Bioethanol Production From Leaves of Quercus Infectoria in Kurdistan Region

Quercus infectoria is one of the most abundant native oak species in the Kurdistan region of Iraq. This study focused on utilizing leaves of Quercus infectoria for ethanol production in the region. A typical three-step conversion process of acid pretreatment, enzymatic hydrolysis, and yeast fermentation was investigated to produce ethanol from the leaves. Under the selected acid pretreatment and enzymatic hydrolysis conditions, the glucose and xylose concentrations in the hydrolysates reached 11.4 g/L and 16.8 g/L, respectively, with the corresponding sugar conversions of 42.8% and 99.8%. A yeast strain, Kluyveromyces marxianus, was used to ferment mono-sugars in the hydrolysates for ethanol production. The ethanol production rate and conversion of K. marxianus in the fermentation were 0.17 g/L/h and 27%. The techno-economic analysis further concluded that a regional ethanol biorefinery can be established in the Zawita sub-district, Iraq to utilize Q. infectoria leaves to produce 200,000,000 kg ethanol/year with a positive energy balance of 745,052,623 MJ/year. The net annual revenue of the biorefinery is $123,692,804. The payback period of the biorefinery is 10 years.

[1]  Héctor A. Ruiz,et al.  Bioethanol production from enzymatic hydrolysates of Agave salmiana leaves comparing S. cerevisiae and K. marxianus , 2019, Renewable Energy.

[2]  F. Bai,et al.  The production of ethanol from lignocellulosic biomass by Kluyveromyces marxianus CICC 1727-5 and Spathaspora passalidarum ATCC MYA-4345 , 2019, Applied Microbiology and Biotechnology.

[3]  Ayhan Demirbas,et al.  Higher heating values of lignin types from wood and non-wood lignocellulosic biomasses , 2017 .

[4]  W. Liao,et al.  Fungal fermentation on anaerobic digestate for lipid-based biofuel production , 2016, Biotechnology for Biofuels.

[5]  Hongzhang Chen,et al.  Industrial technologies for bioethanol production from lignocellulosic biomass , 2016 .

[6]  W. Liao,et al.  A sustainable lignocellulosic biodiesel production integrating solar- and bio-power generation , 2016 .

[7]  K. Kitajima,et al.  Diel patterns of leaf carbohydrate concentrations differ between seedlings and mature trees of two sympatric oak species , 2014 .

[8]  L. Ruohonen,et al.  Effect of oxygenation and temperature on glucose-xylose fermentation in Kluyveromyces marxianus CBS712 strain , 2014, Microbial Cell Factories.

[9]  W. Liao,et al.  Co‐hydrolysis of lignocellulosic biomass for microbial lipid accumulation , 2013, Biotechnology and bioengineering.

[10]  W. Mulbry,et al.  Use of an algal hydrolysate to improve enzymatic hydrolysis of lignocellulose. , 2012, Bioresource technology.

[11]  C. Cardona,et al.  Process Simulation of Fuel Ethanol Production from Lignocellulosics using Aspen Plus , 2011 .

[12]  David R Schmidt,et al.  A Corn Stover Supply Logistics System , 2010 .

[13]  T. Seager,et al.  Comparative Life Cycle Assessment of Lignocellulosic Ethanol Production: Biochemical Versus Thermochemical Conversion , 2010, Environmental management.

[14]  C. Wittmann,et al.  The yeast Kluyveromyces marxianus and its biotechnological potential , 2008, Applied Microbiology and Biotechnology.

[15]  Frank K. Agbogbo,et al.  Fermentation of glucose/xylose mixtures using Pichia stipitis , 2006 .

[16]  Charles E Wyman,et al.  BSA treatment to enhance enzymatic hydrolysis of cellulose in lignin containing substrates , 2006, Biotechnology and bioengineering.

[17]  Ye Sun,et al.  Hydrolysis of lignocellulosic materials for ethanol production: a review. , 2002, Bioresource technology.

[18]  M. Galbe,et al.  Reduced inhibition of enzymatic hydrolysis of steam-pretreated softwood. , 2001, Enzyme and microbial technology.

[19]  W. A. Amos,et al.  Report on Biomass Drying Technology , 1999 .

[20]  Paul E. Minton,et al.  Handbook of Evaporation Technology , 1988 .

[21]  Xue-Fei Cao,et al.  The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials. , 2016, Bioresource technology.

[22]  W. Mosa Forest cover change and migration in Iraqi Kurdistan: A case study from Zawita sub-district , 2016 .

[23]  Jian Zhang,et al.  Cost evaluation of cellulase enzyme for industrial-scale cellulosic ethanol production based on rigorous Aspen Plus modeling , 2015, Bioprocess and Biosystems Engineering.

[24]  Deborah S. Page-Dumroese,et al.  Maintaining soil productivity during forest or biomass-to-energy thinning harvests in the Western United States. , 2010 .

[25]  R. Graham,et al.  Managing organic debris for forest health: Reconciling fire hazard, bark beetles, wildlife, and forest nutrition needs , 2009 .

[26]  Amie D. Sluiter,et al.  Determination of Structural Carbohydrates and Lignin in Biomass , 2004 .

[27]  Awwa,et al.  Standard Methods for the examination of water and wastewater , 1999 .

[28]  Pierre Desprairies,et al.  World Energy Outlook , 1977 .