Enhanced surface plasmon resonance inhibition test (ESPRIT) using latex particles

Abstract The pregnancy hormone human chorionic gonadotropin (hCG) was used as a model antigen to describe a new assay, the Enhanced Surface Plasmon Resonance Inhibition Test (ESPRIT). It was shown that the introduction of sub-micron latex particles instead of anti-antibodies in an enhancement step improved the sensitivity of the assay by a factor of 30. Latex particles are therefore considered to be versatile tools in the development of new immunochemical assays for the detection of any analyte using SPR immunosensors.