Near Infrared Raman Spectroscopy (NIRS): A technique for doping control

This paper reports the application of near-infrared Raman spectroscopy to detect ephedrine in biological medium. At present time, the doping control for ephedrine in athletes uses the urinalysis by the gas chromatography/mass spectrometry, with main disadvantage the need of collecting urine and the time delay to obtain results. This work aims to develop a noninvasive technique that will allow to evaluate the concentration of the ephedrine in a real time diagnosis. A Raman system composed by a Ti:Saphire laser pumped by an Argon laser was used, operating at the wavelength of 785 nm, with a laser power of 70 mW at sample position. Raman scattered photons were collected by a f/l.8 spectrometer and a N2-cooled CCD detector. Ephedrine Raman peaks at 1002 and 1603 cm −1 were studied, opening possibility for the identification and quantification of ephedrine. Raman spectra of ephedrine with different concentrations in human urine were taken, and the intensity of the ephedrine peak at 1002 cm −1 was measured as a function of its concentration. It was also studied the Raman spectrum of an urine sample from a Wistar rat, after a subcutaneous inoculation of an ephedrine solution in physiologic serum, at the concentration of 5 mg/ml. It was found that Raman spectroscopy could detect ephedrine in urine at concentrations lower than the doping limit legally permitted by the International Olympic Committee.

[1]  G. Coté,et al.  Noninvasive polarimetric measurement of glucose in cell culture media. , 1997, Journal of biomedical optics.

[2]  Siegfried Schneider,et al.  Combination of high-performance liquid chromatography and SERS detection applied to the analysis of drugs in human blood and urine , 2004 .

[3]  T. Schwenk,et al.  Ephedrine and other stimulants as ergogenic aids. , 2003, Current sports medicine reports.

[4]  C. N. Chiang,et al.  Urine testing for drugs of abuse. , 1986, NIDA research monograph.

[5]  Klaus Danzer,et al.  Application of Near-Infrared Spectroscopy for Non-Invasive Determination of Blood/Tissue Glucose Using Neural Networks , 1995 .

[6]  Bernhard Lendl,et al.  Application of Mid-Infrared Transmission Spectrometry to the Direct Determination of Glucose in Whole Blood , 1998 .

[7]  J. Gaddum,et al.  The Pharmacological Basis of Therapeutics , 1966 .

[8]  T. B. Blank,et al.  Noninvasive prediction of glucose by near-infrared diffuse reflectance spectroscopy. , 1999, Clinical chemistry.

[9]  H. Heise,et al.  Noninvasive Blood Glucose Assay by Near-Infrared Diffuse Reflectance Spectroscopy of the Human Inner Lip , 1993 .

[10]  E. V. Thomas,et al.  Noninvasive glucose monitoring in diabetic patients: a preliminary evaluation. , 1992, Clinical chemistry.

[11]  R. Dupont,et al.  Drug testing by urine and hair analysis: complementary features and scientific issues. , 1995, Forensic science international.

[12]  D. Kell,et al.  Noninvasive, On-Line Monitoring of the Biotransformation by Yeast of Glucose to Ethanol Using Dispersive Raman Spectroscopy and Chemometrics , 1999 .

[13]  R. A. Zângaro,et al.  Analysis of Near-infrared Raman Spectroscopy as a New Technique for a Transcutaneous Non-invasive Diagnosis of Blood Components , 2001, Lasers in Medical Science.

[14]  G. Coté,et al.  The use of polarized laser light through the eye for noninvasive glucose monitoring. , 1999, Diabetes technology & therapeutics.

[15]  Xingguo Chen,et al.  Micellar electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine. , 2004, Journal of separation science.

[16]  G L Coté,et al.  Noninvasive and minimally-invasive optical monitoring technologies. , 2001, The Journal of nutrition.

[17]  Yukihiro Ozaki,et al.  A highly sensitive, compact Raman system without a spectrometer for quantitative analysis of biological samples , 1997 .

[18]  Mark A. Arnold,et al.  Simultaneous Measurements of Glucose, Glutamine, Ammonia, Lactate, and Glutamate in Aqueous Solutions by Near-Infrared Spectroscopy , 1996 .

[19]  Hitoshi Tsuchihashi,et al.  Determination of Methamphetamine and its Related Compounds Using Fourier Transform Raman Spectroscopy , 1997 .

[20]  Martindale.,et al.  THE EXTRA PHARMACOPOEIA , 1937 .

[21]  J. Osterloh.  Testing for Drugs of Abuse , 1993, Clinical pharmacokinetics.

[22]  O. Khalil,et al.  Spectroscopic and clinical aspects of noninvasive glucose measurements. , 1999, Clinical chemistry.

[23]  Francisco Radler de Aquino Neto.  O papel do atleta na sociedade e o controle de dopagem no esporte , 2001 .

[24]  Marta Goldman Feder,et al.  Informações sobre o uso de medicamentos no esporte , 2000 .

[25]  H M Heise.  Non-invasive monitoring of metabolites using near infrared spectroscopy: state of the art. , 1996, Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme.