Near-Infrared Light Excited and Localized Surface Plasmon Resonance-Enhanced Photoelectrochemical Biosensing Platform for Cell Analysis.

Under near-infrared (NIR) light of 810 nm wavelength for irradiation, a very simple and highly sensitive photoelectrochemical (PEC) biosensing platform has been established using the localized surface plasmon resonance effect of Au nanoparticles (NPs) as signal amplification for the nondestructive analysis of living cells. The water-dispersible Ag2S quantum dots (QDs) synthesized by a one pot method were employed as photoelectrochemically active species, and they exhibited excellent PEC properties irradiated with NIR light which was chosen due to the obvious absorption and fluorescent emission in the NIR light region. After the incorporation of Au NPs on the Ag2S QDs modified ITO electrode, the photoelectric conversion efficiency was greatly increased, at ∼2.5 times that of the pure Ag2S QDs modified electrode. Additionally, 4-mercaptophenylboronic acid (MPBA) molecules, as recognition elements, self-assembled on the electrode surface through Au-S bonds. On the basis of the chemical reaction between sialic acid on the cytomembranes and boric acid of MPBA, the very simple PEC biosensing platform was used for the quantitative determination of MCF-7 cells and dynamic evaluation of cell surface glycan expression under the external stimulus of sialidase. Under NIR light of 810 nm and a potential of 0.15 V, this proposed strategy exhibited a wide linear range from 1 × 102 to 1 × 107 cells/mL, with an experimental detection limit of 100 cells/mL. Importantly, this work provided a promising application for NIR Ag2S QDs coupled with Au NPs in the development of a novel PEC biosensing platform for the nondestructive analysis of biological samples.

[1]  Yunlei Zhou,et al.  Electrochemical immunosensor for N6-methyladenosine detection in human cell lines based on biotin-streptavidin system and silver-SiO2 signal amplification. , 2017, Biosensors & bioelectronics.

[2]  Hong Dai,et al.  A Potentiometric Addressable Photoelectrochemical Biosensor for Sensitive Detection of Two Biomarkers. , 2016, Analytical chemistry.

[3]  H. Ju,et al.  Enhanced Photoelectrochemical Proximity Assay for Highly Selective Protein Detection in Biological Matrixes. , 2016, Analytical chemistry.

[4]  Q. Wei,et al.  Plasmon enhanced photoelectrochemical sensing of mercury (II) ions in human serum based on Au@Ag nanorods modified TiO₂ nanosheets film. , 2016, Biosensors & bioelectronics.

[5]  Yu Qin,et al.  Invoking Direct Exciton-Plasmon Interactions by Catalytic Ag Deposition on Au Nanoparticles: Photoelectrochemical Bioanalysis with High Efficiency. , 2016, Analytical chemistry.

[6]  Jing‐Juan Xu,et al.  Protein Binding Bends the Gold Nanoparticle Capped DNA Sequence: Toward Novel Energy-Transfer-Based Photoelectrochemical Protein Detection. , 2016, Analytical chemistry.

[7]  Feng Li,et al.  A versatile immobilization-free photoelectrochemical biosensor for ultrasensitive detection of cancer biomarker based on enzyme-free cascaded quadratic amplification strategy. , 2016, Biosensors & bioelectronics.

[8]  H. Ju,et al.  Gold Nanoparticles Deposited Polyaniline-TiO2 Nanotube for Surface Plasmon Resonance Enhanced Photoelectrochemical Biosensing. , 2016, ACS applied materials & interfaces.

[9]  Zonghua Wang,et al.  Dye-Sensitized and Localized Surface Plasmon Resonance Enhanced Visible-Light Photoelectrochemical Biosensors for Highly Sensitive Analysis of Protein Kinase Activity. , 2016, Analytical chemistry.

[10]  Jun‐Jie Zhu,et al.  Highly Sensitive and Selective Photoelectrochemical Biosensor for Hg(2+) Detection Based on Dual Signal Amplification by Exciton Energy Transfer Coupled with Sensitization Effect. , 2015, Analytical chemistry.

[11]  Huangxian Ju,et al.  Catalytic Hairpin Assembly-Programmed Porphyrin-DNA Complex as Photoelectrochemical Initiator for DNA Biosensing. , 2015, Analytical chemistry.

[12]  Qingming Shen,et al.  "Signal-on" photoelectrochemical biosensor for sensitive detection of human T-Cell lymphotropic virus type II DNA: dual signal amplification strategy integrating enzymatic amplification with terminal deoxynucleotidyl transferase-mediated extension. , 2015, Analytical chemistry.

[13]  S. Achilefu,et al.  Tunable Ultrasmall Visible-to-Extended Near-Infrared Emitting Silver Sulfide Quantum Dots for Integrin-Targeted Cancer Imaging , 2015, ACS nano.

[14]  A. Manivannan,et al.  Solar hydrogen generation by a CdS-Au-TiO2 sandwich nanorod array enhanced with Au nanoparticle as electron relay and plasmonic photosensitizer. , 2014, Journal of the American Chemical Society.

[15]  Yan Zhang,et al.  Tracking of Transplanted Human Mesenchymal Stem Cells in Living Mice using Near‐Infrared Ag2S Quantum Dots , 2014 .

[16]  N. Marmiroli,et al.  Genome-wide approach in Arabidopsis thaliana to assess the toxicity of cadmium sulfide quantum dots. , 2014, Environmental science & technology.

[17]  Jizheng Wang,et al.  High-performance flexible ultraviolet photoconductors based on solution-processed ultrathin ZnO/Au nanoparticle composite films , 2014, Scientific Reports.

[18]  Yan Zhang,et al.  Application of ZnO/graphene and S6 aptamers for sensitive photoelectrochemical detection of SK-BR-3 breast cancer cells based on a disposable indium tin oxide device. , 2014, Biosensors & bioelectronics.

[19]  S. Kuwabata,et al.  Photofunctional Materials Fabricated with Chalcopyrite-Type Semiconductor Nanoparticles Composed of AgInS2 and Its Solid Solutions. , 2014, The journal of physical chemistry letters.

[20]  P. Reiss,et al.  Ternary and quaternary metal chalcogenide nanocrystals: synthesis, properties and applications , 2013 .

[21]  Kai Yang,et al.  Biodistribution, pharmacokinetics and toxicology of Ag2S near-infrared quantum dots in mice. , 2013, Biomaterials.

[22]  H. Xiong,et al.  One-pot synthesis of water-dispersible Ag2S quantum dots with bright fluorescent emission in the second near-infrared window , 2013, Nanotechnology.

[23]  Peng Wang,et al.  Plasmonic gold nanocrystals coupled with photonic crystal seamlessly on TiO2 nanotube photoelectrodes for efficient visible light photoelectrochemical water splitting. , 2013, Nano letters.

[24]  Haizheng Zhong,et al.  Tuning the Luminescence Properties of Colloidal I-III-VI Semiconductor Nanocrystals for Optoelectronics and Biotechnology Applications. , 2012, The journal of physical chemistry letters.

[25]  Shuo Diao,et al.  In vivo fluorescence imaging with Ag2S quantum dots in the second near-infrared region. , 2012, Angewandte Chemie.

[26]  Jiangtian Li,et al.  Photocatalytic activity enhanced by plasmonic resonant energy transfer from metal to semiconductor. , 2012, Journal of the American Chemical Society.

[27]  Dai-Wen Pang,et al.  Water-soluble Ag(2)S quantum dots for near-infrared fluorescence imaging in vivo. , 2012, Biomaterials.

[28]  H. Dai,et al.  Ag2S quantum dot: a bright and biocompatible fluorescent nanoprobe in the second near-infrared window. , 2012, ACS nano.

[29]  H. Ju,et al.  Highly sensitive fluorescent analysis of dynamic glycan expression on living cells using glyconanoparticles and functionalized quantum dots. , 2011, Analytical chemistry.

[30]  Baoxin Li,et al.  A simple and sensitive method for visual detection of heparin using positively-charged gold nanoparticles as colorimetric probes. , 2011, Chemical communications.

[31]  Yan Zhang,et al.  Near-infrared photoluminescent Ag2S quantum dots from a single source precursor. , 2010, Journal of the American Chemical Society.

[32]  Wei Cheng,et al.  A simple electrochemical cytosensor array for dynamic analysis of carcinoma cell surface glycans. , 2009, Angewandte Chemie.

[33]  Akira Matsumoto,et al.  Noninvasive sialic acid detection at cell membrane by using phenylboronic acid modified self-assembled monolayer gold electrode. , 2009, Journal of the American Chemical Society.

[34]  C. Bertozzi,et al.  Chemical Approaches To Perturb, Profile, and Perceive Glycans , 2009, Accounts of chemical research.

[35]  V. Haldankar,et al.  RBC membrane composition in insulin dependent diabetes mellitus in context of oxidative stress , 2008, Indian Journal of Clinical Biochemistry.

[36]  J. Hirabayashi,et al.  Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins , 2003, Nature Biotechnology.

[37]  E. Coronado,et al.  The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment , 2003 .

[38]  R. Weissleder.  A clearer vision for in vivo imaging , 2001, Nature Biotechnology.

[39]  C. Bertozzi,et al.  Chemical Glycobiology , 2001, Science.

[40]  Yan Zhang,et al.  In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window. , 2014, Biomaterials.