OPTIMIZATION OF CATIONIC LIPID MEDIATED GENE TRANSFER: STRUCTURE-FUNCTION, PHYSICO-CHEMICAL, AND CELLULAR STUDIES

ABSTRACT The rationale design aimed at the enhancement of cationic lipid mediated gene transfer is discussed. These improvements are based on the straight evaluation of the structure-activity relationship and on the introduction of new structures. Much attention have been given to the supramolecular structures of the lipid/DNA complexes, to the effect of serum on gene transfer and to the intracellular trafficking of the lipoplexes. Finally new avenue using reducible cationic lipids has been discussed.

[1]  D. Scherman,et al.  Reducible cationic lipids for gene transfer. , 2001, The Biochemical journal.

[2]  Leaf Huang,et al.  Nonviral vectors in the new millennium: delivery barriers in gene transfer. , 2001, Human gene therapy.

[3]  D. Scherman,et al.  Synthesis of new cationic lipids from an unsaturated glycoside scaffold. , 2001, Organic letters.

[4]  D. Scherman,et al.  Critical assessment of the nuclear import of plasmid during cationic lipid‐mediated gene transfer , 2001, The journal of gene medicine.

[5]  D. Scherman,et al.  Synthesis and transfecting properties of a glycosylated polycationic DNA vector. , 2001, Bioorganic & medicinal chemistry letters.

[6]  D. Scherman,et al.  Reduction-sensitive lipopolyamines as a novel nonviral gene delivery system for modulated release of DNA with improved transgene expression. , 2000, Journal of medicinal chemistry.

[7]  D. Scherman,et al.  Synthesis and biological properties of new glycosidic cationic lipids for DNA delivery. , 2000, Bioorganic & medicinal chemistry letters.

[8]  D. Scherman,et al.  Non-electrostatic complexes with DNA: towards novel synthetic gene delivery systems. , 2000, Bioorganic & medicinal chemistry letters.

[9]  D. Scherman,et al.  Plasmid DNA size does not affect the physicochemical properties of lipoplexes but modulates gene transfer efficiency. , 1999, Nucleic acids research.

[10]  J. Hughes,et al.  Use of dithiodiglycolic acid as a tether for cationic lipids decreases the cytotoxicity and increases transgene expression of plasmid DNA in vitro. , 1999, Bioconjugate chemistry.

[11]  D. Scherman,et al.  Coupling of a targeting peptide to plasmid DNA by covalent triple helix formation , 1999, FEBS letters.

[12]  D. Scherman,et al.  Intracellular fate and nuclear targeting of plasmid DNA , 1999, Cell Biology and Toxicology.

[13]  D. Scherman,et al.  Cellular uptake of cationic lipid/DNA complexes by cultured myoblasts and myotubes. , 1999, Biochimica et biophysica acta.

[14]  D. Scherman,et al.  Application of lipids and plasmid design for gene delivery to mammalian cells. , 1998, Current opinion in biotechnology.

[15]  D. Scherman,et al.  Cationic lipid-mediated gene transfer: Analysis of cellular uptake and nuclear import of plasmid DNA , 1998, Cell Biology and Toxicology.

[16]  D. Scherman,et al.  Cationic lipid-mediated gene transfer: effect of serum on cellular uptake and intracellular fate of lipopolyamine/DNA complexes. , 1998, Biochimica et biophysica acta.

[17]  D. Scherman,et al.  Synthesis, activity, and structure--activity relationship studies of novel cationic lipids for DNA transfer. , 1998, Journal of medicinal chemistry.

[18]  J. Hughes,et al.  Introduction of a disulfide bond into a cationic lipid enhances transgene expression of plasmid DNA. , 1998, Biochemical and biophysical research communications.

[19]  D. Scherman,et al.  Virus-sized self-assembling lamellar complexes between plasmid DNA and cationic micelles promote gene transfer. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[20]  D. Scherman,et al.  Formulations which increase the size of lipoplexes prevent serum‐associated inhibition of transfection , 2000, The journal of gene medicine.

[21]  Weiqi Wang,et al.  Cationic Liposomes Containing Disulfide Bonds in Delivery of Plasmid DNA , 1999 .