Fabrication of electrospun cellulose-derived nanofiber membranes with enhanced stability properties of arginase

In this study, cellulose acetate (CA)/polyvinylpyrrolidone (PVP) and CA/PVP/Mn2+ nanofibers were produced by the electrospinning method, and these cellulose-derived membranes were used as carriers for arginase immobilization for the first time. The structural and morphological analysis of these cellulose-derived nanofibers were determined by attenuated total reflection-Fourier to transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and scanning electron microscopy (SEM). After the immobilization process, it was observed that the thermal properties of the cellulose-derived nanofibers especially improved. The optimum temperature value for free arginase was found to be 35 °C, and this value was found to be 40 °C for arginase immobilized cellulose-derived nanofibers. When the free arginase retained only about 35% of its activity at 50 °C and 60 °C after 60 min, arginase immobilized nanofibers protected 65% of their activity under the same conditions. In addition, arginase immobilized CA/PVP and CA/PVP/Mn2+ nanofibers managed to retain 50% of their activity even after 9 and 12 reuses, respectively.

[1]  M. Teke,et al.  β-cyclodextrin based electrospun nanofibers for arginase immobilization and its application in the production of L-ornithine , 2022, Journal of Polymer Research.

[2]  M. Teke,et al.  Zn 2+ ‐Doped PVA Composite Electrospun Nanofiber for Upgrading of Enzymatic Properties of Acetylcholinesterase** , 2020 .

[3]  Mallesh Kurakula,et al.  Moving polyvinyl pyrrolidone electrospun nanofibers and bioprinted scaffolds toward multidisciplinary biomedical applications , 2020 .

[4]  Zhanhu Guo,et al.  Significantly enhanced and precisely modeled thermal conductivity in polyimide nanocomposites with chemically modified graphene via in situ polymerization and electrospinning-hot press technology , 2018 .

[5]  Hafiz M.N. Iqbal,et al.  State-of-the-art protein engineering approaches using biological macromolecules: A review from immobilization to implementation view point. , 2017, International journal of biological macromolecules.

[6]  M. Teke,et al.  A comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stability. , 2017, International journal of biological macromolecules.

[7]  Azman Hassan,et al.  Materials for food packaging applications based on bio-based polymer nanocomposites , 2017 .

[8]  Abdullah M. Asiri,et al.  Recent Development of Chitosan Nanocomposites for Environmental Applications. , 2016, Recent patents on nanotechnology.

[9]  W. Mu,et al.  A coupled system involving arginase and urease for l-ornithine production , 2016 .

[10]  R. Fernández-Lafuente,et al.  Agarose and Its Derivatives as Supports for Enzyme Immobilization , 2016, Molecules.

[11]  J. Kohn,et al.  Nanoparticles and nanofibers for topical drug delivery. , 2016, Journal of controlled release : official journal of the Controlled Release Society.

[12]  S. Bordage,et al.  Investigation of Mammal Arginase Inhibitory Properties of Natural Ubiquitous Polyphenols by Using an Optimized Colorimetric Microplate Assay , 2016, Planta Medica.

[13]  G. Amoabediny,et al.  Effect of Cultivation Time and Medium Condition in Production of Bacterial Cellulose Nanofiber for Urease Immobilization , 2015 .

[14]  Xianhong Yu,et al.  High-level expression of human arginase I in Pichia pastoris and its immobilization on chitosan to produce L-ornithine , 2015, BMC Biotechnology.

[15]  M. Edirisinghe,et al.  Generation of poly(N-vinylpyrrolidone) nanofibres using pressurised gyration. , 2014, Materials science & engineering. C, Materials for biological applications.

[16]  R. Stevanato,et al.  Enzyme immobilization: an update , 2013, Journal of chemical biology.

[17]  Xiao Zhi,et al.  The immunotoxicity of graphene oxides and the effect of PVP-coating. , 2013, Biomaterials.

[18]  Hongbing Deng,et al.  Antibacterial multilayer films fabricated by LBL immobilizing lysozyme and HTCC on nanofibrous mats. , 2013, International journal of biological macromolecules.

[19]  M. A. Hassan,et al.  Fabrication of nanofiber meltblown membranes and their filtration properties , 2013 .

[20]  Ana Cauerhff,et al.  Recent trends in biocatalysis engineering. , 2012, Bioresource technology.

[21]  Daniel N. Tran,et al.  Perspective of Recent Progress in Immobilization of Enzymes , 2011 .

[22]  S. Radiman,et al.  Synthesis and Characterization of Silver/Polyvinilpirrolidone (Ag/PVP) Nanoparticles Using Gamma Irradiation Techniques , 2010 .

[23]  S. Kundu,et al.  Electrospinning: a fascinating fiber fabrication technique. , 2010, Biotechnology advances.

[24]  K. Morishita,et al.  The effect of L-ornithine hydrochloride ingestion on human growth hormone secretion after strength training , 2010 .

[25]  Y. Kajimoto,et al.  L-ornithine supplementation attenuates physical fatigue in healthy volunteers by modulating lipid and amino acid metabolism. , 2008, Nutrition research.

[26]  T. Uchida,et al.  Bitterness suppression of BCAA solutions by L-ornithine. , 2006, Chemical & pharmaceutical bulletin.

[27]  Glenn D Prestwich,et al.  Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds. , 2006, Biomaterials.

[28]  R. Raines,et al.  Reaction mechanism and kinetics of the traceless Staudinger ligation. , 2006, Journal of the American Chemical Society.

[29]  Y. Hsieh,et al.  Enzyme immobilization on ultrafine cellulose fibers via poly(acrylic acid) electrolyte grafts. , 2005, Biotechnology and bioengineering.

[30]  G. Arthanareeswaran,et al.  Synthesis, characterization and thermal studies on cellulose acetate membranes with additive , 2004 .

[31]  Darrell H. Reneker,et al.  Beaded nanofibers formed during electrospinning , 1999 .

[32]  E. Dala,et al.  Immobilization, characterization, and laboratory-scale application of bovine liver arginase , 1994, Applied biochemistry and biotechnology.

[33]  M. Modolell,et al.  Determination of arginase activity in macrophages: a micromethod. , 1994, Journal of immunological methods.

[34]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[35]  C. Conrad,et al.  Thermogravimetric analysis of cellulose , 1968 .

[36]  F. Cimino,et al.  MECHANISM OF THE PROTECTION BY L-ORNITHINE-L-ASPARTATE MIXTURE AND BY L-ARGININE IN AMMONIA INTOXICATION. , 1964, Archives of biochemistry and biophysics.

[37]  Beatriz Brena,et al.  Immobilization of enzymes: a literature survey. , 2013, Methods in molecular biology.

[38]  R. Jalan,et al.  L-Ornithine phenylacetate (OP): a novel treatment for hyperammonemia and hepatic encephalopathy. , 2007, Medical hypotheses.

[39]  L. Cynober,et al.  Use of ornithine α-ketoglutarate in clinical nutrition of elderly patients , 2003 .