. , 2013, European cytokine network.

[6]  P. Negi,et al.  Chemical Composition and Nutraceutical Potential of Indian Borage (Plectranthus amboinicus) Stem Extract , 2013 .

[7]  Hyun Soon Lee.  Pathogenic Role of TGF- β in Diabetic Nephropathy , 2013 .

[8]  O. Kensara.  Protective effect of vitamin C supplementation on oxonate-induced hyperuricemia and renal injury in rats , 2013 .

[9]  Robert Klopfleisch,et al.  Multiparametric and semiquantitative scoring systems for the evaluation of mouse model histopathology - a systematic review , 2013, BMC Veterinary Research.

[10]  J. Staden,et al.  Plectranthus: A plant for the future? , 2011 .

[11]  W. Peng,et al.  Analgesic and Antiinflammatory Activities of the Aqueous Extract from Plectranthus amboinicus (Lour.) Spreng. Both In Vitro and In Vivo , 2011, Evidence-based complementary and alternative medicine : eCAM.

[12]  M. Perazella,et al.  Nephrotoxicity from chemotherapeutic agents: clinical manifestations, pathobiology, and prevention/therapy. , 2010, Seminars in nephrology.

[13]  N. Upwar,et al.  Phyto-Physicochemical Investigation of Leaves of Plectranthus amboinicus (Lour) Spreng , 2010 .

[14]  Dayan,et al.  Cisplatin induced histological changes in renal tissue of rat , 2010 .

[15]  W. May,et al.  A novel role for uric acid in acute kidney injury associated with tumour lysis syndrome. , 2009, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[16]  J. Hughes,et al.  Elevated Uric Acid Increases Blood Pressure in the Rat by a Novel Crystal-Independent Mechanism , 2001, Hypertension.

[17]  A. Bhave,et al.  Effect of cooking on total phenol, total flavonoids and DPPH free radical scavenging potential of Plectranthus amboinicus , 2018 .

[18]  A. Singhai,et al.  RECENT UPDATES ON THE GENUS COLEUS: A REVIEW , 2012 .

[19]  P. Suresh,et al.  Pharmacognostical standardization and toxicity profile of the methanolic leaf extract of Plectranthus amboinicus (Lour) Spreng , 2011 .

[20]  S. Kumaravel,et al.  Determination of Bioactive Components of Plectranthus amboinicus Lour by GC-MS Analysis. M. Uma , 2011 .

[21]  B. Croker,et al.  Effect of elevated serum uric acid on cisplatin-induced acute renal failure. , 2007, American journal of physiology. Renal physiology.