BIOPHYSICAL PROPERTIES OF RAT BRAIN CHOLINE ACETYLTRANSFERASE 1

Abstract— The molecular weight of choline acetyltransferase (ChAc) was determined by using a variety of methods. A molecular weight of 68,000 daltons was found. ChAc is a globular protein with an apparent radius of 3.39 nm (value of the Stokes radius). The existence of a dimeric form and higher aggregates is discussed

[1]  J. Rossier.  PURIFICATION OF RAT BRAIN CHOLINE ACETYLTRANSFERASE 1 , 1976, Journal of neurochemistry.

[2]  F. Wolfgram,et al.  ACTIVATION, INHIBITION AND AGGREGATION OF CHOLINE ACETYLTRANSFERASE (EC 2.3.1.6) , 1974, Journal of neurochemistry.

[3]  H. White,et al.  SEPARATION OF APPARENT MULTIPLE FORMS OF HUMAN BRAIN CHOLINE ACETYLTRANSFERASE BY ISOELECTRIC FOCUSING , 1973, Journal of neurochemistry.

[4]  J. Rossier,et al.  Improved purification of rat brain choline acetyltransferase by using an immunoabsorbent , 1973, FEBS letters.

[5]  F. Wolfgram,et al.  PURIFICATION AND SOME PROPERTIES OF CHOLINE ACETYLTRANSFERASE (EC 2.3.1.6) FROM BOVINE BRAIN , 1973, Journal of neurochemistry.

[6]  V. Glover,et al.  PURIFICATION AND PROPERTIES OF CHOLINE ACETYLTRANSFERASE FROM OX BRAIN STRIATE NUCLEI , 1971, Journal of neurochemistry.

[7]  C. Cavallito,et al.  INHIBITION OF BACTERIAL AND MAMMALIAN CHOLINE ACETYLTRANSFERASES BY STYRYLPYRIDINE ANALOGUES , 1970, Journal of neurochemistry.

[8]  F. Rieger,et al.  L'acétylcholinestérase des organes électriques de Poissons (torpille et gymnote); complexes membranaires , 1969 .

[9]  F. Fonnum,et al.  Radiochemical micro assays for the determination of choline acetyltransferase and acetylcholinesterase activities. , 1969, The Biochemical journal.

[10]  R. McKay,et al.  The behavior of horse liver alcohol dehydrogenase in guanidine hydrochloride solutions. , 1969, The Journal of biological chemistry.

[11]  V. Glover,et al.  Choline acetyltransferase from rat brain. , 1968, The Journal of biological chemistry.

[12]  D. Morris.  THE EFFECT OF SULPHYDRYL AND OTHER DISULPHIDE REDUCING AGENTS ON CHOLINE ACETYLTRANSFERASE ACTIVITY ESTIMATED WITH SYNTHETIC ACETYL‐CoA , 1967, Journal of neurochemistry.

[13]  K. J. Monty,et al.  Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. , 1966, Biochimica et biophysica acta.

[14]  P. Andrews,et al.  Estimation of the molecular weights of proteins by Sephadex gel-filtration. , 1964, The Biochemical journal.

[15]  A. Feinstein,et al.  Sedimentation Behaviour and Molecular Weight of Choline Acetyltransferase , 1964, Nature.

[16]  E. Margoliash,et al.  Interconversion of horse heart cytochrome C monomer and polymers. , 1962, The Journal of biological chemistry.

[17]  B. Ames,et al.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures. , 1961, The Journal of biological chemistry.

[18]  I W SIZER,et al.  A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. , 1952, The Journal of biological chemistry.

[19]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.