A membrane-based, high-efficiency, microfluidic debubbler.

In many lab-on-chip applications, it is necessary to remove bubbles from the flow stream. Existing bubble removal strategies have various drawbacks such as low degassing efficiency, long degassing time, large dead volumes, sensitivity to surfactants, and the need for an external vacuum or pressure source. We report on a novel, simple, robust, passive, nozzle-type, membrane-based debubbler that can be readily incorporated into microfluidic devices for rapid degassing. The debubbler is particularly suitable to operate with microfluidic systems made with plastic. The debubbler consists of a hydrophobic, porous membrane that resembles a normally closed valve, which is forced open by the working fluid's pressure. To illustrate the operation of the debubbler, we describe its use in the context of a chip containing a bead array for immunoassays. Our debubbler was able to completely filter gas bubbles out of a segmented flow at rates up to 60 µl s(-1) mm(-2) of membrane area.

[1]  Jie Xu,et al.  Use of a porous membrane for gas bubble removal in microfluidic channels: physical mechanisms and design criteria , 2010, 1005.0107.

[2]  Xingyu Jiang,et al.  A simple PDMS-based microfluidic channel design that removes bubbles for long-term on-chip culture of mammalian cells. , 2010, Lab on a chip.

[3]  John T McDevitt,et al.  A microbead array chemical sensor using capillary-based sample introduction: toward the development of an "electronic tongue". , 2005, Biosensors & bioelectronics.

[4]  Xiaohua Huang,et al.  Electric field directed assembly of high-density microbead arrays. , 2009, Lab on a chip.

[5]  Xianbo Qiu,et al.  A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids. , 2009, Lab on a chip.

[6]  Chih-Ming Ho,et al.  A Methanol-Tolerant Gas-Venting Microchannel for a Microdirect Methanol Fuel Cell , 2007, Journal of Microelectromechanical Systems.

[7]  H. Bau,et al.  Single bead-based electrochemical biosensor. , 2009, Biosensors & bioelectronics.

[8]  Dennis Desheng Meng,et al.  Micropumping of liquid by directional growth and selective venting of gas bubbles. , 2008, Lab on a chip.

[9]  John Spertus,et al.  Use of saliva-based nano-biochip tests for acute myocardial infarction at the point of care: a feasibility study. , 2009, Clinical chemistry.

[10]  Hongrui Jiang,et al.  A debubbler for microfluidics utilizing air-liquid interfaces , 2009 .

[11]  Dafu Cui,et al.  A hard-soft microfluidic-based biosensor flow cell for SPR imaging application. , 2010, Biosensors & bioelectronics.

[12]  Joel Voldman,et al.  An active bubble trap and debubbler for microfluidic systems. , 2008, Lab on a chip.

[13]  J. Voldman,et al.  Microfluidic arrays for logarithmically perfused embryonic stem cell culture. , 2006, Lab on a chip.

[14]  G. Narsimhan,et al.  Characterization of the effect of food emulsifiers on contact angle and dispersibility of lipid coated neutrally buoyant particles , 2008 .

[15]  John T McDevitt,et al.  Disposable polydimethylsiloxane/silicon hybrid chips for protein detection. , 2005, Biosensors & bioelectronics.

[16]  U. Messelhäusser,et al.  Diagnostic Real-Time PCR Assays for the Detection of Emetic Bacillus cereus Strains in Foods and Recent Food-Borne Outbreaks , 2007, Applied and Environmental Microbiology.

[17]  Teruo Fujii,et al.  Cell Culture in 3-Dimensional Microfluidic Structure of PDMS (polydimethylsiloxane) , 2003 .

[18]  John T McDevitt,et al.  Application of microchip assay system for the measurement of C-reactive protein in human saliva. , 2005, Lab on a chip.

[19]  Kemin Wang,et al.  One-dimensional microfluidic beads array for multiple mRNAs expression detection. , 2007, Biosensors & bioelectronics.

[20]  Alexander P Hsiao,et al.  Multiplexed protein detection using antibody-conjugated microbead arrays in a microfabricated electrophoretic device. , 2010, Lab on a chip.

[21]  Jong Hwan Sung,et al.  Prevention of air bubble formation in a microfluidic perfusion cell culture system using a microscale bubble trap , 2009, Biomedical microdevices.

[22]  M. Saito,et al.  An optimal design method for preventing air bubbles in high-temperature microfluidic devices , 2010, Analytical and bioanalytical chemistry.

[23]  Jason A. Thompson,et al.  Polymeric microbead arrays for microfluidic applications , 2010 .

[24]  Haim H Bau,et al.  Microfluidic, bead-based assay: Theory and experiments. , 2010, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.